Recombinant HSPA2 Antibody - Primary antibody, specific to HSPA2, Rabbit IgG
Synonyms
Heat shock 70 kDa protein 2 | Heat shock 70 kDa protein 2 | heat shock 70kD protein 2 | heat shock 70kDa protein 2 | heat shock-related 70 kDa protein 2 | HSP70-2 | HSP70-3 | HSPA2 | 70kDa antibody | Hcp70.2 antibody | Heat shock 70 kDa protein 2 antibody
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.8 mg/mL
Host species
Rabbit
Specificity
HSPA2
Immunogen
Recombinant protein of human HSPA2 (AA 488-637)
Positive Control
WB: A549, A431, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human colon cancer tissue.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human HSPA2 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
Product Properties
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Form
Liquid
Concentration
0.8 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant HSPA2 Antibody (Ab108960) - Western Blot All lanes: Recombinant HSPA2 Antibody (Ab108960) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 70 kDa Observed band size: 71 kDa Exposure time: 29.1 s
Recombinant HSPA2 Antibody (Ab108960) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant HSPA2 Antibody (Ab108960). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Associated Targets
HSPA2Tchem Heat shock-related 70 kDa protein 2 0 Activities
1.Radons J. (2016) The human HSP70 family of chaperones: where do we stand?. Cell Stress Chaperones, 21 (3):(379-404). [PMID:26865365]
2.Wang RE, Hunt CR, Chen J, Taylor JS.. (2011) Biotinylated quercetin as an intrinsic photoaffinity proteomics probe for the identification of quercetin target proteins.. Bioorg Med Chem, 19 (16):(4710-4720). [PMID:21798748]
3.Bonnycastle, L L LL and 7 more authors.. (1994) Cloning, sequencing, and mapping of the human chromosome 14 heat shock protein gene (HSPA2).. Genomics, (1):[PMID:7829106]
4.Roux, A F AF, Nguyen, V T VT, Squire, J A JA and Cox, D W DW.. (1994) A heat shock gene at 14q22: mapping and expression.. Human molecular genetics, [PMID:7849706]
5.Jarczowski, Franziska F, Fischer, Gunter G and Edlich, Frank F.. (2008) FKBP36 forms complexes with clathrin and Hsp72 in spermatocytes.. Biochemistry, (1):[PMID:18529014]
6.Wisniewska, Magdalena and 6 more authors.. (2010) Crystal structures of the ATPase domains of four human Hsp70 isoforms: HSPA1L/Hsp70-hom, HSPA2/Hsp70-2, HSPA6/Hsp70B', and HSPA5/BiP/GRP78.. PloS one, (11):[PMID:20072699]
7.Jakobsson, Magnus E ME and 6 more authors.. (2013) Identification and characterization of a novel human methyltransferase modulating Hsp70 protein function through lysine methylation.. The Journal of biological chemistry, (27):[PMID:23921388]
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