Recombinant; Rabbit anti Human D4 GDI Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab099088-10μl | 10μl | In stock | $89.90 | |
| Ab099088-50μl | 50μl | In stock | $269.90 | |
| Ab099088-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $379.90 | |
| Ab099088-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Recombinant; Rabbit anti Human D4 GDI Antibody; WB; Unconjugated
| Product Name | Recombinant D4 GDI Antibody - Primary antibody, specific to ARHGDIB, Rabbit IgG |
|---|---|
| Synonyms | Ly-GDI|Rho GDI 2|Rho-GDI beta |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | ARHGDIB |
| Immunogen | A synthetic peptide derived from human D4 GDI (AA 176-189) |
| Positive Control | WB: Jurkat, U937 lysates |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human D4 GDI Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369). Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133). |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant D4 GDI Antibody (Ab099088) - Western Blot
All lanes: Recombinant D4 GDI Antibody (Ab099088) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 26 kDa
Exposure time: 30.0 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0506032 | Certificate of Analysis | May 23, 2024 | Ab099088 |
| ZJ24F0506031 | Certificate of Analysis | May 23, 2024 | Ab099088 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Aebersold, R R and Leavitt, J J.. (1990) Sequence analysis of proteins separated by polyacrylamide gel electrophoresis: towards an integrated protein database.. Electrophoresis, [PMID:2226408] |
| 3. Adra, C N CN and 5 more authors.. (1993) Identification of a novel protein with GDP dissociation inhibitor activity for the ras-like proteins CDC42Hs and rac I.. Genes, chromosomes & cancer, [PMID:7512369] |
| 4. Leffers, H H and 6 more authors.. (1993) Identification of two human Rho GDP dissociation inhibitor proteins whose overexpression leads to disruption of the actin cytoskeleton.. Experimental cell research, [PMID:8262133] |
| 5. Scherle, P P, Behrens, T T and Staudt, L M LM.. (1993) Ly-GDI, a GDP-dissociation inhibitor of the RhoA GTP-binding protein, is expressed preferentially in lymphocytes.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:8356058] |
| 6. Lelias, J M JM and 6 more authors.. (1993) cDNA cloning of a human mRNA preferentially expressed in hematopoietic cells and with homology to a GDP-dissociation inhibitor for the rho GTP-binding proteins.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:8434008] |
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| 8. Davis, A R AR, Clements, M K MK, Bunger, P L PL, Siemsen, D W DW and Quinn, M T MT.. (2000) Cloning and characterization of bovine low molecular weight GTPases (Rac1 and Rac2) and rho GDP-dissociation inhibitor 2 (D4-GDI).. Veterinary immunology and immunopathology, (23): [PMID:10802295] |
| 9. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455] |
| 10. Goshima, Naoki N and 54 more authors.. (2008) Human protein factory for converting the transcriptome into an in vitro-expressed proteome,.. Nature methods, [PMID:19054851] |
| 11. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 12. Boulter, Etienne E and 6 more authors.. (2010) Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1.. Nature cell biology, [PMID:20400958] |
