| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp142973-10μg | 10μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $69.90 | |
| rp142973-50μg | 50μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $329.90 | |
| rp142973-100μg | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $599.90 | |
| rp142973-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Animal Free, >95%(SDS-PAGE and HPLC), Active, E.coli, No tag, 25-182aa
| Product Name | Recombinant Human ARMET/ARP Protein, ＞95%(SDS-PAGE and HPLC), high purity |
|---|---|
| Synonyms | Arginine rich mutated in early stage tumors | Arginine rich protein | Arginine-rich protein | ARMET | ARMET protein | ARP | Manf | MANF_HUMAN | Mesencephalic astrocyte derived neurotrophic factor | Mesencephalic astrocyte-derived neurotrophic factor | MGC |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance |
| Product Description | Purity |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE&HPLC) |
| Purity | ＞95%(SDS-PAGE and HPLC) |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/ml, corresponding to a specific activity of > 50 IU/mg. |
| Endotoxin Concentration | <1.0 EU/μg |
| Expression System | E. coli |
| Species | Human |
| Amino Acids | 25-182aa |
| Sequence | LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL |
| Protein Tag | No tag |
| Accession # | P55145 |
| Predicted molecular weight | 18.2 kDa |
| SDS-PAGE | 18.2 kDa |
Recombinant Human ARMET/ARP Protein (rp142973)-Protein Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/mL, corresponding to a specific activity of > 50 IU/mg.
Recombinant Human ARMET/ARP Protein (rp142973)-SDS-PAGE
Recombinant Human ARMET/ARP Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. Showing a band at 18.2 kDa under reducing conditions and 16.8 kDa under non-reducing conditions.
| Form | Lyophilized |
|---|---|
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconst |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0600497 | Certificate of Analysis | Mar 26, 2024 | rp142973 |
| ZJ23F0600498 | Certificate of Analysis | Mar 26, 2024 | rp142973 |
| ZJ23F0600499 | Certificate of Analysis | Mar 26, 2024 | rp142973 |
| 1. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |
| 2. Shridhar, V V and 8 more authors.. (1996) A gene from human chromosomal band 3p21.1 encodes a highly conserved arginine-rich protein and is mutated in renal cell carcinomas.. Oncogene, (2): [PMID:8649854] |
| 3. Shridhar, R R and 10 more authors.. (1996) Mutations in the arginine-rich protein gene, in lung, breast, and prostate cancers, and in squamous cell carcinoma of the head and neck.. Cancer research, (15): [PMID:8971156] |
| 4. Shridhar, V V and 11 more authors.. (1997) Mutations in the arginine-rich protein gene (ARP) in pancreatic cancer.. Oncogene, (8): [PMID:9174057] |
| 5. Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801] |
| 6. Petrova, Penka P and 11 more authors.. (2003) MANF: a new mesencephalic, astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons.. Journal of molecular neuroscience : MN, [PMID:12794311] |
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| 8. Muzny, Donna M DM and 113 more authors.. (2006) The DNA sequence, annotation and analysis of human chromosome 3.. Nature, (27): [PMID:16641997] |
| 9. Apostolou, Andria A, Shen, Yuxian Y, Liang, Yan Y, Luo, Jun J and Fang, Shengyun S.. (2008) Armet, a UPR-upregulated protein, inhibits cell proliferation and ER stress-induced cell death.. Experimental cell research, (1): [PMID:18561914] |
| 10. Parkash, Vimal V and 7 more authors.. (2009) The structure of the conserved neurotrophic factors MANF and CDNF explains why they are bifunctional.. Protein engineering, design & selection : PEDS, [PMID:19258449] |
| 11. Glembotski, Christopher C CC and 5 more authors.. (2012) Mesencephalic astrocyte-derived neurotrophic factor protects the heart from ischemic damage and is selectively secreted upon sarco/endoplasmic reticulum calcium depletion.. The Journal of biological chemistry, (27): [PMID:22637475] |
| 12. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569] |
| 13. Bai, Meirong M and 11 more authors.. (2018) Conserved roles of C. elegans and human MANFs in sulfatide binding and cytoprotection.. Nature communications, (1): [PMID:29497057] |
