Recombinant; Rabbit anti Human Gephyrin Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab105217-10μl | 10μl | In stock | $69.90 | |
| Ab105217-50μl | 50μl | In stock | $199.90 | |
| Ab105217-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $269.90 | |
| Ab105217-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human Gephyrin Antibody; WB; Unconjugated
| Product Name | Recombinant Gephyrin Antibody - Primary antibody, specific to Gephyrin, Rabbit IgG |
|---|---|
| Synonyms | HKPX1 | MOCODC | GEPH | Gephyrin | GPH | GPHN | GPHRYN | KIAA1385GPHGEPH | Domain E antibody | Domain G antibody | GEPH antibody | GEPH_HUMAN antibody | GPH antibody | GPHN antibody | GPHRYN antibody | KIAA1385 antibody | Molybdopterin molybdenumtransfera |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.4 mg/mL |
| Host species | Rabbit |
| Specificity | Gephyrin |
| Immunogen | A synthetic peptide derived from human Gephyrin (AA 1-54) |
| Positive Control | WB: HEK293, MCF7, Hep G2, U-2 OS, NIH/3T3 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human Gephyrin Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Gephyrin Antibody (Ab105217) - Western Blot
All lanes: Recombinant Gephyrin Antibody (Ab105217) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 94 kDa
Exposure time: 4.5 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505516 | Certificate of Analysis | May 14, 2024 | Ab105217 |
| ZJ24F0505515 | Certificate of Analysis | May 14, 2024 | Ab105217 |
| 1. Borislav Dejanovic,Marcus Semtner,Silvia Ebert,Tobias Lamkemeyer,Franziska Neuser,Bernhard Lüscher,Jochen C Meier,Guenter Schwarz. (2014-07-16) Palmitoylation of gephyrin controls receptor clustering and plasticity of GABAergic synapses.. PLoS biology, 12 ((7)): (e1001908-e1001908). [PMID:25025157] |
| 2. Nagase, T T, Kikuno, R R, Ishikawa, K I KI, Hirosawa, M M and Ohara, O O.. (2000) Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro.. DNA research : an international journal for rapid publication of reports on genes and genomes, (28): [PMID:10718198] |
| 3. Butler, M H MH and 7 more authors.. (2000) Autoimmunity to gephyrin in Stiff-Man syndrome.. Neuron, [PMID:10839351] |
| 4. Reiss, J J and 5 more authors.. (2001) A mutation in the gene for the neurotransmitter receptor-clustering protein gephyrin causes a novel form of molybdenum cofactor deficiency.. American journal of human genetics, [PMID:11095995] |
| 5. and David-Watine, B B.. (2001) The human gephyrin (GPHN) gene: structure, chromosome localization and expression in non-neuronal cells.. Gene, (27): [PMID:11418245] |
| 6. Schwarz, G G, Schrader, N N, Mendel, R R RR, Hecht, H J HJ and Schindelin, H H.. (2001) Crystal structures of human gephyrin and plant Cnx1 G domains: comparative analysis and functional implications.. Journal of molecular biology, (14): [PMID:11554796] |
| 7. Rees, Mark I MI and 15 more authors.. (2003) Isoform heterogeneity of the human gephyrin gene (GPHN), binding domains to the glycine receptor, and mutation analysis in hyperekplexia.. The Journal of biological chemistry, (4): [PMID:12684523] |
| 8. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243] |
| 9. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 10. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 11. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 12. Reiss, J J and 5 more authors.. (2011) A GPHN point mutation leading to molybdenum cofactor deficiency.. Clinical genetics, [PMID:22040219] |
| 13. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 14. Dejanovic, Borislav B and 18 more authors.. (2015) Simultaneous impairment of neuronal and metabolic function of mutated gephyrin in a patient with epileptic encephalopathy.. EMBO molecular medicine, [PMID:26613940] |
