| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp143234-10μg | 10μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $69.90 | |
| rp143234-50μg | 50μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $329.90 | |
| rp143234-100μg | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $529.90 | |
| rp143234-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Animal Free, >98%(SDS-PAGE, HPLC), Active, E.coli, No tag, 22-68 aa
| Product Name | Recombinant Human beta-Defensin 1 Protein, ＞98%(SDS-PAGE, HPLC), high purityused for Active, SDS-PAGE various applications |
|---|---|
| Synonyms | BD 1 | BD-1 | BD1 | beta 1 | Beta defensin 1 | Beta-defensin 1 | DEFB 1 | DEFB1 | DEFB1_HUMAN | DEFB101 | Defensin | Defensin beta 1 | Defensin beta 1 preproprotein | HBD 1 | hBD-1 | HBD1 | MGC51822 | DEFB-1 |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance |
| Product Description | Purity > 98 % by SDS-PAGE and HPLC analyses. Endotoxin level is less than 1EU/µg. Function Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human β-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because β-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. The β-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensin 1 may play a role in the pathogenesis of severe sepsis. Variation in human Beta Defensin-1 contributes to asthma diagnosis, with apparent gender-specific effects. Variation in human β-defensins 1 contributes to asthma diagnosis, with apparent gender-specific effects. Human BD1 is down-regulated in human prostatic and renal carcinomas. |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥98%(SDS-PAGE&HPLC) |
| Purity | ＞98%(SDS-PAGE, HPLC) |
| Bioactivity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100.0-1000.0 ng/ml. |
| Endotoxin Concentration | <1.0 EU/μg |
| Expression System | E.coli |
| Species | Human |
| Amino Acids | 22-68 aa |
| Sequence | GNFLTGLGHR SDHYNCVSSG GQCLYSACPI FTKIQGTCYR GKAKCCK |
| Protein Tag | No tag |
| Protein Length | Full length protein |
| Accession # | P60022 |
| Source | Recombinant |
| Predicted molecular weight | 5.1 kDa |
| SDS-PAGE | 8.0 kDa, under reducing conditions; 15.8 kDa, under non-reducing conditions. |
Recombinant Human beta-Defensin 1 Protein (rp143234)-Protein Bioactivity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100.0-1000.0 ng/mL.
Recombinant Human beta-Defensin 1 Protein (rp143234)-SDS-PAGE
Recombinant Human beta-Defensin 1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 8.0 kDa under reducing conditions and 15.8 kDa under non-reducing conditions.
| Application | Active, SDS-PAGE |
|---|---|
| Form | Lyophilized |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ℃ as supplied; 1 month, 2 to 8 ℃ under sterile conditions after reconstitution; 3 months, -20 to -70 ℃ under sterile conditions after reconstitu |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0600425 | Certificate of Analysis | Jun 21, 2023 | rp143234 |
| ZJ23F0600426 | Certificate of Analysis | Jun 21, 2023 | rp143234 |
| ZJ23F0600427 | Certificate of Analysis | Jun 21, 2023 | rp143234 |
