Recombinant DYKDDDDK tag Antibody - Primary antibody, specific to DYKDDDDK, Rabbit IgG
Synonyms
DDDDK epitope tag antibody | DDDK antibody | ddk antibody | DYKDDDDK (also known as the FLAG® tag sequence) antibody | DYKDDDDK antibody | DYKDDDDK epitope tag antibody | DYKDDDDK tag antibody | ECS epitope tag antibody | ECS tag antibody | Enterokinase C
Specifications & Purity
ExactAb™, Validated, Recombinant, High performance, 0.7 mg/mL
Host species
Rabbit
Specificity
DYKDDDDK
Immunogen
A synthetic peptide of DDDDK tag (DYKDDDDK)
Positive Control
WB: HEK293 cells transfected with vector overexpressing DYKDDDDK tag.
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Recombinant, Validated
Product Description
Rabbit anti DYKDDDDK tag Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/1000 - 1/10000
Relevance
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.7 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant DYKDDDDK tag Antibody (Ab099358) - Western Blot All lanes: Recombinant DYKDDDDK tag Antibody (Ab099358) at 1/1000 dilution Lane 1: HEK293 Whole Cell Lysate Lane 2: HEK293 transfected with DYKDDDDK tag protein, Whole Cell Lysate
Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 27 kDa Observed band size: 28 kDa Exposure time: 6.1 s
Application
Application
Dilution info
WB
1/1000 - 1/10000
Certificates
Certificate of Analysis(COA)
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