WB: HeLa, Hep G2, Raji and HEK293 cell lysates. IHC: Human breast cancer tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human CIRP Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.3 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant CIRP Antibody (Ab096565) - Western Blot All lanes: Recombinant CIRP Antibody (Ab096565) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 15.1 s
Associated Targets
CIRBPTbio Cold-inducible RNA-binding protein 0 Activities
1.Sheikh, M S MS and 6 more authors.. (1997) Identification of several human homologs of hamster DNA damage-inducible transcripts. Cloning and characterization of a novel UV-inducible cDNA that codes for a putative RNA-binding protein.. The Journal of biological chemistry, (17):[PMID:9334257]
2.Nishiyama, H H and 6 more authors.. (1997) Cloning and characterization of human CIRP (cold-inducible RNA-binding protein) cDNA and chromosomal assignment of the gene.. Gene, (19):[PMID:9434172]
3.Yang, C C and Carrier, F F.. (2001) The UV-inducible RNA-binding protein A18 (A18 hnRNP) plays a protective role in the genotoxic stress response.. The Journal of biological chemistry, (14):[PMID:11574538]
4.Grimwood, Jane J and 97 more authors.. (2004) The DNA sequence and biology of human chromosome 19.. Nature, (1):[PMID:15057824]
5.Wellmann, Sven S and 7 more authors.. (2004) Oxygen-regulated expression of the RNA-binding proteins RBM3 and CIRP by a HIF-1-independent mechanism.. Journal of cell science, (1):[PMID:15075239]
6.Yang, Ruiqing R, Weber, David J DJ and Carrier, France F.. (2006) Post-transcriptional regulation of thioredoxin by the stress inducible heterogenous ribonucleoprotein A18.. Nucleic acids research, [PMID:16513844]
7.Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5):[PMID:18669648]
8.Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18):[PMID:19690332]
9.Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4):[PMID:23186163]
10.Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16):[PMID:24275569]
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