Bioactivity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/ml, corresponding to a specific activity of > 50 IU/mg.
Endotoxin Concentration: <1.0 EU/μg
Item Number
rp142973
Grouped product items
SKU
Size
Availability
Price
Qty
rp142973-10μg
10μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$69.90
rp142973-50μg
50μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$329.90
rp142973-100μg
100μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$599.90
rp142973-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
Recombinant Human ARMET/ARP Protein - Recombinant protein, >95%(SDS-PAGE and HPLC), high purity
Synonyms
Arginine rich mutated in early stage tumors | Arginine rich protein | Arginine-rich protein | ARMET | ARMET protein | ARP | Manf | MANF_HUMAN | Mesencephalic astrocyte derived neurotrophic factor | Mesencephalic astrocyte-derived neurotrophic factor | MGC
Grade
ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description
Purity >95% by SDS-PAGE and HPLC analyses.
Function Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death.
Post-translational May contain sialic acid residues.
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/ml, corresponding to a specific activity of > 50 IU/mg.
Recombinant Human ARMET/ARP Protein (rp142973)-Protein Bioactivity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 μg/mL, corresponding to a specific activity of > 50 IU/mg.
Recombinant Human ARMET/ARP Protein (rp142973)-SDS-PAGE Recombinant Human ARMET/ARP Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. Showing a band at 18.2 kDa under reducing conditions and 16.8 kDa under non-reducing conditions.
Product Specifications
Form
Lyophilized
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconst
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Find and download the COA for your product by matching the lot number on the packaging.
1.Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]
2.Shridhar, V V and 8 more authors.. (1996) A gene from human chromosomal band 3p21.1 encodes a highly conserved arginine-rich protein and is mutated in renal cell carcinomas.. Oncogene, (2):[PMID:8649854]
3.Shridhar, R R and 10 more authors.. (1996) Mutations in the arginine-rich protein gene, in lung, breast, and prostate cancers, and in squamous cell carcinoma of the head and neck.. Cancer research, (15):[PMID:8971156]
4.Shridhar, V V and 11 more authors.. (1997) Mutations in the arginine-rich protein gene (ARP) in pancreatic cancer.. Oncogene, (8):[PMID:9174057]
5.Gevaert, Kris K and 6 more authors.. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.. Nature biotechnology, [PMID:12665801]
6.Petrova, Penka P and 11 more authors.. (2003) MANF: a new mesencephalic, astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons.. Journal of molecular neuroscience : MN, [PMID:12794311]
7.Brill, Laurence M LM and 5 more authors.. (2004) Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.. Analytical chemistry, (15):[PMID:15144186]
8.Muzny, Donna M DM and 113 more authors.. (2006) The DNA sequence, annotation and analysis of human chromosome 3.. Nature, (27):[PMID:16641997]
9.Apostolou, Andria A, Shen, Yuxian Y, Liang, Yan Y, Luo, Jun J and Fang, Shengyun S.. (2008) Armet, a UPR-upregulated protein, inhibits cell proliferation and ER stress-induced cell death.. Experimental cell research, (1):[PMID:18561914]
10.Parkash, Vimal V and 7 more authors.. (2009) The structure of the conserved neurotrophic factors MANF and CDNF explains why they are bifunctional.. Protein engineering, design & selection : PEDS, [PMID:19258449]
11.Glembotski, Christopher C CC and 5 more authors.. (2012) Mesencephalic astrocyte-derived neurotrophic factor protects the heart from ischemic damage and is selectively secreted upon sarco/endoplasmic reticulum calcium depletion.. The Journal of biological chemistry, (27):[PMID:22637475]
12.Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16):[PMID:24275569]
13.Bai, Meirong M and 11 more authors.. (2018) Conserved roles of C. elegans and human MANFs in sulfatide binding and cytoprotection.. Nature communications, (1):[PMID:29497057]
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