| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp148478-10μg | 10μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $59.90 | |
| rp148478-50μg | 50μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $249.90 | |
| rp148478-100μg | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $399.90 | |
| rp148478-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Animal Free, >97%(SDS-PAGE, HPLC), Active, E.coli, No tag, 22-113aa
| Product Name | Recombinant Human CCL15 Protein, ＞97%(SDS-PAGE, HPLC), high purityused for Active, SDS-PAGE various applications |
|---|---|
| Synonyms | CCL15 | chemokine (C-C motif) ligand 15 | Chemokine CC-2 | HCC-2 | HCC-2Lkn-1 | Leukotactin 1 | Leukotactin-1 | LKN1 | LKN-1 | Macrophage inflammatory protein 5 | MIP1 delta | MIP-1 delta | MIP5 | MIP-5 | MIP-5MIP-1d | Mrp-2b | NCC-3 | NCC-3MIP-1D | NCC3M |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance |
| Product Description | Purity > 97 % by SDS-PAGE and HPLC analyses. Function Human CCL15 is belonging to the CC chemokine family and shares 35 % amino acid homology with human HCC1 (CCL14). CCL15 is most abundant in heart, skeletal muscle and adrenal gland, and low expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15. |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE&HPLC) |
| Purity | ＞97%(SDS-PAGE, HPLC) |
| Bioactivity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml. |
| Endotoxin Concentration | <1.0 EU/μg |
| Expression System | E.coli |
| Species | Human |
| Amino Acids | 22-113 aa |
| Sequence | QFINDAETEL MMSKLPLENP VVLNSFHFAA DCCTSYISQS IPCSLMKSYF ETSSECSKPG VIFLTKKGRQ VCAKPSGPGV QDCMKKLKPY SI |
| Protein Tag | No tag |
| Protein Length | Full length protein |
| Accession # | Q16663 |
| Source | Recombinant |
| Predicted molecular weight | 10.2 kDa |
| SDS-PAGE | 10.2 kDa |
Recombinant Human CCL15 Protein (rp148478)-Protein Bioactivity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/mL.
Recombinant Human CCL15 Protein (rp148478)-SDS-PAGE
Recombinant Human CCL15 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a single band at 10.2 kDa.
| Application | Active, SDS-PAGE |
|---|---|
| Form | Lyophilized |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ℃ as supplied. 1 month, 2 to 8 ℃ under sterile conditions after reconstitution. 3 months, -20 to -70 ℃ under sterile conditions after reconstitu |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0600405 | Certificate of Analysis | Mar 26, 2024 | rp148478 |
| ZJ23F0600406 | Certificate of Analysis | Mar 26, 2024 | rp148478 |
| ZJ23F0600407 | Certificate of Analysis | Mar 26, 2024 | rp148478 |
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| 2. Youn, B S BS and 10 more authors.. (1997) Molecular cloning of leukotactin-1: a novel human beta-chemokine, a chemoattractant for neutrophils, monocytes, and lymphocytes, and a potent agonist at CC chemokine receptors 1 and 3.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:9548457] |
| 3. Youn, B S BS and 6 more authors.. (1998) Characterization of CKbeta8 and CKbeta8-1: two alternatively spliced forms of human beta-chemokine, chemoattractants for neutrophils, monocytes, and lymphocytes, and potent agonists at CC chemokine receptor 1.. Blood, (1): [PMID:9558365] |
| 4. Pardigol, A A and 7 more authors.. (1998) HCC-2, a human chemokine: gene structure, expression pattern, and biological activity.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:9600961] |
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| 6. Nomiyama, H H and 5 more authors.. (1999) Organization of the chemokine gene cluster on human chromosome 17q11.2 containing the genes for CC chemokine MPIF-1, HCC-2, HCC-1, LEC, and RANTES.. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, [PMID:10213461] |
| 7. Sticht, H H and 5 more authors.. (1999) Solution structure of the human CC chemokine 2: A monomeric representative of the CC chemokine subtype.. Biochemistry, (11): [PMID:10320325] |
| 8. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161] |
| 9. Berahovich, Robert D RD and 5 more authors.. (2005) Proteolytic activation of alternative CCR1 ligands in inflammation.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:15905581] |
