Recombinant Human CD47 Protein, >95% (SDS-PAGE), high purity

Features and benefits
  • Expression System: HEK293
  • Accession #: Q08722
  • Protein Tag: C-Fc
  • Bioactivity: 1. Measured by its binding ability in a functional ELISA. Immobilized human B7-H7/HHLA2 Protein at 10 μg/mL can bind Recombinant Human CD47 Protein (rp144028), the EC50 of human CD47 Protein is 50.0 - 200.0 ng/mL. 2. Measured by its binding ability in a f
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp144028
Grouped product items
SKUSizeAvailabilityPrice Qty
rp144028-10μg
10μg
In stock
$599.90
rp144028-50μg
50μg
In stock
$1,549.90
rp144028-100μg
100μg
In stock
$2,479.90
rp144028-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$15,999.90

Animal Free, >95% (SDS-PAGE), Active, HEK293, C-Fc tag, 19-139 aa

Basic Description

Product NameRecombinant Human CD47 Protein, >95% (SDS-PAGE), high purity
Synonymsantigen identified by monoclonal 1D8; Antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47; IAP; IAPintegrin associated protein; Integrin-
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description

Purity
>95% (SDS-PAGE)
Endotoxin level
<1.0 EU/μg
Function
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
Purity>95% (SDS-PAGE)
Bioactivity1. Measured by its binding ability in a functional ELISA. Immobilized human B7-H7/HHLA2 Protein at 10 μg/mL can bind Recombinant Human CD47 Protein (rp144028), the EC50 of human CD47 Protein is 50.0 - 200.0 ng/mL. 2. Measured by its binding ability in a f
Endotoxin Concentration<1.0 EU/μg
Expression SystemHEK293
SpeciesHuman
Amino Acids19-139 aa
SequenceQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPALEVLFQGPEPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAK
Protein TagC-Fc
Accession #Q08722
Predicted molecular weight40.7 kDa
SDS-PAGE59.3 kDa, under reducing conditions; 109.3 kDa, under non-reducing conditions.

Images

Recombinant Human CD47 Protein (rp144028) - Protein Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized human B7-H7/HHLA2 Protein at 10 μg/mL can bind Recombinant Human CD47 Protein (rp144028), the EC₅₀ of human CD47 Protein is 50.0 - 200.0 ng/mL.

Recombinant Human CD47 Protein (rp144028) - ELISA
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human CD47 Protein (rp144028) at 1 μg/mL can bind Magrolimab (anti-CD47) (Ab169323) with the EC₅₀ of 10.34 ng/mL. 

Recombinant Human CD47 Protein (rp144028) - SDS-PAGE
3 μg/lane of Recombinant Human CD47 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 59.3 kDa under reducing conditions and 109.3 kDa under non-reducing conditions.

Product Specifications

FormLyophilized
ReconstitutionIt is recommended that sterile water be added to the vial to prepare a stock solution of 0.25mg/mL.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageSamples are stable for twelve months from date of receipt at -20℃ to -80℃. Upon delivery aliquot. Avoid freeze/thaw cycle.

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ23F1101861Certificate of AnalysisDec 01, 2023 rp144028
ZJ23F1101860Certificate of AnalysisDec 01, 2023 rp144028
ZJ23F1101859Certificate of AnalysisDec 01, 2023 rp144028

Related Documents

References

1. Kauder SE, Kuo TC, Harrabi O, Chen A, Sangalang E, Doyle L, Rocha SS, Bollini S, Han B, Sim J et al..  (2018)  ALX148 blocks CD47 and enhances innate and adaptive antitumor immunity with a favorable safety profile..  PLoS One,  13  (8): (e0201832).  [PMID:30133535]
2. Petrova PS, Viller NN, Wong M, Pang X, Lin GH, Dodge K, Chai V, Chen H, Lee V, House V et al..  (2017)  TTI-621 (SIRPαFc): A CD47-Blocking Innate Immune Checkpoint Inhibitor with Broad Antitumor Activity and Minimal Erythrocyte Binding..  Clin Cancer Res,  23  (4): (1068-1079).  [PMID:27856600]
3. Campbell, I G IG, Freemont, P S PS, Foulkes, W W and Trowsdale, J J..  (1992)  An ovarian tumor marker with homology to vaccinia virus contains an IgV-like region and multiple transmembrane domains..  Cancer research,    (1):   [PMID:1394148]
4. Lindberg, F P FP, Gresham, H D HD, Schwarz, E E and Brown, E J EJ..  (1993)  Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane-spanning domains implicated in alpha v beta 3-dependent ligand binding..  The Journal of cell biology,      [PMID:7691831]
5. Mawby, W J WJ, Holmes, C H CH, Anstee, D J DJ, Spring, F A FA and Tanner, M J MJ..  (1994)  Isolation and characterization of CD47 glycoprotein: a multispanning membrane protein which is the same as integrin-associated protein (IAP) and the ovarian tumour marker OA3..  The Biochemical journal,    (1):   [PMID:7998989]
6. Gao, A G AG and 5 more authors..  (1996)  Integrin-associated protein is a receptor for the C-terminal domain of thrombospondin..  The Journal of biological chemistry,    (5):   [PMID:8550562]
7. Wu, A L AL, Wang, J J, Zheleznyak, A A and Brown, E J EJ..  (1999)  Ubiquitin-related proteins regulate interaction of vimentin intermediate filaments with the plasma membrane..  Molecular cell,      [PMID:10549293]
8. Rebres, R A RA, Vaz, L E LE, Green, J M JM and Brown, E J EJ..  (2001)  Normal ligand binding and signaling by CD47 (integrin-associated protein) requires a long range disulfide bond between the extracellular and membrane-spanning domains..  The Journal of biological chemistry,    (14):   [PMID:11454874]
9. Latour, S S and 11 more authors..  (2001)  Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:11509594]
10. Piccio, Laura L and 7 more authors..  (2005)  Adhesion of human T cells to antigen-presenting cells through SIRPbeta2-CD47 interaction costimulates T-cell proliferation..  Blood,    (15):   [PMID:15383453]
11. Manna, Partha P PP, Dimitry, Julie J, Oldenborg, Per-Arne PA and Frazier, William A WA..  (2005)  CD47 augments Fas/CD95-mediated apoptosis..  The Journal of biological chemistry,    (19):   [PMID:15917238]
12. Hatherley, Deborah D and 5 more authors..  (2008)  Paired receptor specificity explained by structures of signal regulatory proteins alone and complexed with CD47..  Molecular cell,    (25):   [PMID:18657508]
13. Wollscheid, Bernd B and 7 more authors..  (2009)  Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins..  Nature biotechnology,      [PMID:19349973]
14. De Oliveira, S S, Vitorino de Almeida, V V, Calado, A A, Rosário, H S HS and Saldanha, C C..  (2012)  Integrin-associated protein (CD47) is a putative mediator for soluble fibrinogen interaction with human red blood cells membrane..  Biochimica et biophysica acta,      [PMID:22079249]
15. Rogers, Natasha M NM and 24 more authors..  (2017)  TSP1-CD47 signaling is upregulated in clinical pulmonary hypertension and contributes to pulmonary arterial vasculopathy and dysfunction..  Cardiovascular research,      [PMID:27742621]
16. Ghimire, Kedar and 11 more authors..  (2020)  CD47 Promotes Age-Associated Deterioration in Angiogenesis, Blood Flow and Glucose Homeostasis..  Cells,    (15):   [PMID:32679764]

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