Bioactivity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
Endotoxin Concentration: <0.1 EU/μg
Item Number
rp143752
Grouped product items
SKU
Size
Availability
Price
Qty
rp143752-10μg
10μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$69.90
rp143752-50μg
50μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$299.90
rp143752-100μg
100μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$499.90
rp143752-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description
Purity: >97%(SDS-PAGE, HPLC)
Function: Chemokine that induces chemotaxis of cells expressing CCR5 or CCR1. Inhibits HIV replication in peripheral blood monocytes that express CCR5.
Background: CCL4, also known as MIP-1 beta, is a 12 kDa beta chemokine that is secreted by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. CCL4 interacts with CCR5 to attract lymphocytes, NK cells, and immature dendritic cells to sites of inflammation. CCL4 also blocks the entry of HIV into CCR5-expressing cells. Human CCL4 and its variants are encoded by two paralogous genes, one of which (SCYA4) encodes CCL4 itself. The other gene (SCYA4L) is found in two alleles that give rise to CCL4L1 and CCL4L2, respectively. The human CCL4L1 cDNA encodes a 92 amino acid (aa) precursor with a 23 aa signal sequence. Alternate splicing yields an isoform with a 40 aa internal deletion. The second allele (SCYA4L2), encoding CCL4L2, is spliced into a variety of isoforms. Human CC4L1 shares greater than 98% aa sequence identity with CCL4 and CCL4L2. It shares 96% aa sequence identity with rhesus CCL4 and approximately 80 - 90% aa sequence identity with bovine, mouse, rabbit, and rat CCL4. The gene copy number for CCL4L1 varies from zero to five, but this is not associated with changes in mRNA or protein levels. CCL4 and CCL4L1 demonstrate comparable CCR5 binding, promotion of chemotaxis, and inhibition of HIV replication in stimulated PBMC. In vivo, the first two N-terminal amino acids of CCL4 are removed by DPPIV. The truncated form is as active as full-length CCL4 and gains the ability to bind CCR1 and CCR2b. Similar proteolytic processing of CCL4L1 has not been described.
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
Recombinant Human CCL4L1 Protein (rp143752)-Protein Bioactivity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0 ng/mL, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.
Recombinant Human CCL4L1 Protein (rp143752)-SDS-PAGE Recombinant Human CCL4L1 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining. Showing a single band at 7.8 kDa.
Product Specifications
Form
Lyophilized
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 1.0 mg/mL. Stock solutions should be apportioned
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Certificates
Certificate of Analysis(COA)
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1.Modi, W S WS, Bergeron, J J and Sanford, M M.. (2001) The human MIP-1beta chemokine is encoded by two paralogous genes, ACT-2 and LAG-1.. Immunogenetics, [PMID:11685466]
2.Lu, Jun J, Honczarenko, Marek M and Sloan, Steven R SR.. (2004) Independent expression of the two paralogous CCL4 genes in monocytes and B lymphocytes.. Immunogenetics, [PMID:14673550]
3. and Modi, William S WS.. (2004) CCL3L1 and CCL4L1 chemokine genes are located in a segmental duplication at chromosome 17q12.. Genomics, [PMID:15028295]
4.Howard, O M Zack OM, Turpin, Jim A JA, Goldman, Robert R and Modi, William S WS.. (2004) Functional redundancy of the human CCL4 and CCL4L1 chemokine genes.. Biochemical and biophysical research communications, (30):[PMID:15240137]
5.Colobran, Roger R and 7 more authors.. (2005) Multiple products derived from two CCL4 loci: high incidence of a new polymorphism in HIV+ patients.. Journal of immunology (Baltimore, Md. : 1950), (1):[PMID:15843566]
6.Shao, W W and 6 more authors.. (2007) CCL3L1 and CCL4L1: variable gene copy number in adolescents with and without human immunodeficiency virus type 1 (HIV-1) infection.. Genes and immunity, [PMID:17330138]
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