Recombinant Human 2B4/CD244/SLAMF4 Protein, ≥95% (SDS-PAGE), high purity

Features and benefits
  • Expression System: HEK293
  • Accession #: Q9BZW8
  • Protein Tag: C-hFc & His
  • Bioactivity: Immobilized recombinant human CD48 at 5 μg/mL (100 μL/well) can bind recombinant human CD244 with a linear range of 0.2-1 μg/mL.
  • Endotoxin Concentration: <0.1 EU/μg
Item Number
rp142380
Grouped product items
SKUSizeAvailabilityPrice Qty
rp142380-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$139.90
rp142380-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$419.90
rp142380-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$659.90
rp142380-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$4,999.90

Animal Free, ≥95% (SDS-PAGE), Active, 293F, C-hFc&His tag, 22-221 aa

Basic Description

Product NameRecombinant Human 2B4/CD244/SLAMF4 Protein, ≥95% (SDS-PAGE), high purity
Synonymsh2B4 | NAIL | NK cell activation-inducing ligand | NK cell type I receptor protein 2B4 | NKR2B4 | Signaling lymphocytic activation molecule 4 | SLAM family member 4 | SLAMF4 | 2B4 | CD244 antigen | CD244 molecule, natural killer cell receptor 2B4 | CD244
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description

Purity
≥95% SDS-PAGE.
Endotoxin level
<0.1 EU/µg
Function
Modulate other receptor-ligand interactions to enhance leukocyte activation.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
Purity≥95% (SDS-PAGE)
BioactivityImmobilized recombinant human CD48 at 5 μg/mL (100 μL/well) can bind recombinant human CD244 with a linear range of 0.2-1 μg/mL.
Endotoxin Concentration<0.1 EU/μg
Expression SystemHEK293
SpeciesHuman
Amino Acids22-221 aa
SequenceCQGSADHVVSISGVPLQLQPNSIQTKVDSIAWKKLLPSQNGFHHILKWENGSLPSNTSNDRFSFIVKNLSLLIKAAQQQDSGLYCLEVTSISGKVQTATFQVFVFDKVEKPRLQGQGKILDRGRCQVALSCLVSRDGNVSYAWYRGSKLIQTAGNLTYLDEEVDINGTHTYTCNVSNPVSWESHTLNLTQDCQNAHQEFRENLYFQGMDPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCV
Protein TagC-hFc & His
Accession #Q9BZW8
Predicted molecular weight50 kDa
SDS-PAGE70-90 kDa under reducing conditions

Product Specifications

FormLyophilized
ReconstitutionReconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Related Documents

