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Recombinant GFP Antibody - Primary antibody, specific to GFP, Rabbit IgG

ExactAb™
High performance
Recombinant
Validated
0.2 mg/mL

Features and benefits

Short Overview:
Recombinant; Rabbit anti GFP Antibody; WB; Unconjugated
Species reactivity(Reacts with): Species independent
Isotype: Rabbit IgG

Application:

Item Number

Ab105277

Grouped product items
SKU	Size	Availability	Price
Ab105277-10μl	10μl	In stock	$66.90
Ab105277-50μl	50μl	In stock	$199.90
Ab105277-100μl	100μl	In stock	$299.90
Ab105277-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,999.90

Recombinant; Rabbit anti GFP Antibody; WB; Unconjugated

View related series

Accession#:P42212 GFP Tag

Basic Description

Product Name	Recombinant GFP Antibody - Primary antibody, specific to GFP, Rabbit IgG
Synonyms	GFP antibody; Green fluorescent protein antibody; eGFP; Enhanced Green Fluorescent Protein; GFP; GFPuv; green fluorescent protein (gfp); Green Fluorescent Protein; GFP Tag antibody; Anti- GFP Tag antibody
Specifications & Purity	ExactAb™, Validated, Recombinant, High performance, 0.2 mg/mL
Host species	Rabbit
Specificity	GFP
Immunogen	Recombinant protein of GFP (AA 1-238).
Positive Control	WB: 944-GFP lysates.
Conjugation	Unconjugated
Grade	ExactAb™, High performance, Recombinant, Validated
Product Description	Rabbit anti GFP Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/1000 - 1/10000 Relevance Function: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca 2+ -activated photoprotein aequorin. Subunit structure: Monomer. Tissue specificity: Photocytes. Post-translational modification: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. Biotechnological use: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy. Sequence similarities: Belongs to the GFP family. Biophysicochemical properties: Absorption: Abs(max)=395 nm Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	0.2 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant GFP Antibody (Ab105277) - Western Blot
All lanes: Recombinant GFP Antibody (Ab105277) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 2.3 s

Recombinant GFP Antibody (Ab105277) - Western Blot
All lanes: Recombinant GFP Antibody (Ab105277) at 1/10000 dilution
Samples: Recombinant GFP Protein (rp156636) at 20 ng per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 50.0 s

Application

Dilution info

1/1000 - 1/10000

Certificates

Certificate of Analysis(COA)

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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0202984	Certificate of Analysis	Mar 01, 2024	Ab105277
ZJ23F1101546	Certificate of Analysis	Nov 02, 2023	Ab105277
ZJ23F1101545	Certificate of Analysis	Nov 02, 2023	Ab105277

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