| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab086830-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $66.90 | |
| Ab086830-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab086830-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $329.90 | |
| Ab086830-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
Recombinant (RR689); Rabbit anti His tag Antibody; WB, IF, ICC, Flow, IP; Unconjugated
| Product Name | Recombinant His tag Antibody - Primary antibody, specific to 6X His, Rabbit IgG |
|---|---|
| Synonyms | 6 His epitope tag antibody | Hexa His tag antibody | HHHHHH epitope tag antibody | HHHHHH tag antibody | His tag antibody | Polyhistidine Tag antibody | Histidine Tag | 6 His epitope tag | 6-His Tag | 6X His Tag | 6X His | 6x-His Tag | 6X-His | H | Hexa H |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.5mg/mL |
| Host species | Rabbit |
| Specificity | 6X His |
| Immunogen | Synthetic peptide: HHHHHH (6X His) conjugated to KLH. |
| Positive Control | WB: His transfected HEK293 cell lysate. IF/ICC: HEK293 cells trasnfected with N terminal and C terminal 6X His tagged gene. Flow: HEK293 cells transfected with N terminal and C terminal 6X His tagged gene. IP: HEK293 cells transfected with N terminal and |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti His tag Antibody, Recombinant (RR689), could be used for WB, IF, ICC, Flow, IP and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.5mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant His tag Antibody (Ab086830) - Western Blot
All lanes: Recombinant His tag Antibody (Ab086830) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 116 kDa
Observed band size: 47, 55,112 kDa
Exposure time: 0.3 s
Recombinant His tag Antibody(Ab086830) - IF/ICC
Recombinant His tag Antibody (Ab086830) staining HEK293 cells trasnfected with N-terminal 6X His tagged gene by immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant His tag Antibody (Ab086830) (1/10000) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant His tag Antibody(Ab086830) - IF/ICC
Recombinant His tag Antibody (Ab086830) staining HEK293 cells trasnfected with C-terminal 6X His tagged gene by immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant His tag Antibody (Ab086830) (1/800) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (1/500).
Recombinant His tag Antibody(Ab086830) - IP
His tag was immunoprecipitated from 0.05 mg of HEK293 whole cells lysate transfected with N-terminal 6X His tagged gene with Recombinant His tag Antibody (Ab086830) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant His tag Antibody(Ab086830) IP in HEK293 whole cell lysate transfected with N-terminal 6X His tagged gene
Lane 2: PBS instead of Recombinant His tag Antibody(Ab086830) in HEK293 whole cell lysate transfected with N-terminal 6X His tagged gene
Lane 3: HEK293 whole cell lysate transfected with N-terminal 6X His tagged gene, 10 μg (input)
Exposure: 20.0s
Recombinant His tag Antibody(Ab086830) - IP
His tag was immunoprecipitated from 0.2 mg of HEK293 whole cells lysate transfected with C-terminal 6X His tagged gene with Recombinant His tag Antibody(Ab086830) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant His tag Antibody(Ab086830) IP in HEK293 whole cells lysate transfected with C-terminal 6X His tagged gene
Lane 2: PBS instead of Recombinant His tag Antibody(Ab086830) in HEK293 whole cells lysate transfected with C-terminal 6X His tagged gene
Lane 3: HEK293 whole cells lysate transfected with C-terminal 6X His tagged gene, 10 μg (input)
Exposure: 120.0s
Recombinant His tag Antibody(Ab086830) - Flow Cytometry
Overlay histogram showing HEK293 cells transfected with N-terminal (Blue) and C-terminal (Red) 6X His tagged gene stained with Recombinant His tag Antibody (Ab086830). The cells were then incubated in the antibody (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
| Application | Dilution info |
|---|---|
| WB | 1/1000 - 1/2000"> 1/1000 - 1/2000 |
| IF/ICC | 1/800 - 1/2000 |
| IP |
1/50
|
| Flow Cytometry |
1/200 - 1/1000
|
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0506205 | Certificate of Analysis | May 28, 2024 | Ab086830 |
| ZJ24F0506204 | Certificate of Analysis | May 28, 2024 | Ab086830 |
| ZJ24F0506203 | Certificate of Analysis | May 28, 2024 | Ab086830 |
