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SKU | Size | Availability | Price | Qty |
---|---|---|---|---|
rp144654-10μg | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $89.90 | |
rp144654-50μg | 50μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $249.90 | |
rp144654-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $399.90 | |
rp144654-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Product Name | Recombinant Human Carboxypeptidase E/CPE Protein |
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Synonyms | Carboxypeptidase H | CPE | CPH | EC 3.4.17.10 | Enkephalin convertase | Prohormone-processing carboxypeptidase | Carboxypeptidase H | Carboxypeptidase E | CPE | CPH | CPH-1 | EC 3.4.17 | EC 3.4.17.10 | Enkephalin convertase | Prohormone-processing carboxy |
Grade | ActiBioPure™, Animal Free, Bioactive, Carrier Free |
Product Description |
Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.
|
Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, ≥95%(SDS-PAGE) |
Bioactivity | Measured by its ability to cleave a peptide substrate, benzoyl-AR-OH. The product, Arg, is reacted with ortho-phthaldialdehyde (OPA) to form a fluorescent molecule. The specific activity is >12,000 pmol/min/µg, as measured under the described conditions. |
Endotoxin Concentration | <0.1 EU/μg |
Expression System | HEK293 |
Species | Human |
Amino Acids | 42-453 aa |
Sequence | RLQQEDGISFEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVHEPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNMKKIVDQNTKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGSAHEYSSSPDDAIFQSLARAYSSFNPAMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPPEETLKTYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVEGIDHDVTSAKDGDYWRLLIPGNYKLTASAPGYLAITKKVAVPYSPAAGVDFELESFSHHHHHH |
Protein Tag | C-His |
Accession # | P16870 |
Predicted molecular weight | 47 kDa |
SDS-PAGE | 57 kDa, under reducing conditions |
Form | Liquid |
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Reconstitution | Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml. |
Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
Shipped In | Ice chest + Ice pads |
Stability And Storage | Store at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle. |
1. Manser, E E and 6 more authors.. (1990) Human carboxypeptidase E. Isolation and characterization of the cDNA, sequence conservation, expression and processing in vitro.. The Biochemical journal, (15): [PMID:2334405] |
2. Utsunomiya, N N and 5 more authors.. (1998) Organization of the human carboxypeptidase E gene and molecular scanning for mutations in Japanese subjects with NIDDM or obesity.. Diabetologia, [PMID:9662053] |
3. Lee, Terence K TK and 13 more authors.. (2011) An N-terminal truncated carboxypeptidase E splice isoform induces tumor growth and is a biomarker for predicting future metastasis in human cancers.. The Journal of clinical investigation, [PMID:21285511] |
4. Liew, Chong Wee CW and 16 more authors.. (2014) Insulin regulates carboxypeptidase E by modulating translation initiation scaffolding protein eIF4G1 in pancreatic β cells.. Proceedings of the National Academy of Sciences of the United States of America, (3): [PMID:24843127] |
5. Lee, Terence K TK and 13 more authors.. (2019) An N-terminal truncated carboxypeptidase E splice isoform induces tumor growth and is a biomarker for predicting future metastasis in human cancers.. The Journal of clinical investigation, (18): [PMID:30882370] |
6. Durmaz, Asude and 9 more authors.. (2021) A New Cause of Obesity Syndrome Associated with a Mutation in the Carboxypeptidase Gene Detected in Three Siblings with Obesity, Intellectual Disability and Hypogonadotropic Hypogonadism. Journal of clinical research in pediatric endocrinology, (26): [PMID:32936766] |