Recombinant Human beta 2 Microglobulin Protein - ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE), high purity

Item Number
H293861
Grouped product items
SKUSizeAvailabilityPrice Qty
H293861-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
H293861-50μg
50μg
In stock
$139.90
H293861-100μg
100μg
In stock
$199.90
H293861-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,429.90

Animal Free, ≥95% (SDS-PAGE), Active, HEK293, C-His tag, 21-119 aa

Basic Description

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
Stability And StorageStore at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description

Purity
≥95% SDS-PAGE.
Endotoxin level
<0.1 EU/µg
Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

Images

Recombinant Human beta 2 Microglobulin Protein (H293861) - Protein Bioactivity
Immobilized Recombinant Human beta 2 Microglobulin Protein (H293861) at 2.0 μg/mL can bind Recombinant Human CD8 alpha with a linear range of 30 - 350 ng/mL. The ED₅₀ for this effect is 212.5 ng/mL.

Recombinant Human beta 2 Microglobulin Protein (H293861) - SDS-PAGE
3 μg/lane of Recombinant Human beta 2 Microglobulin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 12.5 kDa.

Certificates

Certificate of Analysis(COA)

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4 results found

Lot NumberCertificate TypeDateItem
ZJ24F0404881Certificate of AnalysisApr 26, 2024 H293861
ZJ24F0404715Certificate of AnalysisApr 26, 2024 H293861
ZJ24F0404714Certificate of AnalysisApr 26, 2024 H293861
ZJ24F0404713Certificate of AnalysisApr 26, 2024 H293861

