| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp143746-10μg | 10μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $139.90 | |
| rp143746-50μg | 50μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $459.90 | |
| rp143746-100μg | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $739.90 | |
| rp143746-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Animal Free, >96%(SDS-PAGE, HPLC), Active, E.coli, No tag, 24-92 aa
| Product Name | Recombinant Human CCL4/MIP-1 beta Protein, ＞96%(SDS-PAGE, HPLC), high purity |
|---|---|
| Synonyms | ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance |
| Product Description | Purity > 96 % by SDS-PAGE and HPLC analyses. Function Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥96%(SDS-PAGE&HPLC) |
| Purity | ＞96%(SDS-PAGE, HPLC) |
| Bioactivity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5.0-20 ng/ml. |
| Endotoxin Concentration | ＜1.0 EU/μg |
| Expression System | E.coli |
| Species | Human |
| Amino Acids | 24-92 aa |
| Sequence | APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN |
| Protein Tag | No tag |
| Accession # | P13236 |
| Predicted molecular weight | 7.8 kDa |
Protein Bioactivity-Recombinant Human CCL4/MIP-1 beta Protein (rp143746)-Protein Bioactivity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5.0-20 ng/mL.
Recombinant Human CCL4/MIP-1 beta Protein (rp143746)-SDS-PAGE
Recombinant Human CCL4/MIP-1 beta Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 7.8 kDa.
| Form | Lyophilized |
|---|---|
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 ℃ as supplied. 1 month, 2 to 8 ℃ under sterile conditions after reconstitution. 3 months, -20 to -70 ℃ under sterile conditions after reconstitu |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0600408 | Certificate of Analysis | Mar 26, 2024 | rp143746 |
| ZJ23F0600409 | Certificate of Analysis | Mar 26, 2024 | rp143746 |
| ZJ23F0600410 | Certificate of Analysis | Mar 26, 2024 | rp143746 |
| 1. Bernardini G, Hedrick J, Sozzani S, Luini W, Spinetti G, Weiss M, Menon S, Zlotnik A, Mantovani A, Santoni A et al.. (1998) Identification of the CC chemokines TARC and macrophage inflammatory protein-1 beta as novel functional ligands for the CCR8 receptor.. Eur J Immunol, 28 (2): (582-8). [PMID:9521068] |
| 2. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334] |
| 3. Cocchi F, DeVico AL, Garzino-Demo A, Arya SK, Gallo RC, Lusso P. (1995) Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells.. Science, 270 (5243): (1811-5). [PMID:8525373] |
| 4. Napolitano, M M and 5 more authors.. (1991) The gene encoding the Act-2 cytokine. Genomic structure, HTLV-I/Tax responsiveness of 5' upstream sequences, and chromosomal localization.. The Journal of biological chemistry, (15): [PMID:1894635] |
| 5. Baixeras, E E and 7 more authors.. (1990) Cloning and expression of a lymphocyte activation gene (LAG-1).. Molecular immunology, [PMID:2247088] |
| 6. Lipes, M A MA, Napolitano, M M, Jeang, K T KT, Chang, N T NT and Leonard, W J WJ.. (1988) Identification, cloning, and characterization of an immune activation gene.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2462251] |
| 7. Brown, K D KD, Zurawski, S M SM, Mosmann, T R TR and Zurawski, G G.. (1989) A family of small inducible proteins secreted by leukocytes are members of a new superfamily that includes leukocyte and fibroblast-derived inflammatory agents, growth factors, and indicators of various activation processes.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:2521353] |
| 8. Zipfel, P F PF, Balke, J J, Irving, S G SG, Kelly, K K and Siebenlist, U U.. (1989) Mitogenic activation of human T cells induces two closely related genes which share structural similarities with a new family of secreted factors.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:2521882] |
| 9. Chang, H C HC and Reinherz, E L EL.. (1989) Isolation and characterization of a cDNA encoding a putative cytokine which is induced by stimulation via the CD2 structure on human T lymphocytes.. European journal of immunology, [PMID:2568930] |
| 10. Miller, M D MD, Hata, S S, De Waal Malefyt, R R and Krangel, M S MS.. (1989) A novel polypeptide secreted by activated human T lymphocytes.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:2809212] |
| 11. Lodi, P J PJ and 7 more authors.. (1994) High-resolution solution structure of the beta chemokine hMIP-1 beta by multidimensional NMR.. Science (New York, N.Y.), (25): [PMID:8134838] |
| 12. Garlisi, C G CG and 6 more authors.. (1999) The assignment of chemokine-chemokine receptor pairs: TARC and MIP-1 beta are not ligands for human CC-chemokine receptor 8.. European journal of immunology, [PMID:10540332] |
| 13. Guan, Ennan E, Wang, Jinhai J, Roderiquez, Gregory G and Norcross, Michael A MA.. (2002) Natural truncation of the chemokine MIP-1 beta /CCL4 affects receptor specificity but not anti-HIV-1 activity.. The Journal of biological chemistry, (30): [PMID:12070155] |
| 14. Menten, Patricia P, Wuyts, Anja A and Van Damme, Jo J.. (2002) Macrophage inflammatory protein-1.. Cytokine & growth factor reviews, [PMID:12401480] |
| 15. Colobran, Roger R and 7 more authors.. (2005) Multiple products derived from two CCL4 loci: high incidence of a new polymorphism in HIV+ patients.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:15843566] |
| 16. Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20): [PMID:16625196] |
