| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp152636-10μg | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| rp152636-50μg | 50μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $139.90 | |
| rp152636-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $229.90 | |
| rp152636-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,989.90 |
Animal Free, >95% (SDS-PAGE&HPLC), Active, E.coli, No tag,113-263 aa
| Product Name | Recombinant Human CD40 Ligand/TNFSF5 Protein, >95% (SDS-PAGE&HPLC), high purity |
|---|---|
| Synonyms | CD154 antigen; CD154; CD40 antigen ligand; CD40 Ligand; CD40L; CD40-L; CD40LG; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TNFSF5IMD3; TRAP; TRAPtumor necrosis factor (li |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free |
| Product Description | Purity |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE&HPLC) |
| Purity | >95% (SDS-PAGE&HPLC) |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/ml. |
| Endotoxin Concentration | <1.0 EU/μg |
| Expression System | E.coli |
| Species | Human |
| Amino Acids | 113-263 aa |
| Sequence | MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLWLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL |
| Protein Tag | No tag |
| Accession # | P29965 |
| Predicted molecular weight | 16.3 kDa |
| SDS-PAGE | 16.3 kDa |
Recombinant Human CD40 Ligand/TNFSF5 Protein (rp152636) - Protein Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/mL.
Recombinant Human CD40 Ligand/TNFSF5 Protein (rp152636) - SDS-PAGE
Recombinant Human CD40 Ligand/TNFSF5 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining. Showing a single band at 16.3 kDa.
| Form | Lyophilized |
|---|---|
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-0.3 mg/mL. Stock solutions should be apportio |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. Upon delivery aliquot. Avoid freeze/thaw cycle. |
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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1001351 | Certificate of Analysis | Oct 19, 2023 | rp152636 |
| ZJ23F1001352 | Certificate of Analysis | Oct 19, 2023 | rp152636 |
| ZJ23F1001353 | Certificate of Analysis | Oct 19, 2023 | rp152636 |
| 1. Chen J, Song Y, Bojadzic D, Tamayo-Garcia A, Landin AM, Blomberg BB, Buchwald P.. (2017) Small-Molecule Inhibitors of the CD40-CD40L Costimulatory Protein-Protein Interaction.. J Med Chem, 60 (21): (8906-8922). [PMID:29024591] |
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| 7. Gauchat, J F JF and 6 more authors.. (1993) Human CD40-ligand: molecular cloning, cellular distribution and regulation of expression by factors controlling IgE production.. FEBS letters, (11): [PMID:7678552] |
| 8. Aruffo, A A and 9 more authors.. (1993) The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome.. Cell, (29): [PMID:7678782] |
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| 10. Allen, R C RC and 9 more authors.. (1993) CD40 ligand gene defects responsible for X-linked hyper-IgM syndrome.. Science (New York, N.Y.), (12): [PMID:7679801] |
| 11. Macchi, P P and 9 more authors.. (1995) Characterization of nine novel mutations in the CD40 ligand gene in patients with X-linked hyper IgM syndrome of various ancestry.. American journal of human genetics, [PMID:7717401] |
| 12. Shimadzu, M M and 6 more authors.. (1995) Structural organization of the gene for CD40 ligand: molecular analysis for diagnosis of X-linked hyper-IgM syndrome.. Biochimica et biophysica acta, (2): [PMID:7999797] |
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| 14. Lin, Q Q and 9 more authors.. (1996) A single strand conformation polymorphism study of CD40 ligand. Efficient mutation analysis and carrier detection for X-linked hyper IgM syndrome.. The Journal of clinical investigation, (1): [PMID:8550833] |
| 15. Karpusas, M M and 6 more authors.. (1995) 2 A crystal structure of an extracellular fragment of human CD40 ligand.. Structure (London, England : 1993), (15): [PMID:8589998] |
| 16. Blotta, M H MH, Marshall, J D JD, DeKruyff, R H RH and Umetsu, D T DT.. (1996) Cross-linking of the CD40 ligand on human CD4+ T lymphocytes generates a costimulatory signal that up-regulates IL-4 synthesis.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:8617933] |
| 17. Pietravalle, F F and 7 more authors.. (1996) Human native soluble CD40L is a biologically active trimer, processed inside microsomes.. The Journal of biological chemistry, (15): [PMID:8626375] |
| 18. Katz, F F and 5 more authors.. (1996) Mutation analysis in CD40 ligand deficiency leading to X-linked hypogammaglobulinemia with hyper IgM syndrome.. Human mutation, [PMID:8889581] |
| 19. Nonoyama, S S and 7 more authors.. (1997) Mutations of the CD40 ligand gene in 13 Japanese patients with X-linked hyper-IgM syndrome.. Human genetics, [PMID:9150729] |
| 20. Singh, J J and 7 more authors.. (1998) The role of polar interactions in the molecular recognition of CD40L with its receptor CD40.. Protein science : a publication of the Protein Society, [PMID:9605317] |
| 21. Seyama, K K and 6 more authors.. (1998) Mutations of the CD40 ligand gene and its effect on CD40 ligand expression in patients with X-linked hyper IgM syndrome.. Blood, (1): [PMID:9746782] |
| 22. Khandekar, S S SS and 9 more authors.. (2001) Determination of carbohydrate structures N-linked to soluble CD154 and characterization of the interactions of CD40 with CD154 expressed in Pichia pastoris and Chinese hamster ovary cells.. Protein expression and purification, [PMID:11676606] |
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