A synthetic peptide derived from human DYNLL1 (AA 2-14)
Positive Control
WB: HeLa, Hep G2, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human colon cancer tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human DYNLL1/PIN Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures. Binds and inhibits the catalytic activity of neuronal nitric oxide synthase. Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1. Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant DYNLL1/PIN Antibody (Ab100668) - Western Blot All lanes: Recombinant DYNLL1/PIN Antibody (Ab100668) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 10 kDa Observed band size: 12 kDa Exposure time: 30.0 s
Recombinant DYNLL1/PIN Antibody (Ab100668) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant DYNLL1/PIN Antibody (Ab100668). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.