Recombinant Human CD34 Protein, >95% (SDS-PAGE), high purity

Features and benefits
  • Expression System: HEK293
  • Protein Tag: C-His
  • Bioactivity: Measured by its binding ability in a functional ELISA. Immobilized human CD34 (rp156652) at 2.0 μg/mL can bind Mouse CD34 mAb (Ab094638) with the EC₅₀ of 31.41 ng/mL.
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp156652
Grouped product items
SKUSizeAvailabilityPrice Qty
rp156652-10μg (Trial Size)
Apply for free trial size(?)
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10μg
In stock
$139.90
rp156652-50μg
50μg
In stock
$309.90
rp156652-100μg
100μg
In stock
$499.90
rp156652-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Animal Free, >95% (SDS-PAGE), Active, 293F cell, C-His tag, 1-290 aa

Basic Description

Product NameRecombinant Human CD34 Protein, >95% (SDS-PAGE), high purity
SynonymsCD34 | CD34 antigen | CD34 molecule | CD34_HUMAN | Cluster designation 34 | cluster of differentiation 34 | Hematopoietic progenitor cell antigen CD34 | HPCA1 | Mucosialin | OTTHUMP00000034733 | OTTHUMP00000034734
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description

Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining
Description:
CD34 is a 115 kDa glycosylated type I transmembrane protein; it was discovered as a hematopoietic cell-surface antigen. Human CD34 cDNA encodes a 385 amino acid (aa) precursor that contains a 31 aa signal sequence, a 259 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 74 aa cytoplasmic domain. Within the ECD, human CD34 shares 55% and 52% aa sequence identity with mouse and rat CD34, respectively. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein Kinase C (PKC). CD34 is found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines, and it is a common marker for diverse progenitors. CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
Purity>95% (SDS-PAGE)
BioactivityMeasured by its binding ability in a functional ELISA. Immobilized human CD34 (rp156652) at 2.0 μg/mL can bind Mouse CD34 mAb (Ab094638) with the EC₅₀ of 31.41 ng/mL.
Endotoxin Concentration<1.0 EU/μg
Expression SystemHEK293
Amino Acids1-290 aa
SequenceMLVRRGARAGPRMPRGWTALCLLSLLPSGFMSLDNNGTATPELPTQGTFSNVSTNVSYQETTTPSTLGSTSLHPVSQHGNEATTNITETTVKFTSTSVITSVYGNTNSSVQSQTSVISTVFTTPANVSTPETTLKPSLSPGNVSDLSTTSTSLATSPTKPYTSSSPILSDIKAEIKCSGIREVKLTQGICLEQNKTSSCAEFKKDRGEGLARVLCGEEQADADAGAQVCSLLLAQSEVRPQCLLLVLANRTEISS
Protein TagC-His
Predicted molecular weight27.5 kDa
SDS-PAGE96.5-131.2 kDa, under reducing conditions; 96.5-131.2 kDa, under non-reducing condition

Images

Recombinant Human CD34 Protein (rp156652) - SDS-PAGE
3 μg/lane of Recombinant Human CD34 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 96.5-131.2 kDa.

Recombinant Human CD34 Protein (rp156652) - ELISA
Measured by its binding ability in a functional ELISA. Immobilized human CD34 (rp156652) at 2.0 μg/mL can bind Mouse CD34 mAb (Ab094638) with the EC₅₀ of 31.41 ng/mL.

Product Specifications

FormLyophilized
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C stable up to 1 year. Avoid freeze / thaw cycle.

Certificates

Certificate of Analysis(COA)

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4 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303253Certificate of AnalysisMar 18, 2024 rp156652
ZJ24F0303254Certificate of AnalysisMar 18, 2024 rp156652
ZJ24F0303255Certificate of AnalysisMar 18, 2024 rp156652
ZJ24R0300325Certificate of AnalysisMar 08, 2024 rp156652

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