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Recombinant hnRNP Q Antibody - Primary antibody, specific to SYNCRIP, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 0.8 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human hnRNP Q Antibody; WB, IHC, IF, ICC; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • IF/ICC
  • IHC
  • WB
Item Number
Ab108467
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab108467-10μl
10μl
In stock
$69.90
Ab108467-50μl
50μl
In stock
$199.90
Ab108467-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab108467-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

Recombinant; Rabbit anti Human hnRNP Q Antibody; WB, IHC, IF, ICC; Unconjugated

Basic Description

Product NameRecombinant hnRNP Q Antibody - Primary antibody, specific to SYNCRIP, Rabbit IgG
SynonymsGlycine- and tyrosine-rich RNA-binding protein | GRY-RBP | hnRNP Q | NS1-associated protein 1 | Synaptotagmin-binding, cytoplasmic RNA-interacting protein | cytoplasmic RNA-interacting protein | dJ3J17.2 | Glycine and tyrosine rich RNA binding protein | G
Specifications & PurityExactAb™, Validated, Recombinant, 0.8 mg/mL
Host speciesRabbit
SpecificitySYNCRIP
ImmunogenA synthetic peptide derived from human hnRNP Q (AA 603-616)
Positive ControlWB: K562, Hep G2 and C6 cell lysates. IHC: Human tonsil tissue. ICC/IF: HeLa cells
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human hnRNP Q Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.
Application:
WB: 1/500-1/2000
IHC: 1/50-1/100
ICC/IF: 1/50-1/200
Protein Function
Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins.






Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.8 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant hnRNP Q Antibody (Ab108467) - Western Blot
All lanes: Recombinant hnRNP Q Antibody (Ab108467) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 70 kDa
Observed band size: 72 kDa
Exposure time: 8.5 s

Recombinant hnRNP Q Antibody (Ab108467) - Western Blot
All lanes: Recombinant hnRNP Q Antibody (Ab108467) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 70 kDa
Observed band size: 69 kDa
Exposure time: 25.0 s

Recombinant hnRNP Q Antibody (Ab108467) - IF/ICC
Immunocytochemistry analysis of hnRNP Q (green) in HeLa using Recombinant hnRNP Q Antibody (Ab108467), and cells were counterstained with DAPI (blue).

Recombinant hnRNP Q Antibody (Ab108467) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant hnRNP Q Antibody (Ab108467). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets

SYNCRIP Tbio Heterogeneous nuclear ribonucleoprotein Q 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/2000

ICC/IF

1/50-1/200

IHC

1/50 - 1/100

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404054Certificate of AnalysisApr 02, 2024 Ab108467
ZJ24F0303886Certificate of AnalysisApr 02, 2024 Ab108467

Related Documents

References

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10. Carty, Sherry M SM and Greenleaf, Arno L AL..  (2002)  Hyperphosphorylated C-terminal repeat domain-associating proteins in the nuclear proteome link transcription to DNA/chromatin modification and RNA processing..  Molecular & cellular proteomics : MCP,      [PMID:12376575]
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13. Passos, Dario O DO, Quaresma, Alexandre J C AJ and Kobarg, Jörg J..  (2006)  The methylation of the C-terminal region of hnRNPQ (NSAP1) is important for its nuclear localization..  Biochemical and biophysical research communications,    (28):   [PMID:16765914]
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17. Bressan, Gustavo C GC and 6 more authors..  (2009)  Functional association of human Ki-1/57 with pre-mRNA splicing events..  The FEBS journal,      [PMID:19523114]
18. Arif, Abul A and 5 more authors..  (2009)  Two-site phosphorylation of EPRS coordinates multimodal regulation of noncanonical translational control activity..  Molecular cell,    (31):   [PMID:19647514]
19. Arif, Abul A, Chatterjee, Piyali P, Moodt, Robyn A RA and Fox, Paul L PL..  (2012)  Heterotrimeric GAIT complex drives transcript-selective translation inhibition in murine macrophages..  Molecular and cellular biology,      [PMID:23071094]
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