| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp143202-10μg | 10μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $89.90 | |
| rp143202-50μg | 50μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $309.90 | |
| rp143202-100μg | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $529.90 | |
| rp143202-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Animal Free, >98%(SDS-PAGE, HPLC), Active, E.coli, No tag, 23-67 aa
| Product Name | Recombinant Human Beta-Defensin 3 Protein, ＞98%(SDS-PAGE, HPLC), high purity |
|---|---|
| Synonyms | BD14 | BD3 | BD-3 | beta-defensin 103 | betaDefensin 3 | beta-Defensin 3 | DEFB103 | DEFB103A | DEFB103B | DEFB3 | DEFB-3 | DEFB3DEFB103A | Defensin, beta 103 | defensin, beta 103B | Defensin-like protein | HBD-3 | HBD3 | HBP3 | HBP-3 | BD-3 | Beta-defens |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free |
| Product Description | Purity > 98% by SDS-PAGE and HPLC analyses. Function Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistent E.faecium. No significant hemolytic activity was observe. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human β-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because β-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. |
| Specifications & Purity | Animal Free, Carrier Free, Bioactive, ActiBioPure™, Azide Free, ≥98%(SDS-PAGE&HPLC), 0.5M in TMF |
| Purity | ＞98%(SDS-PAGE, HPLC) |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 30 μg/ml. |
| Endotoxin Concentration | <1.0 EU/μg |
| Expression System | E.coli |
| Species | Human |
| Amino Acids | 23-67 aa |
| Sequence | GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK |
| Protein Tag | No tag |
| Accession # | P81534 |
| Predicted molecular weight | 5.2 kDa |
Recombinant Human Beta-Defensin 3 Protein (rp143202)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by anti-microbial activity against E.coli. is less than 30 μg/mL, corresponding to a specific activity of > 33.3 IU/mg.
Recombinant Human Beta-Defensin 3 Protein (rp143202)-SDS-PAGE
Recombinant Human Beta-Defensin 3 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a single band at 5.2 kDa.
| Form | Lyophilized |
|---|---|
| Concentration | 0.5M in TMF |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ℃ as supplied; 1 month, 2 to 8 ℃ under sterile conditions after reconstitution; 3 months, -20 to -70 ℃ under sterile conditions after reconstitu |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0600350 | Certificate of Analysis | Mar 26, 2024 | rp143202 |
| ZJ23F0600351 | Certificate of Analysis | Mar 26, 2024 | rp143202 |
| ZJ23F0600352 | Certificate of Analysis | Mar 26, 2024 | rp143202 |
| 1. Harder, J J, Bartels, J J, Christophers, E E and Schroder, J M JM.. (2001) Isolation and characterization of human beta -defensin-3, a novel human inducible peptide antibiotic.. The Journal of biological chemistry, (23): [PMID:11085990] |
| 2. Jia, H P HP and 10 more authors.. (2001) Discovery of new human beta-defensins using a genomics-based approach.. Gene, (24): [PMID:11223260] |
| 3. Schibli, David J DJ and 7 more authors.. (2002) The solution structures of the human beta-defensins lead to a better understanding of the potent bactericidal activity of HBD3 against Staphylococcus aureus.. The Journal of biological chemistry, (8): [PMID:11741980] |
