Bioactivity: Measured by its binding ability in a functional ELISA. Immobilized human CD34 (rp156652) at 2.0 μg/mL can bind Mouse CD34 mAb (Ab094638) with the EC₅₀ of 31.41 ng/mL.
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10μg
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$139.90
rp156652-50μg
50μg
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$309.90
rp156652-100μg
100μg
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$499.90
rp156652-1mg
1mg
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Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining Description: CD34 is a 115 kDa glycosylated type I transmembrane protein; it was discovered as a hematopoietic cell-surface antigen. Human CD34 cDNA encodes a 385 amino acid (aa) precursor that contains a 31 aa signal sequence, a 259 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 74 aa cytoplasmic domain. Within the ECD, human CD34 shares 55% and 52% aa sequence identity with mouse and rat CD34, respectively. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein Kinase C (PKC). CD34 is found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines, and it is a common marker for diverse progenitors. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.
Measured by its binding ability in a functional ELISA. Immobilized human CD34 (rp156652) at 2.0 μg/mL can bind Mouse CD34 mAb (Ab094638) with the EC₅₀ of 31.41 ng/mL.
96.5-131.2 kDa, under reducing conditions; 96.5-131.2 kDa, under non-reducing condition
Images
Recombinant Human CD34 Protein (rp156652) - SDS-PAGE 3 μg/lane of Recombinant Human CD34 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 96.5-131.2 kDa.
Recombinant Human CD34 Protein (rp156652) - ELISA Measured by its binding ability in a functional ELISA. Immobilized human CD34 (rp156652) at 2.0 μg/mL can bind Mouse CD34 mAb (Ab094638) with the EC₅₀ of 31.41 ng/mL.
Product Specifications
Form
Lyophilized
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C stable up to 1 year. Avoid freeze / thaw cycle.
Certificates
Certificate of Analysis(COA)
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