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Recombinant gamma Catenin Antibody - Primary antibody, specific to JUP, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 0.5 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human gamma Catenin Antibody; WB, IHC, IF, ICC; Unconjugated

  • Species reactivity(Reacts with): Human
  • Isotype: Rabbit IgG
    Application:
  • ICC/IF
  • IHC
  • WB
Item Number
Ab104679
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab104679-10μl
10μl
In stock
$69.90
Ab104679-50μl
50μl
In stock
$199.90
Ab104679-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$279.90
Ab104679-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human gamma Catenin Antibody; WB, IHC, IF, ICC; Unconjugated

Basic Description

Product NameRecombinant gamma Catenin Antibody - Primary antibody, specific to JUP, Rabbit IgG
SynonymsARVD 12 | ARVD12 | Catenin (cadherin associated protein) gamma 80kDa | Catenin (cadherin associated protein), gamma 80kDa | catenin (cadherin-associated protein) gamma (80kD) | Catenin gamma 80kDa | Catenin gamma | CTNNG | Desmoplakin 3 | Desmoplakin III
Specifications & PurityExactAb™, Validated, Recombinant, 0.5 mg/mL
Host speciesRabbit
SpecificityJUP
ImmunogenRecombinant protein of human gamma Catenin (AA 15-115)
Positive ControlWB: HeLa, A549, MCF7 and HEK293 cell lysates. IHC: Human Cholangiocarcinoma tissue. ICC/IF: HeLa cells
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human gamma Catenin Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.5 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant gamma Catenin Antibody (Ab104679) - Western Blot
All lanes: Recombinant gamma Catenin Antibody (Ab104679) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 82 kDa
Observed band size: 84 kDa
Exposure time: 65.6 s

Recombinant gamma Catenin Antibody (Ab104679) - IF/ICC
Immunocytochemistry analysis of gamma Catenin (green) in HeLa using Recombinant gamma Catenin Antibody (Ab104679), and cells were counterstained with DAPI (blue).

Recombinant gamma Catenin Antibody (Ab104679) - IHC
Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant gamma Catenin Antibody (Ab104679). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets

JUP Tbio Junction plakoglobin 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

ICC/IF

1/50-1/200

IHC

1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303316Certificate of AnalysisMar 15, 2024 Ab104679
ZJ24F0303317Certificate of AnalysisMar 15, 2024 Ab104679

Related Documents

References

1. Franke, W W WW and 5 more authors..  (1989)  Molecular cloning and amino acid sequence of human plakoglobin, the common junctional plaque protein..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2726765]
2. Yamamoto, M M, Bharti, A A, Li, Y Y and Kufe, D D..  (1997)  Interaction of the DF3/MUC1 breast carcinoma-associated antigen and beta-catenin in cell adhesion..  The Journal of biological chemistry,    (9):   [PMID:9139698]
3. McKoy, G G and 9 more authors..  (2000)  Identification of a deletion in plakoglobin in arrhythmogenic right ventricular cardiomyopathy with palmoplantar keratoderma and woolly hair (Naxos disease)..  Lancet (London, England),    (17):   [PMID:10902626]
4. Whittock, N V NV, Eady, R A RA and McGrath, J A JA..  (2000)  Genomic organization and amplification of the human plakoglobin gene (JUP)..  Experimental dermatology,      [PMID:11016852]
5. Chen, Xinyu X, Bonne, Stefan S, Hatzfeld, Mechthild M, van Roy, Frans F and Green, Kathleen J KJ..  (2002)  Protein binding and functional characterization of plakophilin 2. Evidence for its diverse roles in desmosomes and beta -catenin signaling..  The Journal of biological chemistry,    (22):   [PMID:11790773]
6. Holsinger, Leslie J LJ, Ward, Kevin K, Duffield, Bill B, Zachwieja, Joseph J and Jallal, Bahija B..  (2002)  The transmembrane receptor protein tyrosine phosphatase DEP1 interacts with p120(ctn)..  Oncogene,    (10):   [PMID:12370829]
7. Hatsell, Sarah S, Medina, Lillian L, Merola, Joe J, Haltiwanger, Robert R and Cowin, Pamela P..  (2003)  Plakoglobin is O-glycosylated close to the N-terminal destruction box..  The Journal of biological chemistry,    (26):   [PMID:12847106]
8. Miravet, Susana S and 6 more authors..  (2003)  Tyrosine phosphorylation of plakoglobin causes contrary effects on its association with desmosomes and adherens junction components and modulates beta-catenin-mediated transcription..  Molecular and cellular biology,      [PMID:14517306]
9. Zody, Michael C MC and 73 more authors..  (2006)  DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage..  Nature,    (20):   [PMID:16625196]
10. Asimaki, Angeliki A and 5 more authors..  (2007)  A novel dominant mutation in plakoglobin causes arrhythmogenic right ventricular cardiomyopathy..  American journal of human genetics,      [PMID:17924338]
11. Barahona-Dussault, C C and 7 more authors..  (2010)  Role of genetic testing in arrhythmogenic right ventricular cardiomyopathy/dysplasia..  Clinical genetics,      [PMID:19863551]
12. den Haan, A Dénise AD and 14 more authors..  (2009)  Comprehensive desmosome mutation analysis in north americans with arrhythmogenic right ventricular dysplasia/cardiomyopathy..  Circulation. Cardiovascular genetics,      [PMID:20031617]
13. Gehmlich, Katja K and 12 more authors..  (2011)  Mechanistic insights into arrhythmogenic right ventricular cardiomyopathy caused by desmocollin-2 mutations..  Cardiovascular research,    (1):   [PMID:21062920]
14. Bienvenut, Willy V WV and 6 more authors..  (2012)  Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features..  Molecular & cellular proteomics : MCP,      [PMID:22223895]
15. Kirchner, Florian F and 8 more authors..  (2012)  Molecular insights into arrhythmogenic right ventricular cardiomyopathy caused by plakophilin-2 missense mutations..  Circulation. Cardiovascular genetics,    (1):   [PMID:22781308]

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