Recombinant His tag Antibody - Primary antibody, specific to 6X His, Rabbit IgG
Synonyms
6 His epitope tag antibody; Hexa His tag antibody; HHHHHH epitope tag antibody; HHHHHH tag antibody; His tag antibody; Polyhistidine Tag antibody; Histidine Tag; 6 His epitope tag; 6-His Tag; 6X His Tag ; 6X His; 6x-His Tag; 6X-His; H; Hexa His tag; HHHHH
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.5mg/mL
Host species
Rabbit
Specificity
6X His
Immunogen
Synthetic peptide: HHHHHH (6X His) conjugated to KLH.
Positive Control
WB: Fusion protein with N terminal and C terminal 6X His tag; Multi tag fusion protein containing 6X His tag; 293 cells lysate transfected with N terminal and C terminal 6X His tagged gene. IF/ICC: 293 cells trasnfected with N terminal and C terminal 6X H
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti His tag Antibody, Recombinant (RR689), could be used for WB, IF, ICC, Flow, IP and so on. Application: WB: 1/1000 - 1/2000 IF/ICC: 1/800 - 1/2000 Flow: 1/200 - 1/1000 IP: 1/50 Relevance The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
0.5mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Application
Application
Dilution info
WB
1/1000 - 1/2000
IF/ICC
1/800 - 1/2000
IP
1/50
Flow Cytometry
1/200 - 1/1000
Certificates
Certificate of Analysis(COA)
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
ZJ24F0506205