A synthetic peptide derived from human Claudin 1 (AA 180-211)
Positive Control
WB: A431 and Hep G2 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Claudin 1 Antibody, Recombinant, could be used for WB and so on. Application WB: 1/500-1/2000 Protein Function Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391). (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594).
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
1.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Claudin 1 Antibody (Ab096695) - Western Blot All lanes: Recombinant Claudin 1 Antibody (Ab096695) at 1/2000 dilution Samples: Lysates at 10 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 23 kDa Observed band size: 20 kDa Exposure time: 6.9 s
1.Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C et al.. (2003) The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.. Genome Res, 13 (10):(2265-70). [PMID:12975309]
2.Swisshelm, K K and 5 more authors.. (1999) SEMP1, a senescence-associated cDNA isolated from human mammary epithelial cells, is a member of an epithelial membrane protein superfamily.. Gene, (21):[PMID:9931503]
3.Halford, S S and 5 more authors.. (2000) Assignment of claudin-1 (CLDN1) to human chromosome 3q28-->q29 with somatic cell hybrids.. Cytogenetics and cell genetics, [PMID:10828592]
4.Krämer, F F, White, K K, Kubbies, M M, Swisshelm, K K and Weber, B H BH.. (2000) Genomic organization of claudin-1 and its assessment in hereditary and sporadic breast cancer.. Human genetics, [PMID:11071387]
5.Hadj-Rabia, Smail S and 10 more authors.. (2004) Claudin-1 gene mutations in neonatal sclerosing cholangitis associated with ichthyosis: a tight junction disease.. Gastroenterology, [PMID:15521008]
6.Feldmeyer, L L and 5 more authors.. (2006) Confirmation of the origin of NISCH syndrome.. Human mutation, [PMID:16619213]
7.Evans, Matthew J MJ and 9 more authors.. (2007) Claudin-1 is a hepatitis C virus co-receptor required for a late step in entry.. Nature, (12):[PMID:17325668]
8.Harris, Helen J HJ and 11 more authors.. (2010) Claudin association with CD81 defines hepatitis C virus entry.. The Journal of biological chemistry, (2):[PMID:20375010]
9.Lupberger, Joachim J and 25 more authors.. (2011) EGFR and EphA2 are host factors for hepatitis C virus entry and possible targets for antiviral therapy.. Nature medicine, [PMID:21516087]
10.Kirschner, Nina N and 5 more authors.. (2013) Contribution of tight junction proteins to ion, macromolecule, and water barrier in keratinocytes.. The Journal of investigative dermatology, [PMID:23407391]
11.Bonander, Nicklas and 13 more authors.. (2013) Production, purification and characterization of recombinant, full-length human claudin-1.. PloS one, [PMID:23704991]
12.Che, Pulin P, Tang, Hengli H and Li, Qianjun Q.. (2013) The interaction between claudin-1 and dengue viral prM/M protein for its entry.. Virology, [PMID:24074594]
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