References

1. Valiante, N M NM and Trinchieri, G G..  (1993)  Identification of a novel signal transduction surface molecule on human cytotoxic lymphocytes..  The Journal of experimental medicine,    (1):   [PMID:8376943]
2. Brown, M H MH and 5 more authors..  (1998)  2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48..  The Journal of experimental medicine,    (7):   [PMID:9841922]
3. Tangye, S G SG and 5 more authors..  (1999)  Cutting edge: human 2B4, an activating NK cell receptor, recruits the protein tyrosine phosphatase SHP-2 and the adaptor signaling protein SAP..  Journal of immunology (Baltimore, Md. : 1950),    (15):   [PMID:10358138]
4. Nakajima, H H, Cella, M M, Langen, H H, Friedlein, A A and Colonna, M M..  (1999)  Activating interactions in human NK cell recognition: the role of 2B4-CD48..  European journal of immunology,      [PMID:10359122]
5. Boles, K S KS and 7 more authors..  (1999)  Molecular characterization of a novel human natural killer cell receptor homologous to mouse 2B4..  Tissue antigens,      [PMID:10458320]
6. Kubin, M Z MZ and 13 more authors..  (1999)  Molecular cloning and biological characterization of NK cell activation-inducing ligand, a counterstructure for CD48..  European journal of immunology,      [PMID:10556801]
7. Parolini, S S and 12 more authors..  (2000)  X-linked lymphoproliferative disease. 2B4 molecules displaying inhibitory rather than activating function are responsible for the inability of natural killer cells to kill Epstein-Barr virus-infected cells..  The Journal of experimental medicine,    (7):   [PMID:10934222]
8. Kumaresan, P R PR and Mathew, P A PA..  (2000)  Structure of the human natural killer cell receptor 2B4 gene and identification of a novel alternative transcript..  Immunogenetics,      [PMID:11003394]
9. Watzl, C C, Stebbins, C C CC and Long, E O EO..  (2000)  NK cell inhibitory receptors prevent tyrosine phosphorylation of the activation receptor 2B4 (CD244)..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:11034353]
10. Speiser, D E DE and 10 more authors..  (2001)  The activatory receptor 2B4 is expressed in vivo by human CD8+ effector alpha beta T cells..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:11714776]
11. Aoukaty, Ala A and Tan, Rusung R..  (2002)  Association of the X-linked lymphoproliferative disease gene product SAP/SH2D1A with 2B4, a natural killer cell-activating molecule, is dependent on phosphoinositide 3-kinase..  The Journal of biological chemistry,    (12):   [PMID:11815622]
12. Sivori, Simona S and 7 more authors..  (2002)  Early expression of triggering receptors and regulatory role of 2B4 in human natural killer cell precursors undergoing in vitro differentiation..  Proceedings of the National Academy of Sciences of the United States of America,    (2):   [PMID:11917118]
13. Li, Chengjun C, Iosef, Cristiana C, Jia, Christina Y H CY, Han, Victor K M VK and Li, Shawn Shun-Cheng SS..  (2003)  Dual functional roles for the X-linked lymphoproliferative syndrome gene product SAP/SH2D1A in signaling through the signaling lymphocyte activation molecule (SLAM) family of immune receptors..  The Journal of biological chemistry,    (7):   [PMID:12458214]
14. Zhang, Zemin Z and Henzel, William J WJ..  (2004)  Signal peptide prediction based on analysis of experimentally verified cleavage sites..  Protein science : a publication of the Protein Society,      [PMID:15340161]
15. Eissmann, Philipp P and 5 more authors..  (2005)  Molecular basis for positive and negative signaling by the natural killer cell receptor 2B4 (CD244)..  Blood,    (15):   [PMID:15713798]
16. Mathew, Stephen O SO, Kumaresan, Pappanaicken R PR, Lee, Jae Kyung JK, Huynh, Van T VT and Mathew, Porunelloor A PA..  (2005)  Mutational analysis of the human 2B4 (CD244)/CD48 interaction: Lys68 and Glu70 in the V domain of 2B4 are critical for CD48 binding and functional activation of NK cells..  Journal of immunology (Baltimore, Md. : 1950),    (15):   [PMID:16002700]
17. Gregory, S G SG and 178 more authors..  (2006)  The DNA sequence and biological annotation of human chromosome 1..  Nature,    (18):   [PMID:16710414]
18. Mathew, Stephen O SO, Rao, Krithi K KK, Kim, Jong R JR, Bambard, Nowland D ND and Mathew, Porunelloor A PA..  (2009)  Functional role of human NK cell receptor 2B4 (CD244) isoforms..  European journal of immunology,      [PMID:19499526]
19. Betser-Cohen, Gili G, Mizrahi, Saar S, Elboim, Moran M, Alsheich-Bartok, Osnat O and Mandelboim, Ofer O..  (2010)  The association of MHC class I proteins with the 2B4 receptor inhibits self-killing of human NK cells..  Journal of immunology (Baltimore, Md. : 1950),    (15):   [PMID:20164429]
20. Margraf-Schönfeld, Stefanie S, Böhm, Carolin C and Watzl, Carsten C..  (2011)  Glycosylation affects ligand binding and function of the activating natural killer cell receptor 2B4 (CD244) protein..  The Journal of biological chemistry,    (8):   [PMID:21606496]
21. Wilson, Timothy J TJ and 5 more authors..  (2014)  Fine specificity and molecular competition in SLAM family receptor signalling..  PloS one,      [PMID:24642916]
22. Margraf, Stefanie S, Garner, Lee I LI, Wilson, Timothy J TJ and Brown, Marion H MH..  (2015)  A polymorphism in a phosphotyrosine signalling motif of CD229 (Ly9, SLAMF3) alters SH2 domain binding and T-cell activation..  Immunology,      [PMID:26221972]
23. Claus, Maren M, Wingert, Sabine S and Watzl, Carsten C..  (2016)  Modulation of natural killer cell functions by interactions between 2B4 and CD48 in cis and in trans..  Open biology,      [PMID:27249817]

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