Recombinant Human Cofilin Protein, >90%(SDS-PAGE), high purity

Features and benefits
  • Expression System: E. coli
  • Protein Tag: N-His
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp169589
Grouped product items
SKUSizeAvailabilityPrice Qty
rp169589-10μg (Trial Size)
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10μg
In stock
$99.90
rp169589-100μg
100μg
In stock
$329.90
rp169589-1mg
1mg
In stock
$1,799.90

Carrier Free, >90%(SDS-PAGE), E.coli, His tag, 1-166 aa

Basic Description

Product NameRecombinant Human Cofilin Protein, >90%(SDS-PAGE), high purity
SynonymsCFL1; Cofilin 1; CFL; Cofilin 1 (Non-Muscle); 18 KDa Phosphoprotein; Cofilin-1; P18; Epididymis Secretory Protein Li 15; Cofilin; Non-Muscle Isoform; HEL-S-15; Cofilin
GradeAzide Free, Carrier Free
Product Description

Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.

Description:

CFL1, also known as n-cofilin, is a member of the ADF/Cofilin family. This family comprises three genes: CFL1, CFL2 and DSTN (destrin). ADF/Cofilin family members bind G-actin monomers and depolymerize actin filaments through two mechanisms: severing and increasing the off-rate for actin monomers from the pointed end. Cofilin also binds with other proteins such as myosin, tropomyosin, α-actinin, gelsolin and scruin. These proteins compete with cofilin for actin binding. Сofilin also plays a role in innate immune response. CFL1 contains 1 ADF-H domain and is widely distributed in various tissues. It is important for normal progress through mitosis and normal cytokinesis.


Specifications & PurityCarrier Free, Azide Free, ≥90%(SDS-PAGE)
Purity>90%(SDS-PAGE)
Endotoxin Concentration<1.0 EU/μg
Expression SystemE. coli
Amino Acids1-166 aa
SequenceMGSSHHHHHHSSGLVPRGSHMASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEKLGGSAVISLEGKPL
Protein TagN-His
Predicted molecular weight20.7 kDa
SDS-PAGE21.2 kDa, reducing conditions; 21.2 kDa, non-reducing conditions

Images

Recombinant Human Cofilin Protein (rp169589) - SDS-PAGE
Recombinant Human Cofilin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 21.2 kDa.

Product Specifications

FormLyophilized
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C stable up to 1 year. Avoid freeze/thaw cycle.

Certificates

Certificate of Analysis(COA)

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4 results found

Lot NumberCertificate TypeDateItem
ZJ23F1201881Certificate of AnalysisDec 13, 2023 rp169589
ZJ23F1201880Certificate of AnalysisDec 13, 2023 rp169589
ZJ23F1201879Certificate of AnalysisDec 13, 2023 rp169589
ZJ23R1100167Certificate of AnalysisNov 24, 2023 rp169589

Related Documents

References

1. Nakashima S, Matsuda H, Kurume A, Oda Y, Nakamura S, Yamashita M, Yoshikawa M..  (2010)  Cucurbitacin E as a new inhibitor of cofilin phosphorylation in human leukemia U937 cells..  Bioorg Med Chem Lett,  20  (9): (2994-2997).  [PMID:20347305]
2. Ogawa, K K and 6 more authors..  (1990)  Coding sequence of human placenta cofilin cDNA..  Nucleic acids research,    (11):   [PMID:2263493]
3. van der Steege, G G and 9 more authors..  (1995)  A provisional transcript map of the spinal muscular atrophy (SMA) critical region..  European journal of human genetics : EJHG,      [PMID:7552146]
4. Davidson, M M MM and Haslam, R J RJ..  (1994)  Dephosphorylation of cofilin in stimulated platelets: roles for a GTP-binding protein and Ca2+..  The Biochemical journal,    (1):   [PMID:8037689]
5. Gillett, G T GT, Fox, M F MF, Rowe, P S PS, Casimir, C M CM and Povey, S S..  (1996)  Mapping of human non-muscle type cofilin (CFL1) to chromosome 11q13 and muscle-type cofilin (CFL2) to chromosome 14..  Annals of human genetics,      [PMID:8800436]
6. Yeoh, Sharon S, Pope, Brian B, Mannherz, Hans G HG and Weeds, Alan A..  (2002)  Determining the differences in actin binding by human ADF and cofilin..  Journal of molecular biology,    (25):   [PMID:11812157]
7. Gevaert, Kris K and 6 more authors..  (2003)  Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides..  Nature biotechnology,      [PMID:12665801]
8. Nakano, Kuniko K and 6 more authors..  (2003)  Cofilin phosphorylation and actin polymerization by NRK/NESK, a member of the germinal center kinase family..  Experimental cell research,    (15):   [PMID:12837278]
9. Pope, Brian J BJ, Zierler-Gould, Karen M KM, Kühne, Ronald R, Weeds, Alan G AG and Ball, Linda J LJ..  (2004)  Solution structure of human cofilin: actin binding, pH sensitivity, and relationship to actin-depolymerizing factor..  The Journal of biological chemistry,    (6):   [PMID:14627701]
10. Andersen, Jens S JS and 5 more authors..  (2003)  Proteomic characterization of the human centrosome by protein correlation profiling..  Nature,    (4):   [PMID:14654843]
11. Gohla, Antje A, Birkenfeld, Jörg J and Bokoch, Gary M GM..  (2005)  Chronophin, a novel HAD-type serine protein phosphatase, regulates cofilin-dependent actin dynamics..  Nature cell biology,      [PMID:15580268]
12. Leong, Wai Fook WF and Chow, Vincent T K VT..  (2006)  Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection..  Cellular microbiology,      [PMID:16548883]
13. Bai, Siau Wei SW and 9 more authors..  (2011)  Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration..  BMC biology,    (11):   [PMID:21834987]
14. Borroni, Elena M EM and 10 more authors..  (2013)  β-arrestin-dependent activation of the cofilin pathway is required for the scavenging activity of the atypical chemokine receptor D6..  Science signaling,    (30):   [PMID:23633677]
15. Kipper, Sarit S and 7 more authors..  (2015)  New host factors important for respiratory syncytial virus (RSV) replication revealed by a novel microfluidics screen for interactors of matrix (M) protein..  Molecular & cellular proteomics : MCP,      [PMID:25556234]
16. Prudent, Renaud R and 8 more authors..  (2018)  A quantitative proteomic analysis of cofilin phosphorylation in myeloid cells and its modulation using the LIM kinase inhibitor Pyr1..  PloS one,      [PMID:30550596]

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