Related Documents

References

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al..  (2004)  The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)..  Genome Res,  14  (10B): (2121-7).  [PMID:15489334] [10.1021/op500134e]
2. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al..  (2004)  Complete sequencing and characterization of 21,243 full-length human cDNAs..  Nat Genet,  36  (1): (40-5).  [PMID:14702039] [10.1021/op500134e]
3. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]
4. Argilés, A A, Derancourt, J J, Jauregui-Adell, J J, Mion, C C and Demaille, J G JG..  (1992)  Biochemical characterization of serum and urinary beta 2 microglobulin in end-stage renal disease patients..  Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,      [PMID:1336137]
5. Okon, M M, Bray, P P and Vucelić, D D..  (1992)  1H NMR assignments and secondary structure of human beta 2-microglobulin in solution..  Biochemistry,    (22):   [PMID:1390678]
6. Saper, M A MA, Bjorkman, P J PJ and Wiley, D C DC..  (1991)  Refined structure of the human histocompatibility antigen HLA-A2 at 2.6 A resolution..  Journal of molecular biology,    (20):   [PMID:2038058]
7. Bjorkman, P J PJ and 5 more authors..  (1987)  Structure of the human class I histocompatibility antigen, HLA-A2..  Nature,      [PMID:3309677]
8. Güssow, D D and 6 more authors..  (1987)  The human beta 2-microglobulin gene. Primary structure and definition of the transcriptional unit..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:3312414]
9. Gorevic, P D PD and 8 more authors..  (1986)  Polymerization of intact beta 2-microglobulin in tissue causes amyloidosis in patients on chronic hemodialysis..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:3532124]
10. Cunningham, B A BA, Wang, J L JL, Berggård, I I and Peterson, P A PA..  (1973)  The complete amino acid sequence of beta 2-microglobulin..  Biochemistry,    (20):   [PMID:4586824]
11. Suggs, S V SV, Wallace, R B RB, Hirose, T T, Kawashima, E H EH and Itakura, K K..  (1981)  Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:6171820]
12. Momoi, T T and 5 more authors..  (1995)  Amino acid sequence of a modified beta 2-microglobulin in renal failure patient urine and long-term dialysis patient blood..  Clinica chimica acta; international journal of clinical chemistry,    (15):   [PMID:7554280]
13. Collins, E J EJ, Garboczi, D N DN, Karpusas, M N MN and Wiley, D C DC..  (1995)  The three-dimensional structure of a class I major histocompatibility complex molecule missing the alpha 3 domain of the heavy chain..  Proceedings of the National Academy of Sciences of the United States of America,    (14):   [PMID:7862664]
14. Miyata, T T and 7 more authors..  (1994)  Glycation of human beta 2-microglobulin in patients with hemodialysis-associated amyloidosis: identification of the glycated sites..  Biochemistry,    (11):   [PMID:7918443]
15. Haag-Weber, M M, Mai, B B and Hörl, W H WH..  (1994)  Isolation of a granulocyte inhibitory protein from uraemic patients with homology of beta 2-microglobulin..  Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,      [PMID:8084451]
16. Smith, K J KJ and 6 more authors..  (1996)  Bound water structure and polymorphic amino acids act together to allow the binding of different peptides to MHC class I HLA-B53..  Immunity,      [PMID:8624812]
17. Braud, V M VM, Allan, D S DS, Wilson, D D and McMichael, A J AJ..  (1998)  TAP- and tapasin-dependent HLA-E surface expression correlates with the binding of an MHC class I leader peptide..  Current biology : CB,    (1):   [PMID:9427624]
18. Wainwright, S D SD, Biro, P A PA and Holmes, C H CH..  (2000)  HLA-F is a predominantly empty, intracellular, TAP-associated MHC class Ib protein with a restricted expression pattern..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:10605026]
19. Rosa, F F and 5 more authors..  (1983)  The beta2-microglobulin mRNA in human Daudi cells has a mutated initiation codon but is still inducible by interferon..  The EMBO journal,      [PMID:11894933]
20. Trinh, Chi H CH, Smith, David P DP, Kalverda, Arnout P AP, Phillips, Simon E V SE and Radford, Sheena E SE..  (2002)  Crystal structure of monomeric human beta-2-microglobulin reveals clues to its amyloidogenic properties..  Proceedings of the National Academy of Sciences of the United States of America,    (23):   [PMID:12119416]
21. Stewart-Jones, Guillaume B E GB, McMichael, Andrew J AJ, Bell, John I JI, Stuart, David I DI and Jones, E Yvonne EY..  (2003)  A structural basis for immunodominant human T cell receptor recognition..  Nature immunology,      [PMID:12796775]
22. He, Xian-Hui XH, Xu, Li-Hui LH, Liu, Yi Y and Zeng, Yao-Ying YY..  (2004)  [Cloning of human beta-microglobulin gene and its high expression in Escherichia coli]..  Sheng wu gong cheng xue bao = Chinese journal of biotechnology,      [PMID:16108498]
23. Eakin, Catherine M CM, Berman, Andrea J AJ and Miranker, Andrew D AD..  (2006)  A native to amyloidogenic transition regulated by a backbone trigger..  Nature structural & molecular biology,      [PMID:16491088]
24. Deng, Nan-Jie NJ, Yan, Lisa L, Singh, Deepak D and Cieplak, Piotr P..  (2006)  Molecular basis for the Cu2+ binding-induced destabilization of beta2-microglobulin revealed by molecular dynamics simulation..  Biophysical journal,    (1):   [PMID:16513784]
25. Wani, Manzoor A MA and 8 more authors..  (2006)  Familial hypercatabolic hypoproteinemia caused by deficiency of the neonatal Fc receptor, FcRn, due to a mutant beta2-microglobulin gene..  Proceedings of the National Academy of Sciences of the United States of America,    (28):   [PMID:16549777]
26. Kihara, Miho M and 7 more authors..  (2006)  Conformation of amyloid fibrils of beta2-microglobulin probed by tryptophan mutagenesis..  The Journal of biological chemistry,    (13):   [PMID:16901902]
27. Shiroishi, Mitsunori M and 8 more authors..  (2006)  Structural basis for recognition of the nonclassical MHC molecule HLA-G by the leukocyte Ig-like receptor B2 (LILRB2/LIR2/ILT4/CD85d)..  Proceedings of the National Academy of Sciences of the United States of America,    (31):   [PMID:17056715]
28. Iwata, Kentaro K, Matsuura, Takanori T, Sakurai, Kazumasa K, Nakagawa, Atsushi A and Goto, Yuji Y..  (2007)  High-resolution crystal structure of beta2-microglobulin formed at pH 7.0..  Journal of biochemistry,      [PMID:17646174]
29. Esposito, Gennaro G and 16 more authors..  (2008)  The controlling roles of Trp60 and Trp95 in beta2-microglobulin function, folding and amyloid aggregation properties..  Journal of molecular biology,    (9):   [PMID:18395224]
30. Ricagno, Stefano S and 7 more authors..  (2008)  DE loop mutations affect beta2-microglobulin stability and amyloid aggregation..  Biochemical and biophysical research communications,    (5):   [PMID:18835253]
31. Ricagno, Stefano S, Raimondi, Sara S, Giorgetti, Sofia S, Bellotti, Vittorio V and Bolognesi, Martino M..  (2009)  Human beta-2 microglobulin W60V mutant structure: Implications for stability and amyloid aggregation..  Biochemical and biophysical research communications,    (13):   [PMID:19284997]
32. Giorgetti, Sofia S and 17 more authors..  (2011)  Effect of tetracyclines on the dynamics of formation and destructuration of beta2-microglobulin amyloid fibrils..  The Journal of biological chemistry,    (21):   [PMID:21068391]
33. Valleix, Sophie S and 25 more authors..  (2012)  Hereditary systemic amyloidosis due to Asp76Asn variant β2-microglobulin..  The New England journal of medicine,    (14):   [PMID:22693999]
34. Bian, Yangyang Y and 9 more authors..  (2014)  An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome..  Journal of proteomics,    (16):   [PMID:24275569]
35. Sreejit, Gopalkrishna G and 6 more authors..  (2014)  The ESAT-6 protein of Mycobacterium tuberculosis interacts with beta-2-microglobulin (β2M) affecting antigen presentation function of macrophage..  PLoS pathogens,      [PMID:25356553]

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