Recombinant DNA Ligase IV/LIG4 Antibody

Features and benefits

Short Overview:
Recombinant; Rabbit anti Human DNA Ligase IV/LIG4 Antibody; WB, ICC, IF; Unconjugated
Species reactivity(Reacts with): Human
Isotype: Rabbit IgG

Application:

Item Number

Ab100073

Grouped product items
SKU	Size	Availability	Price
Ab100073-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$69.90
Ab100073-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$209.90
Ab100073-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$309.90
Ab100073-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,399.90

Recombinant; Rabbit anti Human DNA Ligase IV/LIG4 Antibody; WB, ICC, IF; Unconjugated

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Basic Description

Product Name	Recombinant DNA Ligase IV/LIG4 Antibody
Specifications & Purity	ExactAb™, Validated, KD, Recombinant, Lot by Lot
Host species	Rabbit
Specificity	LIG4
Immunogen	A synthetic peptide derived from human DNA Ligase IV (AA 504-685).
Positive Control	WB: MCF-7, HeLa, Jurkat, Ramos lysates. ICC/IF: HeLa cells.
Conjugation	Unconjugated
Grade	ExactAb™, KD Validation, Recombinant, Validated
Product Description	Rabbit anti Human DNA Ligase IV/LIG4 Antibody, Recombinant, could be used for WB, ICC, IF and so on. Application: WB: 1/500-1/2000 ICC/IF: 1/50-1/200 Protein Function: Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Product Properties

Isotype	Rabbit IgG
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Form	Liquid
Concentration	Lot by Lot
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Application

Dilution info

ICC/IF

1/50-1/200

1/500-1/2000

Solution Calculators

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Basic Description