| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp183653-10μg | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $139.90 | |
| rp183653-50μg | 50μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $399.90 | |
| rp183653-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $629.90 | |
| rp183653-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $3,699.90 |
Animal Free, ≥95% (SDS-PAGE), Active, HEK293, C-His, 1-502 aa
| Product Name | Recombinant Human Alkaline Phosphatase/ALPL Protein |
|---|---|
| Grade | ActiBioPure™, Animal Free, Bioactive, Carrier Free |
| Specifications & Purity | Animal Free, Carrier Free, Bioactive, ActiBioPure™, ≥95%(SDS-PAGE), Lot by Lot |
| Biochemical and Physiological Mechanisms | Alkaline phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name |
| Bioactivity | Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP) (M103814). The specific activity is >30,000 pmol/min/µg. |
| Endotoxin Concentration | <1.0 EU/μg |
| Amino Acids | 1-502 |
| Sequence | MISPFLVLAIGTCLTNSLVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDT |
| Predicted molecular weight | 54.2 kDa |
| SDS-PAGE | 59.1-72.1 kDa, under reducing conditions; 57.7-70.8 kDa, under non-reducing conditions. |
Recombinant Human Alkaline Phosphatase/ALPL Protein - Protein Bioactivity
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP) (M103814). The specific activity is >30,000 pmol/min/µg.
Recombinant Human Alkaline Phosphatase/ALPL Protein (rp183653) - SDS-PAGE
2 μg/lane of Recombinant Human Alkaline Phosphatase/ALPL Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 59.1-72.1 kDa under reducing conditions and 57.7-70.8 kDa under non-reducing conditions.
| Concentration | Lot by Lot |
|---|---|
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C for 1 year. Avoid freeze/thaw cycle. |
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