| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp144000-10μg | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $109.90 | |
| rp144000-50μg | 50μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $349.90 | |
| rp144000-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $529.90 | |
| rp144000-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $3,429.90 |
Carrier Free, >95%(SDS-PAGE), Active, HEK293, C-Fc tag, 21-193 aa
| Product Name | Recombinant Human CD40 Protein, ＞95%(SDS-PAGE), high purity |
|---|---|
| Synonyms | AI326936; B cell associated molecule CD40; B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD 40; CD40; CD40 antigen; CD40 antigen (TNF receptor superfamily member 5); CD40 molecule; CD40 molecule, TNF receptor |
| Grade | ActiBioPure™, Azide Free, Bioactive, Carrier Free |
| Product Description | Purity |
| Specifications & Purity | ActiBioPure™, Bioactive, Carrier Free, Azide Free, ≥95%(SDS-PAGE) |
| Purity | ＞95%(SDS-PAGE) |
| Bioactivity | Immobilized human CD40L-His at 10 μg/mL (100 μL/well) can bind Human CD40-Fc. The EC50 of Human CD40-Fc is 10-30 ng/mL. |
| Endotoxin Concentration | <1.0 EU/μg |
| Expression System | HEK293 |
| Species | Human |
| Amino Acids | 21-193 aa |
| Sequence | EPPTACREKQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCGESEFLDTWNRETHCHQHKYCDPNLGLRVQQKGTSETDTICTCEEGWHCTSEACESCVLHRSCSPGFGVKQIATGVSDTICEPCPVGFFSNVSSAFEKCHPWTSCETKDLVVQQAGTNKTDVVCGPQDRLRALEVLFQGPEPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAK |
| Protein Tag | C-Fc |
| Accession # | P25942 |
| Predicted molecular weight | 46.2 kDa |
| SDS-PAGE | 54.1 kDa, reducing conditions |
Recombinant Human CD40 Protein (rp144000) - Protein Bioactivity
Immobilized Recombinant Human CD40L/CD154/TNFSF5 Protein at 2.0 μg/mL can bind Recombinant Human CD40 Protein (rp144000), the EC₅₀ is 10 - 30 ng/mL.
Recombinant Human CD40 Protein (rp144000) - SDS-PAGE
3 μg/lane of Recombinant Human CD40 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 57.5 kDa under reducing conditions and 113.9 kDa under non-reducing conditions.
Recombinant Human CD40 Protein (rp144000) - ELISA
Immobilized Recombinant Human CD40 Protein (rp144000) at 1.0 μg/mL can bind Teneliximab (anti-CD40) (Ab183440) with the EC50 of 11.50 ng/mL.
| Form | Lyophilized |
|---|---|
| Reconstitution | It is recommended that sterile water (1100ul) be added to the vial to prepare a stock solution of 0.91mg/mL. |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Samples are stable for twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0505380 | Certificate of Analysis | May 15, 2024 | rp144000 |
| ZJ24F0505379 | Certificate of Analysis | May 15, 2024 | rp144000 |
| ZJ24F0505378 | Certificate of Analysis | May 15, 2024 | rp144000 |
| 1. Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J. (2001) The DNA sequence and comparative analysis of human chromosome 20.. Nature, 414 (6866): (865-71). [PMID:11780052] |
| 2. Chen J, Song Y, Bojadzic D, Tamayo-Garcia A, Landin AM, Blomberg BB, Buchwald P.. (2017) Small-Molecule Inhibitors of the CD40-CD40L Costimulatory Protein-Protein Interaction.. J Med Chem, 60 (21): (8906-8922). [PMID:29024591] |
| 3. Braesch-Andersen, S S and 5 more authors.. (1989) Biochemical characteristics and partial amino acid sequence of the receptor-like human B cell and carcinoma antigen CDw40.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:2463309] |
| 4. Stamenkovic, I I, Clark, E A EA and Seed, B B.. (1989) A B-lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinomas.. The EMBO journal, [PMID:2475341] |
| 5. Sato, T T, Irie, S S and Reed, J C JC.. (1995) A novel member of the TRAF family of putative signal transducing proteins binds to the cytosolic domain of CD40.. FEBS letters, (23): [PMID:7530216] |
| 6. Cheng, G G and 5 more authors.. (1995) Involvement of CRAF1, a relative of TRAF, in CD40 signaling.. Science (New York, N.Y.), (10): [PMID:7533327] |
| 7. Bajorath, J J and Aruffo, A A.. (1997) Construction and analysis of a detailed three-dimensional model of the ligand binding domain of the human B cell receptor CD40.. Proteins, [PMID:9037712] |
| 8. Kashiwada, M M and 8 more authors.. (1998) Tumor necrosis factor receptor-associated factor 6 (TRAF6) stimulates extracellular signal-regulated kinase (ERK) activity in CD40 signaling along a ras-independent pathway.. The Journal of experimental medicine, (19): [PMID:9432981] |
| 9. Mizushima, S S and 8 more authors.. (1998) Cloning and characterization of a cDNA encoding the human homolog of tumor necrosis factor receptor-associated factor 5 (TRAF5).. Gene, (30): [PMID:9511754] |
| 10. Singh, J J and 7 more authors.. (1998) The role of polar interactions in the molecular recognition of CD40L with its receptor CD40.. Protein science : a publication of the Protein Society, [PMID:9605317] |
| 11. Pullen, S S SS and 5 more authors.. (1998) CD40-tumor necrosis factor receptor-associated factor (TRAF) interactions: regulation of CD40 signaling through multiple TRAF binding sites and TRAF hetero-oligomerization.. Biochemistry, (25): [PMID:9718306] |
| 12. Ni, C Z CZ and 6 more authors.. (2000) Molecular basis for CD40 signaling mediated by TRAF3.. Proceedings of the National Academy of Sciences of the United States of America, (12): [PMID:10984535] |
| 13. Tone, M M, Tone, Y Y, Fairchild, P J PJ, Wykes, M M and Waldmann, H H.. (2001) Regulation of CD40 function by its isoforms generated through alternative splicing.. Proceedings of the National Academy of Sciences of the United States of America, (13): [PMID:11172023] |
| 14. Ferrari, S S and 15 more authors.. (2001) Mutations of CD40 gene cause an autosomal recessive form of immunodeficiency with hyper IgM.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:11675497] |
| 15. Khandekar, S S SS and 9 more authors.. (2001) Determination of carbohydrate structures N-linked to soluble CD154 and characterization of the interactions of CD40 with CD154 expressed in Pichia pastoris and Chinese hamster ovary cells.. Protein expression and purification, [PMID:11676606] |
| 16. Li, Chenglong C and 9 more authors.. (2002) Downstream regulator TANK binds to the CD40 recognition site on TRAF3.. Structure (London, England : 1993), [PMID:12005438] |
| 17. Tai, Yu-Tzu YT and 13 more authors.. (2004) Mechanisms by which SGN-40, a humanized anti-CD40 antibody, induces cytotoxicity in human multiple myeloma cells: clinical implications.. Cancer research, (15): [PMID:15087402] |
| 18. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161] |
| 19. Ouadani, Hanen H and 14 more authors.. (2016) Novel and recurrent AID mutations underlie prevalent autosomal recessive form of HIGM in consanguineous patients.. Immunogenetics, [PMID:26545377] |
| 20. Takada, Yoko K; Yu, Jessica; Shimoda, Michiko and Takada, Yoshikazu.. (2019) Integrin Binding to the Trimeric Interface of CD40L Plays a Critical Role in CD40/CD40L Signaling.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:31331973] |
