A synthetic peptide derived from human Histone H3.3 (AA 70-100).
Positive Control
WB: K562 cell lysate. IHC: Mouse stomach tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human Histone H3.3 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/500-1/1000 Protein Function: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
Lot by Lot
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Histone H3.3 Antibody (Ab108010) - Western Blot All lanes: Recombinant Histone H3.3 Antibody (Ab108010) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 15 kDa Observed band size: 17 kDa Exposure time: 10.0 s
Recombinant Histone H3.3 Antibody (Ab108010) - IHC Immunohistochemical analysis of paraffin-embedded mouse stomach, using Recombinant Histone H3.3 Antibody (Ab108010).
Application
Application
Dilution info
WB
1/500-1/1000
IHC
1/500-1/1000
Certificates
Certificate of Analysis(COA)
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