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Recombinant Human β2-microglobulin Protein

  • Azide Free
  • Carrier Free
  • ≥95%(SDS-PAGE)
Features and benefits
  • Expression System: E. coli
  • Protein Tag: N-His
  • Endotoxin Concentration: <1 EU/μg
Item Number
rp169553
Grouped product items
SKUSizeAvailabilityPrice Qty
rp169553-10μg (Trial Size)
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10μg
In stock
$59.90
rp169553-50μg
50μg
In stock
$139.90
rp169553-100μg
100μg
In stock
$199.90
rp169553-1mg
1mg
In stock
$1,399.90

Carrier free, >95% SDS-PAGE, E.coli, N-His Tag, 21-119 aa

Basic Description

Product NameRecombinant Human β2-microglobulin Protein
SynonymsBeta-2-Microglobulin; Beta Chain Of MHC Class I Molecules; IMD43; B2MB2MG_HUMAN; Beta 2 microglobulin; Beta 2 microglobulin precursor; Beta chain of mhc class 1 proteins; Beta chain of MHC class I molecules; Beta-2-microglobulin form pI 5.3; CDABP0092; Hd
GradeAzide Free, Carrier Free
Product Description

Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.

Description:

B2M (Beta-2-Microglobulin) is a Protein Coding gene. Diseases associated with B2M include Immunodeficiency 43 and Amyloidosis, Familial Visceral. Among its related pathways are SARS-CoV-2 Infection and Infectious disease. Gene Ontology (GO) annotations related to this gene include identical protein binding. An important paralog of this gene is HLA-DPA1.

Specifications & PurityCarrier Free, Azide Free, ≥95%(SDS-PAGE)
Endotoxin Concentration<1 EU/μg
Expression SystemE. coli
Amino Acids21-119 aa
SequenceMGSSHHHHHHSSGLVPRGSHMIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
Protein TagN-His
Predicted molecular weight14.0 kDa
SDS-PAGE14.0 kDa, reducing conditions; 14.0 kDa, non-reducing conditions

Images

Recombinant Human Beta-2 Microglobulin Protein (rp169553) - SDS-PAGE
3 μg/lane of Recombinant Human Beta-2 Microglobulin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. Showing a band at 14 kDa.

Product Specifications

FormLyophilized
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C stable up to 1 year. Avoid freeze / thaw cycle.

Certificates

Certificate of Analysis(COA)

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5 results found

Lot NumberCertificate TypeDateItem
ZJ23F1201878Certificate of AnalysisDec 13, 2023 rp169553
ZJ23F1201877Certificate of AnalysisDec 13, 2023 rp169553
ZJ23F1201876Certificate of AnalysisDec 13, 2023 rp169553
ZJ23F1201875Certificate of AnalysisDec 13, 2023 rp169553
ZJ23R1100162Certificate of AnalysisNov 24, 2023 rp169553

