| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp143727-10μg | 10μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $139.90 | |
| rp143727-50μg | 50μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $429.90 | |
| rp143727-100μg | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $669.90 | |
| rp143727-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Animal Free, >96%(SDS-PAGE and HPLC), Active, E.coli, No tag, 22-93 aa
| Product Name | Recombinant Human CCL14 Protein, ＞96%(SDS-PAGE and HPLC), high purity |
|---|---|
| Synonyms | CC-1; CC-3; CCL14; CCL14_HUMAN; chemokine (C-C motif) ligand 14; Chemokine CC-1/CC-3; chemokine CC1; chemokine CC3; chemokine HCC1; chemokine HCC3; CKb1; HCC 1; HCC 3; HCC-1(1-74); HCC-1(9-74); HCC-1/HCC-3; HCC-3; HCC1; HEMOFILTRATE CC CHEMOKINE 1; MCIF; |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance |
| Product Description | Purity > 96% by SDS-PAGE and HPLC analyses. Function Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. Post-translational The N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood. HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated; the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids. |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥96%(SDS-PAGE&HPLC) |
| Purity | ＞96%(SDS-PAGE and HPLC) |
| Bioactivity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5.0-20 ng/ml. |
| Endotoxin Concentration | ＜1.0 EU/μg |
| Expression System | E.coli |
| Species | Human |
| Amino Acids | 22-93 aa |
| Sequence | TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN |
| Protein Tag | No tag |
| Protein Length | Full length protein |
| Accession # | Q16627 |
| Source | Recombinant |
| Predicted molecular weight | 8.4 kDa |
Recombinant Human CCL14 Protein (rp143727)-Protein Bioactivity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 5-20 ng/mL.
Recombinant Human CCL14 Protein (rp143727)-SDS-PAGE
Recombinant Human CCL14 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a single band at 8.4 kDa.
| Form | Lyophilized |
|---|---|
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitu |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0600366 | Certificate of Analysis | Mar 26, 2024 | rp143727 |
| ZJ23F0600367 | Certificate of Analysis | Mar 26, 2024 | rp143727 |
| ZJ23F0600368 | Certificate of Analysis | Mar 26, 2024 | rp143727 |
| 1. Richter R, Schulz-Knappe P, John H, Forssmann WG. (2000) Posttranslationally processed forms of the human chemokine HCC-1.. Biochemistry, 39 (35): (10799-805). [PMID:10978165] |
| 2. Schulz-Knappe, P P and 16 more authors.. (1996) HCC-1, a novel chemokine from human plasma.. The Journal of experimental medicine, (1): [PMID:8551235] |
| 3. Pardigol, A A and 7 more authors.. (1998) HCC-2, a human chemokine: gene structure, expression pattern, and biological activity.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:9600961] |
| 4. Nomiyama, H H and 5 more authors.. (1999) Organization of the chemokine gene cluster on human chromosome 17q11.2 containing the genes for CC chemokine MPIF-1, HCC-2, HCC-1, LEC, and RANTES.. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, [PMID:10213461] |
| 5. Detheux, M M and 10 more authors.. (2000) Natural proteolytic processing of hemofiltrate CC chemokine 1 generates a potent CC chemokine receptor (CCR)1 and CCR5 agonist with anti-HIV properties.. The Journal of experimental medicine, (20): [PMID:11085751] |
| 6. Blain, Katherine Y KY and 14 more authors.. (2007) Structural and functional characterization of CC chemokine CCL14.. Biochemistry, (4): [PMID:17691823] |
