Recombinant GRAF Antibody - Primary antibody, specific to GRAF, Rabbit IgG
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.5 mg/mL
Host species
Rabbit
Specificity
GRAF
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within human GRAF.
Positive Control
WB: HeLa, HEK293, Hep G2, NIH/3T3 and C6 cell lysates. IHC: Human ovary tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human GRAF Antibody, Recombinant , could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/200 Protein Function: GTPase-activating protein for RHOA and CDC42.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Immunogen affinity purified
Form
Liquid
Concentration
0.5 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant GRAF Antibody (Ab106304) - Western Blot All lanes: Recombinant GRAF Antibody (Ab106304) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 92 kDa Observed band size: 100 kDa Exposure time: 100.0 s
Recombinant GRAF Antibody (Ab106304) - Western Blot All lanes: Recombinant GRAF Antibody (Ab106304) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 92 kDa Observed band size: 100 kDa Exposure time: 50.0 s
Recombinant GRAF Antibody (Ab106304) - IF/ICC Immunohistochemical analysis of ARHGAP26 staining in human ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Recombinant GRAF Antibody (Ab106304) at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).
Associated Targets
ARHGAP26Tbio Rho GTPase-activating protein 26 0 Activities