Related Documents

References

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12. Haag-Weber, M M, Mai, B B and Hörl, W H WH..  (1994)  Isolation of a granulocyte inhibitory protein from uraemic patients with homology of beta 2-microglobulin..  Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,      [PMID:8084451]
13. Smith, K J KJ and 6 more authors..  (1996)  Bound water structure and polymorphic amino acids act together to allow the binding of different peptides to MHC class I HLA-B53..  Immunity,      [PMID:8624812]
14. Braud, V M VM, Allan, D S DS, Wilson, D D and McMichael, A J AJ..  (1998)  TAP- and tapasin-dependent HLA-E surface expression correlates with the binding of an MHC class I leader peptide..  Current biology : CB,    (1):   [PMID:9427624]
15. Wainwright, S D SD, Biro, P A PA and Holmes, C H CH..  (2000)  HLA-F is a predominantly empty, intracellular, TAP-associated MHC class Ib protein with a restricted expression pattern..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:10605026]
16. Rosa, F F and 5 more authors..  (1983)  The beta2-microglobulin mRNA in human Daudi cells has a mutated initiation codon but is still inducible by interferon..  The EMBO journal,      [PMID:11894933]
17. Trinh, Chi H CH, Smith, David P DP, Kalverda, Arnout P AP, Phillips, Simon E V SE and Radford, Sheena E SE..  (2002)  Crystal structure of monomeric human beta-2-microglobulin reveals clues to its amyloidogenic properties..  Proceedings of the National Academy of Sciences of the United States of America,    (23):   [PMID:12119416]
18. Stewart-Jones, Guillaume B E GB, McMichael, Andrew J AJ, Bell, John I JI, Stuart, David I DI and Jones, E Yvonne EY..  (2003)  A structural basis for immunodominant human T cell receptor recognition..  Nature immunology,      [PMID:12796775]
19. He, Xian-Hui XH, Xu, Li-Hui LH, Liu, Yi Y and Zeng, Yao-Ying YY..  (2004)  [Cloning of human beta-microglobulin gene and its high expression in Escherichia coli]..  Sheng wu gong cheng xue bao = Chinese journal of biotechnology,      [PMID:16108498]
20. Eakin, Catherine M CM, Berman, Andrea J AJ and Miranker, Andrew D AD..  (2006)  A native to amyloidogenic transition regulated by a backbone trigger..  Nature structural & molecular biology,      [PMID:16491088]
21. Deng, Nan-Jie NJ, Yan, Lisa L, Singh, Deepak D and Cieplak, Piotr P..  (2006)  Molecular basis for the Cu2+ binding-induced destabilization of beta2-microglobulin revealed by molecular dynamics simulation..  Biophysical journal,    (1):   [PMID:16513784]
22. Wani, Manzoor A MA and 8 more authors..  (2006)  Familial hypercatabolic hypoproteinemia caused by deficiency of the neonatal Fc receptor, FcRn, due to a mutant beta2-microglobulin gene..  Proceedings of the National Academy of Sciences of the United States of America,    (28):   [PMID:16549777]
23. Kihara, Miho M and 7 more authors..  (2006)  Conformation of amyloid fibrils of beta2-microglobulin probed by tryptophan mutagenesis..  The Journal of biological chemistry,    (13):   [PMID:16901902]
24. Shiroishi, Mitsunori M and 8 more authors..  (2006)  Structural basis for recognition of the nonclassical MHC molecule HLA-G by the leukocyte Ig-like receptor B2 (LILRB2/LIR2/ILT4/CD85d)..  Proceedings of the National Academy of Sciences of the United States of America,    (31):   [PMID:17056715]
25. Iwata, Kentaro K, Matsuura, Takanori T, Sakurai, Kazumasa K, Nakagawa, Atsushi A and Goto, Yuji Y..  (2007)  High-resolution crystal structure of beta2-microglobulin formed at pH 7.0..  Journal of biochemistry,      [PMID:17646174]
26. Esposito, Gennaro G and 16 more authors..  (2008)  The controlling roles of Trp60 and Trp95 in beta2-microglobulin function, folding and amyloid aggregation properties..  Journal of molecular biology,    (9):   [PMID:18395224]
27. Ricagno, Stefano S and 7 more authors..  (2008)  DE loop mutations affect beta2-microglobulin stability and amyloid aggregation..  Biochemical and biophysical research communications,    (5):   [PMID:18835253]
28. Ricagno, Stefano S, Raimondi, Sara S, Giorgetti, Sofia S, Bellotti, Vittorio V and Bolognesi, Martino M..  (2009)  Human beta-2 microglobulin W60V mutant structure: Implications for stability and amyloid aggregation..  Biochemical and biophysical research communications,    (13):   [PMID:19284997]
29. Giorgetti, Sofia S and 17 more authors..  (2011)  Effect of tetracyclines on the dynamics of formation and destructuration of beta2-microglobulin amyloid fibrils..  The Journal of biological chemistry,    (21):   [PMID:21068391]
30. Valleix, Sophie S and 25 more authors..  (2012)  Hereditary systemic amyloidosis due to Asp76Asn variant β2-microglobulin..  The New England journal of medicine,    (14):   [PMID:22693999]
31. Sreejit, Gopalkrishna G and 6 more authors..  (2014)  The ESAT-6 protein of Mycobacterium tuberculosis interacts with beta-2-microglobulin (β2M) affecting antigen presentation function of macrophage..  PLoS pathogens,      [PMID:25356553]

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