Recombinant Human COX2 Protein, >90% (SDS-PAGE), high purity

Features and benefits
  • Expression System: E. coli
  • Protein Tag: N-His
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp176701
Grouped product items
SKUSizeAvailabilityPrice Qty
rp176701-10μg (Trial Size)
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10μg
In stock
$59.90
rp176701-100μg
100μg
In stock
$299.90
rp176701-1mg
1mg
In stock
$1,999.90

Carrier Free, >90% (SDS-PAGE), E.coli, His Tag, 90-227 aa

Basic Description

Product NameRecombinant Human COX2 Protein, >90% (SDS-PAGE), high purity
SynonymsCytochrome c oxidase polypeptide II | Cytochrome c oxidase subunit 2 | MT-CO2 | MT-CO2 | COII | COX2 | COXII | MTCO2
GradeAzide Free, Carrier Free
Product Description

Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description:
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Specifications & PurityCarrier Free, Azide Free, ≥90%(SDS-PAGE)
Purity>90% (SDS-PAGE)
Endotoxin Concentration<1.0 EU/μg
Expression SystemE. coli
Amino Acids90-227aa
SequenceMHHHHHHVNDPSLTIKSIGHQWYWTYEYTDYGGLIFNSYMLPPLFLEPGDLRLLDVDNRVVLPIEAPIRMMITSQDVLHSWAVPTLGLKTDAIPGRLNQTTFTATRPGVYYGQCSEICGANHSFMPIVLELIPLKIFEMGPVFTL
Protein TagN-His
Predicted molecular weight16.5 kDa
SDS-PAGE13.2 kDa, under reducing conditions

Images

Recombinant Human COX2 Protein (rp176701) - SDS-PAGE
3 μg/lane of Recombinant Human COX2 Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 13.2 kDa under reducing conditions.

Product Specifications

FormLyophilized
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C for 1 year. Avoid freeze/thaw cycle.

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0202703Certificate of AnalysisFeb 06, 2024 rp176701
ZJ24F0202704Certificate of AnalysisFeb 06, 2024 rp176701
ZJ24F0202705Certificate of AnalysisFeb 06, 2024 rp176701

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