Recombinant HSPA4 Antibody - Primary antibody, specific to HSPA4, Rabbit IgG
Synonyms
Heat shock 70-related protein APG-2 | HSP70RY | APG 2 antibody | Epididymis secretory sperm binding protein Li 5a antibody | Heat shock 70 kDa protein 4 antibody | Heat shock 70 related protein APG 2 antibody | Heat shock 70-related protein APG-2 antibody
Specifications & Purity
ExactAb™, Validated, Recombinant, 1.17 mg/mL
Host species
Rabbit
Specificity
HSPA4
Immunogen
A synthetic peptide derived from human HSPA4 (AA 700-750)
Positive Control
WB: Hep G2, K562, HeLa, MCF7 and HEK293 cell lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Product Description
Rabbit anti Human HSPA4 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Post-translational
Phosphorylated upon DNA damage, probably by ATM or ATR.
Product Properties
Isotype
Rabbit IgG
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Form
Liquid
Concentration
1.17 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant HSPA4 Antibody (Ab108963) - Western Blot All lanes: Recombinant HSPA4 Antibody (Ab108963) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 94 kDa Observed band size: 107 kDa Exposure time: 32.7 s
Associated Targets
HSPA4Tbio Heat shock 70 kDa protein 4 0 Activities
1.Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942):(834-40). [PMID:19608861]
2.Fathallah, D M DM, Cherif, D D, Dellagi, K K and Arnaout, M A MA.. (1993) Molecular cloning of a novel human hsp70 from a B cell line and its assignment to chromosome 5.. Journal of immunology (Baltimore, Md. : 1950), (15):[PMID:8335910]
3.Schmutz, Jeremy J and 75 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 5.. Nature, (16):[PMID:15372022]
4.Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, [PMID:15592455]
5.Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25):[PMID:17525332]
6.Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5):[PMID:18669648]
7.Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18):[PMID:19690332]
8.Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15):[PMID:21406692]
9.Mi, Lixin and 8 more authors.. (2011) Identification of potential protein targets of isothiocyanates by proteomics.. Chemical research in toxicology, (17):[PMID:21838287]
10.Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4):[PMID:23186163]
11.Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315]
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