| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp184121-10μg (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $139.90 | |
| rp184121-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $699.90 | |
| rp184121-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $4,599.90 |
Carrier Free, ≥90% (SDS-PAGE), E. coli, N-His tag, 2-303 aa
| Product Name | Recombinant Human CDK4 Protein |
|---|---|
| Synonyms | cdk4 | CDK4 protein | CDK4_HUMAN | Cell division kinase 4 | Cell division protein kinase 4 | CMM 3 | CMM3 | Crk3 | Cyclin dependent kinase 4 | Cyclin-dependent kinase 4 | Melanoma cutaneous malignant 3 | MGC14458 | p34 cdk4 | Cdk 4 | PSK J3 | PSK-J3 |
| Grade | Carrier Free |
| Product Description |
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
|
| Specifications & Purity | Carrier Free, ≥90%(SDS-PAGE) |
| Endotoxin Concentration | ＜1.0 EU/μg |
| Expression System | E. coli |
| Amino Acids | 2-303 aa |
| Sequence | MHHHHHHATSRYEPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVPNGGGGGGGLPISTVREVALLRRLEAFEHPNVVRLMDVCATSRTDREIKVTLVFEHVDQDLRTYLDKAPPPGLPAETIKDLMRQFLRGLDFLHANCIVHRDLKPENILVTSGGTVKLADFGLARIYSYQMALTPVVVTLWYRAPEVLLQSTYATPVDMWSVGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAF |
| Protein Tag | N-His |
| Predicted molecular weight | 34.5 kDa |
| SDS-PAGE | 32.0 kDa, under reducing conditions; 32.0 kDa, under non-reducing conditions |
Recombinant Human CDK4 Protein (rp184121) - SDS-PAGE
2 μg/lane of Recombinant Human CDK4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 32.0 kDa.
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu |
|---|---|
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C for 1 year. Avoid freeze / thaw cycle. |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0707743 | Certificate of Analysis | Jul 05, 2024 | rp184121 |
| ZJ24F0707742 | Certificate of Analysis | Jul 05, 2024 | rp184121 |
| ZJ24R0600704 | Certificate of Analysis | Jun 23, 2024 | rp184121 |
