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Size

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Ab098161-10μl

10μl

In stock

$66.90

Ab098161-50μl

50μl

In stock

$199.90

Ab098161-100μl

100μl

Available within 8-12 weeks(?)

$299.90

Ab098161-1ml

1ml

Available within 8-12 weeks(?)

$2,499.90

Basic Description

Product Name	Recombinant CUG-BP1 Antibody - Primary antibody, specific to CUG-BP1, Rabbit IgG
Synonyms	50 kDa Nuclear polyadenylated RNA binding protein antibody\|50 kDa nuclear polyadenylated RNA-binding protein antibody\|Bruno like 2 antibody\|bruno like protein 2 antibody\|Bruno-like protein 2 antibody\|BRUNOL 2 antibody\|BRUNOL2 antibody\|CELF 1 antibody\|CELF
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species	Rabbit
Specificity	CUG-BP1
Immunogen	A synthetic peptide derived from human CUG-BP1 (AA 1-14)
Positive Control	WB: HeLa, Ramos Hep G2, SK-N-SH, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissues. ICC/IF: HT1080 cells.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human CUG-BP1 Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver (By similarity). Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	0.2 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant CUG-BP1 Antibody (Ab098161) - Western Blot
All lanes: Recombinant CUG-BP1 Antibody (Ab098161) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 52, 55 kDa
Exposure time: 31.2 s

Recombinant CUG-BP1 Antibody (Ab098161) - IF/ICC
Immunocytochemistry analysis of CUG BP1 (green) in HT-1080 using Recombinant CUG-BP1 Antibody (Ab098161), and DAPI (blue).

Recombinant CUG-BP1 Antibody (Ab098161) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant CUG-BP1 Antibody (Ab098161). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets

CELF1 Tbio CUGBP Elav-like family member 1 0 Activities

Discover Target: CELF1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

NSRP1 Tbio Nuclear speckle splicing regulatory protein 1 0 Activities

Discover Target: NSRP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000
ICC/IF	1/50-1/200
IHC	1/50 - 1/100

Certificates

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0303213	Certificate of Analysis	Mar 14, 2024	Ab098161
ZJ24F0303212	Certificate of Analysis	Mar 14, 2024	Ab098161

FAQs and Articles

FAQ

Immunohistochemical staining protocol for frozen samples (IHC-Fr)

Categories: Protocols

Tags:

IHC Monoclonal antibodies Immunohistochemistry Frozen section Recombinant Antibodies

Immunohistochemistry (IHC) staining antigen retrieval protocol

Categories: Protocols

Tags:

IHC Monoclonal antibodies Immunohistochemistry Recombinant Antibodies Antigen Retrieval

References

1. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811]
2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
3. Timchenko, L T LT and 8 more authors.. (1996) Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy.. Nucleic acids research, (15): [PMID:8948631]
4. Timchenko, N A NA, Welm, A L AL, Lu, X X and Timchenko, L T LT.. (1999) CUG repeat binding protein (CUGBP1) interacts with the 5' region of C/EBPbeta mRNA and regulates translation of C/EBPbeta isoforms.. Nucleic acids research, (15): [PMID:10536163]
5. Good, P J PJ, Chen, Q Q, Warner, S J SJ and Herring, D C DC.. (2000) A family of human RNA-binding proteins related to the Drosophila Bruno translational regulator.. The Journal of biological chemistry, (15): [PMID:10893231]
6. Timchenko, N A NA and 5 more authors.. (2001) RNA CUG repeats sequester CUGBP1 and alter protein levels and activity of CUGBP1.. The Journal of biological chemistry, (16): [PMID:11124939]
7. Ladd, A N AN, Charlet, N N and Cooper, T A TA.. (2001) The CELF family of RNA binding proteins is implicated in cell-specific and developmentally regulated alternative splicing.. Molecular and cellular biology, [PMID:11158314]
8. Takahashi, N N and 8 more authors.. (2001) Coexpression of the CUG-binding protein reduces DM protein kinase expression in COS cells.. Journal of biochemistry, [PMID:11686919]
9. Paillard, Luc L, Legagneux, Vincent V and Beverley Osborne, H H.. () A functional deadenylation assay identifies human CUG-BP as a deadenylation factor.. Biology of the cell, [PMID:12799066]
10. Iakova, Polina P and 6 more authors.. (2004) Competition of CUGBP1 and calreticulin for the regulation of p21 translation determines cell fate.. The EMBO journal, (28): [PMID:14726956]
11. Hillman, R Tyler RT, Green, Richard E RE and Brenner, Steven E SE.. (2004) An unappreciated role for RNA surveillance.. Genome biology, [PMID:14759258]
12. Moraes, Karen C M KC, Wilusz, Carol J CJ and Wilusz, Jeffrey J.. (2006) CUG-BP binds to RNA substrates and recruits PARN deadenylase.. RNA (New York, N.Y.), [PMID:16601207]
13. Paul, Sharan S and 6 more authors.. (2006) Interaction of muscleblind, CUG-BP1 and hnRNP H proteins in DM1-associated aberrant IR splicing.. The EMBO journal, (20): [PMID:16946708]
14. Teplova, Marianna M, Song, Jikui J, Gaw, Hai Yan HY, Teplov, Alexei A and Patel, Dinshaw J DJ.. (2010) Structural insights into RNA recognition by the alternate-splicing regulator CUG-binding protein 1.. Structure (London, England : 1993), (13): [PMID:20947024]
15. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
16. Treiber, Thomas T and 9 more authors.. (2017) A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis.. Molecular cell, (20): [PMID:28431233]

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Recombinant CUG-BP1 Antibody - Primary antibody, specific to CUG-BP1, Rabbit IgG