Click Here for 5% Off Your First Aladdin Purchase!
Call +1 (833) 552-7181 or Contact us Earn $25 in coupons on your first order with Aladdin!
Toggle Nav
My Cart
Search
Advanced Search
Menu
Sign In
྾
main product photo

Recombinant Human B7-1/CD80 Protein, ≥95% (SDS-PAGE), high purity

  • ActiBioPure™
  • Animal Free
  • Azide Free
  • Bioactive
  • Carrier Free
  • ≥95%(SDS-PAGE)
Features and benefits
  • Expression System: HEK293
  • Accession #: P33681
  • Protein Tag: C-His
  • Bioactivity: Immobilized Recombinant Human B7-1/CD80 Protein (rp144120) at 2.0 μg/mL can bind Recombinant human CTLA-4 with a linear range of 7.0 - 62.0 ng/mL. The EC50 for this effect is 23.47 ng/mL.
  • Endotoxin Concentration: <0.1 EU/μg
Item Number
rp144120
Grouped product items
SKUSizeAvailabilityPrice Qty
rp144120-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$49.90
rp144120-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$139.90
rp144120-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,599.90
Add to Wish List Compare
Bulk Order  DATASHEET

Animal Free, ≥95% (SDS-PAGE), Active, 293F, C-His tag, 35-242 aa

View related series
Accession#:P33681 B7-1 CD80 Gene ID:941

Basic Description

Product NameRecombinant Human B7-1/CD80 Protein, ≥95% (SDS-PAGE), high purity
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description

Purity
≥95% (SDS-PAGE)
Endotoxin level
<0.1 EU/μg
Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
Purity≥95% (SDS-PAGE)
BioactivityImmobilized Recombinant Human B7-1/CD80 Protein (rp144120) at 2.0 μg/mL can bind Recombinant human CTLA-4 with a linear range of 7.0 - 62.0 ng/mL. The EC50 for this effect is 23.47 ng/mL.
Endotoxin Concentration<0.1 EU/μg
Expression SystemHEK293
SpeciesHuman
Amino Acids35-242 aa
SequenceVIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDNHHHHHH
Protein TagC-His
Accession #P33681
Predicted molecular weight25 kDa
SDS-PAGE40 - 55 kDa, under reducing conditions; 40 - 55 kDa, under non-reducing conditions.

Product Specifications

FormLyophilized
ReconstitutionReconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Related Documents

