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Recombinant human BACE1 protein, ≥90% (SDS-PAGE), high purity(Active)

  • ActiBioPure™
  • Animal Free
  • Azide Free
  • Bioactive
  • Carrier Free
  • ≥90%(SDS-PAGE)
Features and benefits
  • Expression System: HEK293
  • Accession #: P56817
  • Protein Tag: C-His
  • Bioactivity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is >5.5 pmol/min/μg.
  • Endotoxin Concentration: <0.1 EU/μg
Item Number
rp143119
Grouped product items
SKUSizeAvailabilityPrice Qty
rp143119-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
rp143119-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$139.90
rp143119-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$249.90
rp143119-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,959.90

Animal Free, ≥90% (SDS-PAGE), Active, 293F, C-His tag, 22-460 aa

Basic Description

Product NameRecombinant human BACE1 protein, ≥90% (SDS-PAGE), high purity
SynonymsAPP beta-secretase; ASP2; Aspartyl protease 2; BACE1; BACE-1; BACEAsp 2; beta-secretase 1 precursor variant 1; beta-secretase 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description

Purity
≥90% (SDS-PAGE)
Endotoxin level
<0.1 EU/μg
Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
Post-translational
Glycosylated.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥90%(SDS-PAGE)
Purity≥90% (SDS-PAGE)
BioactivityMeasured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is >5.5 pmol/min/μg.
Endotoxin Concentration<0.1 EU/μg
Expression SystemHEK293
SpeciesHuman
Amino Acids22-460 aa
SequenceTQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKM
Protein TagC-His
Accession #P56817
Predicted molecular weight49 kDa
SDS-PAGE60-70 kDa

Images

Recombinant Human BACE1 Protein (rp143119) - SDS-PAGE
3 μg/lane of Recombinant Human BACE1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 60.0 - 70.0 kDa under reducing conditions and 55.0 - 60.0 kDa under non-reducing conditions.

Product Specifications

FormLyophilized
ReconstitutionReconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505374Certificate of AnalysisMay 15, 2024 rp143119
ZJ24F0505373Certificate of AnalysisMay 15, 2024 rp143119
ZJ24F0505372Certificate of AnalysisMay 15, 2024 rp143119

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