Recombinant; Rabbit anti Human Dihydrofolate reductase/DHFR Antibody; WB, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab099867-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab099867-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $189.90 | |
| Ab099867-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab099867-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,399.90 |
Recombinant; Rabbit anti Human Dihydrofolate reductase/DHFR Antibody; WB, ICC, IF; Unconjugated
| Product Name | Recombinant Dihydrofolate reductase/DHFR Antibody - Primary antibody, specific to Dihydrofolate reductase, Rabbit IgG |
|---|---|
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5 mg/mL |
| Host species | Rabbit |
| Specificity | Dihydrofolate reductase |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within human DHFR. |
| Positive Control | WB: A549, HeLa, THP-1, HEK293, Jurkat, NIH/3T3 and C6 cell lysates. ICC/IF: A375 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Dihydrofolate reductase/DHFR Antibody, Recombinant, could be used for WB, ICC/IF and so on. Application WB: 1/500-1/1000 ICC/IF: 1/50-1/200 Protein Function Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Immunogen affinity purified |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Dihydrofolate reductase/DHFR Antibody (Ab099867) - Western Blot
All lanes: Recombinant Dihydrofolate reductase/DHFR Antibody (Ab099867) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 23 kDa
Exposure time: 2.5 s
Recombinant Dihydrofolate reductase/DHFR Antibody (Ab099867) - IF/ICC
Immunofluorescent analysis of DHFR staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the Recombinant Dihydrofolate reductase/DHFR Antibody (Ab099867) in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin -AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| ICC/IF |
1/50-1/200
|
| WB |
1/500-1/1000
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0708400 | Certificate of Analysis | Jul 17, 2024 | Ab099867 |
| ZJ24F0708399 | Certificate of Analysis | Jul 17, 2024 | Ab099867 |
