Recombinant (RR673); Rabbit anti HA tag Antibody; WB, IF, ICC, Flow, IP; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab106771-10μl | 10μl | In stock | $66.90 | |
| Ab106771-50μl | 50μl | In stock | $199.90 | |
| Ab106771-100μl | 100μl | In stock | $299.90 | |
| Ab106771-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,199.90 |
Recombinant (RR673); Rabbit anti HA tag Antibody; WB, IF, ICC, Flow, IP; Unconjugated
| Product Name | Recombinant HA tag Antibody - Primary antibody, specific to HA, Rabbit IgG |
|---|---|
| Synonyms | HA epitope tag antibody; HA1 antibody; HA2 antibody; hemagglutinin antibody; Hemagglutinin HA1 chain antibody; Hemagglutinin HA2 chain antibody; HA Epitope Tag; HA Tag; HA; Hemagglutinin; HA tag antibody; Anti- HA tag antibody |
| Specifications & Purity | Recombinant, ExactAb™, Validated, High performance, 1.0 mg/mL |
| Host species | Rabbit |
| Specificity | HA |
| Immunogen | YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH. |
| Positive Control | WB: Recombinant Human HA-Ubiquitin Protein, HEK293 cells transfected with HA tag. IF/ICC: 293 cells transfected with N-terminal and C-terminal HA tagged gene. Flow: 293 cells transfected with N-terminal and C-terminal HA tagged gene. IP: 293 whole cell ly |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Recombinant, Validated |
| Product Description |
Rabbit anti HA tag Antibody, Recombinant (RR673), could be used for WB, IF, ICC, Flow, IP and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant HA tag Antibody (Ab106771) - Western Blot
All lanes: Recombinant HA tag Antibody (Ab106771) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 64, 120 kDa
Exposure time: 8.8 s
Recombinant HA tag Antibody (Ab106771) - Western Blot
All lanes: Recombinant HA tag Antibody (Ab106771) at 1/5000 dilution
Samples: Recombinant Human HA-Ubiquitin Protein (rp170435) at 20 ng per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution
Predicted band size: 11 kDa
Observed band size: 10 kDa
Exposure time: 2.3 s
Recombinant HA tag Antibody (Ab106771) - IF/ICC
Recombinant HA tag Antibody (Ab106771) staining HA tag in 293 cells transfected with N-terminal HA tagged gene by IF/ICC
(immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant HA tag Antibody (Ab106771) (1/2000) at 4°C. An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant HA tag Antibody (Ab106771) - IF/ICC
Recombinant HA tag Antibody (Ab106771) staining HA tag in 293 cells transfected with C-terminal HA tagged gene by IF/ICC(immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant HA tag Antibody (Ab106771) (1/2000) at 4°C. An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant HA tag Antibody (Ab106771) - Flow Cytometry
Overlay histogram showing 293 cells transfected with N-terminal (Green) and C-terminal (Red) HA tagged gene stained with Recombinant HA tag Antibody (Ab106771). The cells were then incubated in the Recombinant HA tag Antibody (Ab106771) (1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
Recombinant HA tag Antibody (Ab106771) - IP
HA tag was immunoprecipitated from 0.2 mg of 293 whole cells lysate transfected with N-terminal HA tagged gene with Recombinant HA tag Antibody (Ab106771) at 1/10 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant HA tag Antibody (Ab106771) IP in 293 whole cell lysate transfected with N-terminal HA tagged gene
Lane 2: PBS instead of Recombinant HA tag Antibody (Ab106771) in 293 whole cell lysate transfected with N-terminal HA tagged gene
Lane 3: 293 whole cell lysate transfected with N-terminal HA tagged gene, 10 μg (input).
Recombinant HA tag Antibody (Ab106771) - IP
HA tag was immunoprecipitated from 0.2 mg of 293 whole cells lysate transfected with C-terminal HA tagged gene with Recombinant HA tag Antibody (Ab106771) at 1/10 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant HA tag Antibody (Ab106771) IP in 293 whole cell lysate transfected with C-terminal HA tagged gene
Lane 2: PBS instead of Recombinant HA tag Antibody (Ab106771) in 293 whole cell lysate transfected with C-terminal HA tagged gene
Lane 3: 293 whole cell lysate transfected with C-terminal HA tagged gene, 10 μg (input).
| Application | Dilution info |
|---|---|
| IF/ICC | 1/400 - 1/2000 |
| Flow Cytometry | 1/400 - 1/2000 |
| IP | 1/10 |
| WB | 1/1000 - 1/5000 |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404377 | Certificate of Analysis | Apr 10, 2024 | Ab106771 |
| ZJ24F0404376 | Certificate of Analysis | Apr 10, 2024 | Ab106771 |
| ZJ24F0404375 | Certificate of Analysis | Apr 10, 2024 | Ab106771 |
