Recombinant; Rabbit anti Human eIF4B Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab101365-10μl | 10μl | In stock | $66.90 | |
| Ab101365-50μl | 50μl | In stock | $199.90 | |
| Ab101365-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $279.90 | |
| Ab101365-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human eIF4B Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant eIF4B Antibody - Primary antibody, specific to EIF4B, Rabbit IgG |
|---|---|
| Synonyms | Eif4a2 | eIF4B | EIF-4B | eukaryotic translation initiation factor 4B | PRO1843 | 2310046H11Rik antibody | AL024095 antibody | C85189 antibody | EIF 4B antibody | eIF-4B antibody | EIF4B antibody | Eukaryotic initiation factor 4B antibody | Eukaryotic tra |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.2 mg/mL |
| Host species | Rabbit |
| Specificity | EIF4B |
| Immunogen | A synthetic peptide derived from human eIF4B (AA 598-611) |
| Positive Control | WB: HeLa, U2OS and A549 cell lysates. IHC: Human lung cancer tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human eIF4B Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant eIF4B Antibody (Ab101365) - Western Blot
All lanes: Recombinant eIF4B Antibody (Ab101365) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 78 kDa
Exposure time: 10.0 s
Recombinant eIF4B Antibody (Ab101365) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant eIF4B Antibody (Ab101365). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303771 | Certificate of Analysis | Mar 27, 2024 | Ab101365 |
| ZJ24F0303770 | Certificate of Analysis | Mar 27, 2024 | Ab101365 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 3. Milburn, S C SC, Hershey, J W JW, Davies, M V MV, Kelleher, K K and Kaufman, R J RJ.. (1990) Cloning and expression of eukaryotic initiation factor 4B cDNA: sequence determination identifies a common RNA recognition motif.. The EMBO journal, [PMID:2390971] |
| 4. Méthot, N N, Pause, A A, Hershey, J W JW and Sonenberg, N N.. (1994) The translation initiation factor eIF-4B contains an RNA-binding region that is distinct and independent from its ribonucleoprotein consensus sequence.. Molecular and cellular biology, [PMID:8139536] |
| 5. Méthot, N N, Song, M S MS and Sonenberg, N N.. (1996) A region rich in aspartic acid, arginine, tyrosine, and glycine (DRYG) mediates eukaryotic initiation factor 4B (eIF4B) self-association and interaction with eIF3.. Molecular and cellular biology, [PMID:8816444] |
| 6. Raught, Brian B and 8 more authors.. (2004) Phosphorylation of eucaryotic translation initiation factor 4B Ser422 is modulated by S6 kinases.. The EMBO journal, (21): [PMID:15071500] |
| 7. Scherer, Steven E SE and 216 more authors.. (2006) The finished DNA sequence of human chromosome 12.. Nature, (16): [PMID:16541075] |
| 8. Shahbazian, David D and 9 more authors.. (2006) The mTOR/PI3K and MAPK pathways converge on eIF4B to control its phosphorylation and activity.. The EMBO journal, (21): [PMID:16763566] |
| 9. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243] |
| 10. Yu, Li-Rong LR and 5 more authors.. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.. Journal of proteome research, [PMID:17924679] |
| 11. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 12. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 13. Varela, Ignacio I and 43 more authors.. (2011) Exome sequencing identifies frequent mutation of the SWI/SNF complex gene PBRM1 in renal carcinoma.. Nature, (27): [PMID:21248752] |
| 14. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 15. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
