Recombinant Human CCL25 Protein, >97%(SDS-PAGE and HPLC), high purity

Features and benefits
  • Expression System: E.coli
  • Accession #: O15444
  • Protein Tag: No tag
  • Bioactivity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp143741
Grouped product items
SKUSizeAvailabilityPrice Qty
rp143741-10μg
10μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$69.90
rp143741-50μg
50μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$299.90
rp143741-100μg
100μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$499.90
rp143741-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Animal Free, >97%(SDS-PAGE and HPLC), Active, E.coli, No tag, 24-150 aa

Basic Description

Product NameRecombinant Human CCL25 Protein, >97%(SDS-PAGE and HPLC), high purity
SynonymsA130072A22Rik; C-C motif chemokine 25; CCL 25; CCL25; CCL25_HUMAN; Chemokine (C C motif) ligand 25; Chemokine (CC motif) ligand 25; Chemokine C C motif ligand 25; Chemokine CC motif ligand 25; Chemokine TECK; Ck beta 15; Ckb 15; Ckb15; MGC125074; MGC15032
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description

Purity
>97% by SDS-PAGE and HPLC analyses.

Function
Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE&HPLC)
Purity>97%(SDS-PAGE and HPLC)
BioactivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
Endotoxin Concentration<1.0 EU/μg
Expression SystemE.coli
SpeciesHuman
Amino Acids24-150 aa
SequenceMQGVFEDCCL AYHYPIGWAV LRRAWTYRIQ EVSGSCNLPA AIFYLPKRHR KVCGNPKSRE VQRAMKLLDA RNKVFAKLHH NTQTFQAGPH AVKKLSSGNS KLSSSKFSNP ISSSKRNVSL LISANSGL
Protein TagNo tag
Protein LengthFull length protein
Carrier FreeYes
Animal FreeYes
Accession #O15444
SourceRecombinant
Predicted molecular weight14.3 kDa
SDS-PAGE14.3 kDa

Images

Recombinant Human CCL25 Protein (rp143741)-SDS-PAGE
Recombinant Human CCL25 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining. Showing a single band at 15.5 kDa.

Recombinant Human CCL25 Protein (rp143741) - Protein Bioactivity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/mL.

Product Specifications

FormLyophilized
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconst

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ23F0700624Certificate of AnalysisJul 21, 2023 rp143741
ZJ23F0700623Certificate of AnalysisJul 20, 2023 rp143741
ZJ23F0700622Certificate of AnalysisJul 20, 2023 rp143741

Related Documents

References

1. Zaballos A, Gutiérrez J, Varona R, Ardavín C, Márquez G.  (1999)  Cutting edge: identification of the orphan chemokine receptor GPR-9-6 as CCR9, the receptor for the chemokine TECK..  J Immunol,  162  (10): (5671-5).  [PMID:10229797]
2. Watts AO, Verkaar F, van der Lee MM, Timmerman CA, Kuijer M, van Offenbeek J, van Lith LH, Smit MJ, Leurs R, Zaman GJ et al..  (2013)  β-Arrestin recruitment and G protein signaling by the atypical human chemokine decoy receptor CCX-CKR..  J Biol Chem,  288  (10): (7169-81).  [PMID:23341447]
3. Vicari, A P AP and 10 more authors..  (1997)  TECK: a novel CC chemokine specifically expressed by thymic dendritic cells and potentially involved in T cell development..  Immunity,      [PMID:9285413]
4. Grimwood, Jane J and 97 more authors..  (2004)  The DNA sequence and biology of human chromosome 19..  Nature,    (1):   [PMID:15057824]

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