Recombinant HA tag Antibody - Primary antibody, specific to HA, Rabbit IgG

    Application:
  • Flow Cytometry
  • IF/ICC
  • IP
  • WB
Features and benefits
  • Host species: Rabbit
  • Species reactivity(Reacts with): Species independent
  • Clone Number: RR673
  • Conjugation: Unconjugated
Item Number
Ab106771
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab106771-10μl
10μl
In stock
$66.90
Ab106771-50μl
50μl
In stock
$199.90
Ab106771-100μl
100μl
In stock
$299.90
Ab106771-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,199.90

Recombinant (RR673); Rabbit anti HA tag Antibody; WB, IF, ICC, Flow, IP; Unconjugated

View related series
HA tag Tag

Basic Description

Product NameRecombinant HA tag Antibody - Primary antibody, specific to HA, Rabbit IgG
SynonymsHA epitope tag antibody | HA1 antibody | HA2 antibody | hemagglutinin antibody | Hemagglutinin HA1 chain antibody | Hemagglutinin HA2 chain antibody | HA Epitope Tag | HA Tag | HA | Hemagglutinin | HA tag antibody | Anti- HA tag antibody
Specifications & PurityRecombinant, ExactAb™, Validated, High performance, 1.0 mg/mL
Host speciesRabbit
SpecificityHA
ImmunogenYPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH.
Positive ControlWB: Recombinant Human HA-Ubiquitin Protein, HEK293 cells transfected with HA tag. IF/ICC: 293 cells transfected with N-terminal and C-terminal HA tagged gene. Flow: 293 cells transfected with N-terminal and C-terminal HA tagged gene. IP: 293 whole cell ly
ConjugationUnconjugated
GradeExactAb™, High Performance, Recombinant, Validated
Product Description

Rabbit anti HA tag Antibody, Recombinant (RR673), could be used for WB, IF, ICC, Flow, IP and so on.
Application:
WB: 1/1000 - 1/5000
IF/ICC: 1/400 - 1/2000
Flow: 1/400 - 1/2000
IP: 1/10
Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodProtein A purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant HA tag Antibody (Ab106771) - Western Blot
All lanes: Recombinant HA tag Antibody (Ab106771) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 64, 120 kDa
Exposure time: 8.8 s

Recombinant HA tag Antibody (Ab106771) - Western Blot
All lanes: Recombinant HA tag Antibody (Ab106771) at 1/5000 dilution
Samples: Recombinant Human HA-Ubiquitin Protein (rp170435) at 20 ng per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution
Predicted band size: 11 kDa
Observed band size: 10 kDa
Exposure time: 2.3 s

Recombinant HA tag Antibody (Ab106771) - IF/ICC
Recombinant HA tag Antibody (Ab106771) staining HA tag in 293 cells transfected with N-terminal HA tagged gene by IF/ICC
(immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant HA tag Antibody (Ab106771) (1/2000) at 4°C. An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG (1/500).

Recombinant HA tag Antibody (Ab106771) - IF/ICC
Recombinant HA tag Antibody (Ab106771) staining HA tag in 293 cells transfected with C-terminal HA tagged gene by IF/ICC(immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant HA tag Antibody (Ab106771) (1/2000) at 4°C. An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG (1/500).

Recombinant HA tag Antibody (Ab106771) - Flow Cytometry
Overlay histogram showing 293 cells transfected with N-terminal (Green) and C-terminal (Red) HA tagged gene stained with Recombinant HA tag Antibody (Ab106771). The cells were then incubated in the Recombinant HA tag Antibody (Ab106771) (1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

Recombinant HA tag Antibody (Ab106771) - IP
HA tag was immunoprecipitated from 0.2 mg of 293 whole cells lysate transfected with N-terminal HA tagged gene with Recombinant HA tag Antibody (Ab106771) at 1/10 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant HA tag Antibody (Ab106771) IP in 293 whole cell lysate transfected with N-terminal HA tagged gene
Lane 2: PBS instead of Recombinant HA tag Antibody (Ab106771) in 293 whole cell lysate transfected with N-terminal HA tagged gene
Lane 3: 293 whole cell lysate transfected with N-terminal HA tagged gene, 10 μg (input).

Recombinant HA tag Antibody (Ab106771) - IP
HA tag was immunoprecipitated from 0.2 mg of 293 whole cells lysate transfected with C-terminal HA tagged gene with Recombinant HA tag Antibody (Ab106771) at 1/10 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant HA tag Antibody (Ab106771) IP in 293 whole cell lysate transfected with C-terminal HA tagged gene
Lane 2: PBS instead of Recombinant HA tag Antibody (Ab106771) in 293 whole cell lysate transfected with C-terminal HA tagged gene
Lane 3: 293 whole cell lysate transfected with C-terminal HA tagged gene, 10 μg (input).

Application

ApplicationDilution info
IF/ICC

1/400 - 1/2000

Flow Cytometry

1/400 - 1/2000

IP

1/10

WB1/1000 - 1/5000

">

1/1000 - 1/5000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404377Certificate of AnalysisApr 10, 2024 Ab106771
ZJ24F0404376Certificate of AnalysisApr 10, 2024 Ab106771
ZJ24F0404375Certificate of AnalysisApr 10, 2024 Ab106771

Related Documents

Solution Calculators