Recombinant; Rabbit anti Human CLIP170 Antibody; WB, IHC, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab096865-10μl | 10μl | In stock | $66.90 | |
| Ab096865-50μl | 50μl | In stock | $189.90 | |
| Ab096865-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $289.90 | |
| Ab096865-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human CLIP170 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Recombinant CLIP170 Antibody - Primary antibody, specific to CLIP1, Rabbit IgG |
|---|---|
| Synonyms | CAP GLY domain containing linker protein 1 antibody; CAP-Gly domain-containing linker protein 1 antibody; CLIP 170 antibody; CLIP antibody; CLIP-170 antibody; CLIP1 antibody; CLIP1_HUMAN antibody; CYLN1 antibody; cytoplasmic linker 1 antibody; Cytoplasmic |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | CLIP1 |
| Immunogen | A synthetic peptide derived from human CLIP170 (AA 1394-1408). |
| Positive Control | WB: HeLa, A549, MCF7 and HUVEC cell lysates. IHC: Human tonsil tissue. ICC/IF: HeLa ceIIs. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human CLIP170 Antibody, Recombinant, could be used for WB, IHC, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant CLIP170 Antibody (Ab096865) - Western Blot
All lanes: Recombinant CLIP170 Antibody (Ab096865) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 162 kDa
Observed band size: 140, 167,195 kDa
Exposure time: 45.8 s
Recombinant CLIP170 Antibody (Ab096865) - IF/ICC
Immunocytochemistry analysis of CLIP17 (green) in HeLa using Recombinant CLIP170 Antibody (Ab096865), and cells were counterstained with DAPI (blue).
Recombinant CLIP170 Antibody (Ab096865) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant CLIP170 Antibody (Ab096865). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0102404 | Certificate of Analysis | Jan 10, 2024 | Ab096865 |
| ZJ24F0102403 | Certificate of Analysis | Jan 10, 2024 | Ab096865 |
| 1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 2. Pierre, P P, Scheel, J J, Rickard, J E JE and Kreis, T E TE.. (1992) CLIP-170 links endocytic vesicles to microtubules.. Cell, (18): [PMID:1356075] |
| 3. Bilbe, G G and 9 more authors.. (1992) Restin: a novel intermediate filament-associated protein highly expressed in the Reed-Sternberg cells of Hodgkin's disease.. The EMBO journal, [PMID:1600942] |
| 4. Choi, Jae H JH and 5 more authors.. (2002) The FKBP12-rapamycin-associated protein (FRAP) is a CLIP-170 kinase.. EMBO reports, [PMID:12231510] |
| 5. Sahin, Ugur U and 5 more authors.. (2002) Hodgkin and Reed-Sternberg cell-associated autoantigen CLIP-170/restin is a marker for dendritic cells and is involved in the trafficking of macropinosomes to the cytoskeleton, supporting a function-based concept of Hodgkin and Reed-Sternberg cells.. Blood, (1): [PMID:12433698] |
| 6. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974] |
| 7. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243] |
| 8. Mishima, Masaki M and 6 more authors.. (2007) Structural basis for tubulin recognition by cytoplasmic linker protein 170 and its autoinhibition.. Proceedings of the National Academy of Sciences of the United States of America, (19): [PMID:17563362] |
| 9. Hayashi, Ikuko I, Plevin, Michael J MJ and Ikura, Mitsuhiko M.. (2007) CLIP170 autoinhibition mimics intermolecular interactions with p150Glued or EB1.. Nature structural & molecular biology, [PMID:17828275] |
| 10. Weisbrich, Anke A and 7 more authors.. (2007) Structure-function relationship of CAP-Gly domains.. Nature structural & molecular biology, [PMID:17828277] |
| 11. Slep, Kevin C KC and Vale, Ronald D RD.. (2007) Structural basis of microtubule plus end tracking by XMAP215, CLIP-170, and EB1.. Molecular cell, (21): [PMID:17889670] |
| 12. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 13. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 14. Li, Hongchang H and 5 more authors.. (2010) Polo-like kinase 1 phosphorylation of p150Glued facilitates nuclear envelope breakdown during prophase.. Proceedings of the National Academy of Sciences of the United States of America, (17): [PMID:20679239] |
| 15. van der Vaart, Babet B and 10 more authors.. (2011) SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase.. The Journal of cell biology, (13): [PMID:21646404] |
| 16. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 17. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
