Recombinant HA tag Antibody - Primary antibody, specific to HA, Rabbit IgG
Synonyms
HA epitope tag antibody | HA1 antibody | HA2 antibody | hemagglutinin antibody | Hemagglutinin HA1 chain antibody | Hemagglutinin HA2 chain antibody | HA Epitope Tag | HA Tag | HA | Hemagglutinin | HA tag antibody | Anti- HA tag antibody
Specifications & Purity
Recombinant, ExactAb™, Validated, High performance, 1.0 mg/mL
Host species
Rabbit
Specificity
HA
Immunogen
YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH.
Positive Control
WB: Recombinant Human HA-Ubiquitin Protein, HEK293 cells transfected with HA tag. IF/ICC: 293 cells transfected with N-terminal and C-terminal HA tagged gene. Flow: 293 cells transfected with N-terminal and C-terminal HA tagged gene. IP: 293 whole cell ly
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Recombinant, Validated
Product Description
Rabbit anti HA tag Antibody, Recombinant (RR673), could be used for WB, IF, ICC, Flow, IP and so on. Application: WB: 1/1000 - 1/5000 IF/ICC: 1/400 - 1/2000 Flow: 1/400 - 1/2000 IP: 1/10 Relevance Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant HA tag Antibody (Ab106771) - Western Blot All lanes: Recombinant HA tag Antibody (Ab106771) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 115 kDa Observed band size: 64, 120 kDa Exposure time: 8.8 s
Recombinant HA tag Antibody (Ab106771) - Western Blot All lanes: Recombinant HA tag Antibody (Ab106771) at 1/5000 dilution Samples: Recombinant Human HA-Ubiquitin Protein (rp170435) at 20 ng per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution Predicted band size: 11 kDa Observed band size: 10 kDa Exposure time: 2.3 s
Recombinant HA tag Antibody (Ab106771) - IF/ICC Recombinant HA tag Antibody (Ab106771) staining HA tag in 293 cells transfected with N-terminal HA tagged gene by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant HA tag Antibody (Ab106771) (1/2000) at 4°C. An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant HA tag Antibody (Ab106771) - IF/ICC Recombinant HA tag Antibody (Ab106771) staining HA tag in 293 cells transfected with C-terminal HA tagged gene by IF/ICC(immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant HA tag Antibody (Ab106771) (1/2000) at 4°C. An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 594-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant HA tag Antibody (Ab106771) - Flow Cytometry Overlay histogram showing 293 cells transfected with N-terminal (Green) and C-terminal (Red) HA tagged gene stained with Recombinant HA tag Antibody (Ab106771). The cells were then incubated in the Recombinant HA tag Antibody (Ab106771) (1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
Recombinant HA tag Antibody (Ab106771) - IP HA tag was immunoprecipitated from 0.2 mg of 293 whole cells lysate transfected with N-terminal HA tagged gene with Recombinant HA tag Antibody (Ab106771) at 1/10 dilution. 2nd Ab: GAR HRP for IP 1/500 Lane 1: Recombinant HA tag Antibody (Ab106771) IP in 293 whole cell lysate transfected with N-terminal HA tagged gene Lane 2: PBS instead of Recombinant HA tag Antibody (Ab106771) in 293 whole cell lysate transfected with N-terminal HA tagged gene Lane 3: 293 whole cell lysate transfected with N-terminal HA tagged gene, 10 μg (input).
Recombinant HA tag Antibody (Ab106771) - IP HA tag was immunoprecipitated from 0.2 mg of 293 whole cells lysate transfected with C-terminal HA tagged gene with Recombinant HA tag Antibody (Ab106771) at 1/10 dilution. 2nd Ab: GAR HRP for IP 1/500 Lane 1: Recombinant HA tag Antibody (Ab106771) IP in 293 whole cell lysate transfected with C-terminal HA tagged gene Lane 2: PBS instead of Recombinant HA tag Antibody (Ab106771) in 293 whole cell lysate transfected with C-terminal HA tagged gene Lane 3: 293 whole cell lysate transfected with C-terminal HA tagged gene, 10 μg (input).
Application
Application
Dilution info
IF/ICC
1/400 - 1/2000
Flow Cytometry
1/400 - 1/2000
IP
1/10
WB
1/1000 - 1/5000 ">
1/1000 - 1/5000
Certificates
Certificate of Analysis(COA)
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