 SDS

 Specification Sheet

References

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al..  (2004)  The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)..  Genome Res,  14  (10B): (2121-7).  [PMID:15489334]
2. Vicente Rabaneda EF, Herrero-Beaumont G, Castañeda S.  (2013)  Update on the use of abatacept for the treatment of rheumatoid arthritis..  Expert Rev Clin Immunol,  9  (7): (599-621).  [PMID:23899231]
3. Linsley PS, Brady W, Urnes M, Grosmaire LS, Damle NK, Ledbetter JA.  (1991)  CTLA-4 is a second receptor for the B cell activation antigen B7..  J Exp Med,  174  (3): (561-9).  [PMID:1714933]
4. Selvakumar, A A and 5 more authors..  (1992)  Genomic organization and chromosomal location of the human gene encoding the B-lymphocyte activation antigen B7..  Immunogenetics,      [PMID:1377173]
5. Freeman, G J GJ and 8 more authors..  (1991)  Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7..  The Journal of experimental medicine,    (1):   [PMID:1714935]
6. Freeman, G J GJ and 5 more authors..  (1989)  B7, a new member of the Ig superfamily with unique expression on activated and neoplastic B cells..  Journal of immunology (Baltimore, Md. : 1950),    (15):   [PMID:2794510]
7. Lanier, L L LL and 7 more authors..  (1995)  CD80 (B7) and CD86 (B70) provide similar costimulatory signals for T cell proliferation, cytokine production, and generation of CTL..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:7527824]
8. Vandenborre, K K and 5 more authors..  (1999)  Interaction of CTLA-4 (CD152) with CD80 or CD86 inhibits human T-cell activation..  Immunology,      [PMID:10583602]
9. Ikemizu, S S and 7 more authors..  (2000)  Structure and dimerization of a soluble form of B7-1..  Immunity,      [PMID:10661405]
10. Gottlieb, Alice B AB and 8 more authors..  (2004)  Evaluation of safety and clinical activity of multiple doses of the anti-CD80 monoclonal antibody, galiximab, in patients with moderate to severe plaque psoriasis..  Clinical immunology (Orlando, Fla.),      [PMID:15093549]
11. Czuczman, Myron S MS and 13 more authors..  (2005)  Phase I/II study of galiximab, an anti-CD80 antibody, for relapsed or refractory follicular lymphoma..  Journal of clinical oncology : official journal of the American Society of Clinical Oncology,    (1):   [PMID:15994148]
12. and Kremer, Joel M JM..  (2005)  Selective costimulation modulators: a novel approach for the treatment of rheumatoid arthritis..  Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases,      [PMID:16357751]
13. Hervey, Pauline S PS and Keam, Susan J SJ..  (2006)  Abatacept..  BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy,      [PMID:16573350]
14. Muzny, Donna M DM and 113 more authors..  (2006)  The DNA sequence, annotation and analysis of human chromosome 3..  Nature,    (27):   [PMID:16641997]
15. Weyand, Cornelia M CM and Goronzy, Jörg J JJ..  (2006)  T-cell-targeted therapies in rheumatoid arthritis..  Nature clinical practice. Rheumatology,      [PMID:16932686]
16. and Scheinfeld, Noah N..  (2006)  Abatacept: A review of a new biologic agent for refractory rheumatoid arthritis for dermatologists..  The Journal of dermatological treatment,      [PMID:16971318]
17. Tedesco Silva, Helio H, Pinheiro Machado, Paula P, Rosso Felipe, Claudia C and Medina Pestana, Jose Osmar JO..  (2006)  Immunotherapy for De Novo renal transplantation: what's in the pipeline?.  Drugs,      [PMID:16978033]
18. Vincenti, Flavio F and Luggen, Michael M..  (2007)  T cell costimulation: a rational target in the therapeutic armamentarium for autoimmune diseases and transplantation..  Annual review of medicine,      [PMID:17020493]
19. Nogid, Anna A and Pham, David Q DQ..  (2006)  Role of abatacept in the management of rheumatoid arthritis..  Clinical therapeutics,      [PMID:17212998]
20. Leonard, J P JP and 14 more authors..  (2007)  A phase I/II study of galiximab (an anti-CD80 monoclonal antibody) in combination with rituximab for relapsed or refractory, follicular lymphoma..  Annals of oncology : official journal of the European Society for Medical Oncology,      [PMID:17470451]
21. Kakoulidou, M M, Giscombe, R R, Zhao, X X, Lefvert, A K AK and Wang, X X..  (2007)  Human Soluble CD80 is generated by alternative splicing, and recombinant soluble CD80 binds to CD28 and CD152 influencing T-cell activation..  Scandinavian journal of immunology,      [PMID:17953528]
22. Reynolds, Jennifer J, Shojania, Kam K and Marra, Carlo A CA..  (2007)  Abatacept: a novel treatment for moderate-to-severe rheumatoid arthritis..  Pharmacotherapy,      [PMID:18041889]
23. Maxwell, Lara J LJ and Singh, Jasvinder A JA..  (2010)  Abatacept for rheumatoid arthritis: a Cochrane systematic review..  The Journal of rheumatology,      [PMID:20080922]
24. Kleinpeter, Patricia and 20 more authors..  (2019)  By Binding CD80 and CD86, the Vaccinia Virus M2 Protein Blocks Their Interactions with both CD28 and CTLA4 and Potentiates CD80 Binding to PD-L1..  Journal of virology,    (1):   [PMID:30918073]

Solution Calculators

Privacy Policy Website Terms of Use Return policy Terms and Conditions of Sale Cookie Policy Sales Policy
Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.
Copyright © 2023-present Aladdin Scientific Corp . All rights reserved.