Bioactivity: Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit BMP-2-induced activity in murine MC3T3‑E1 cells.
Endotoxin Concentration: <1.0 EU/μg
Item Number
rp143300
Grouped product items
SKU
Size
Availability
Price
Qty
rp143300-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$969.90
rp143300-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90
rp143300-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$4,299.90
rp143300-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
Recombinant Human BMP3 Protein, >95% (SDS-PAGE&HPLC), high purity
Synonyms
BMP3 | BMP-3 | BMP3A | BMP-3A | Bone morphogenetic protein 3 (osteogenic) | Bone morphogenetic protein 3 | Bone morphogenetic protein 3A | Bone morphogenetic protein-3 | Osteogenin | BMP-3 | BMP-3A | Bone morphogenetic protein 3A | Osteogenin
Grade
ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description
Purity >95% (SDS-PAGE&HPLC) Endotoxin level <1.0 EU/μg Function Bone morphogenetic protein 3, also known as osteogenic, is a protein in humans that is encoded by the BMP3 gene. The protein encoded by this gene is a member of the transforming growth factor-beta superfamily. It, like other bone morphogenetic proteins (BMP's), is known for its ability to induce bone and cartilage development. It is a disulfide-linked homodimer. It negatively regulates bone density. BMP3 is an antagonist to other BMP's in the differentiation of osteogenic progenitors. It is highly expressed in fractured tissues. Negatively regulates bone density. Antagonizes the ability of certain osteogenic BMPs to induce osteoprogenitor differentitation and ossification.
Recombinant Human BMP3 Protein (rp143300) - SDS-PAGE Recombinant Human BMP3 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 24.8 kDa.
Product Specifications
Form
Lyophilized
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Fur
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. Upon delivery aliquot. Avoid freeze/thaw cycle.
Certificates
Certificate of Analysis(COA)
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1.Wozney, J M JM and 7 more authors.. (1988) Novel regulators of bone formation: molecular clones and activities.. Science (New York, N.Y.), (16):[PMID:3201241]
2.Daluiski, A A and 8 more authors.. (2001) Bone morphogenetic protein-3 is a negative regulator of bone density.. Nature genetics, [PMID:11138004]
3.Kloen, P P and 6 more authors.. (2003) BMP signaling components are expressed in human fracture callus.. Bone, [PMID:13678778]
4.Kang, Q Q and 14 more authors.. (2004) Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery.. Gene therapy, [PMID:15269709]
5.Chen, Andrew L AL and 5 more authors.. (2004) Expression of bone morphogenetic proteins, receptors, and tissue inhibitors in human fetal, adult, and osteoarthritic articular cartilage.. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, [PMID:15475196]
6.Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7):[PMID:15815621]
7.Allendorph, George P GP, Isaacs, Michael J MJ, Kawakami, Yasuhiko Y, Izpisua Belmonte, Juan Carlos JC and Choe, Senyon S.. (2007) BMP-3 and BMP-6 structures illuminate the nature of binding specificity with receptors.. Biochemistry, (30):[PMID:17924656]
8.Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16):[PMID:24275569]
9.Wen, Jialing and 9 more authors.. (2019) BMP3 suppresses colon tumorigenesis via ActRIIB/SMAD2-dependent and TAK1/JNK signaling pathways.. Journal of experimental & clinical cancer research : CR, (28):[PMID:31665064]
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