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Recombinant Human CYR61/CCN1 Protein, ＞95%(SDS-PAGE, HPLC), high purity

≥95%(SDS-PAGE)

Features and benefits

Expression System: E.coli
Accession #: O00622
Protein Tag: No tag
Bioactivity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/3T3 cells is less than 3.0 μg/ml, corresponding to a specific activity of > 330 IU/mg.
Endotoxin Concentration: <1.0 EU/μg

Item Number

rp144926

Grouped product items
SKU	Size	Availability	Price
rp144926-10μg	10μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$69.90
rp144926-50μg	50μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$329.90
rp144926-100μg	100μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$599.90
rp144926-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$5,159.90
rp144926-250μg	250μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$1,699.90

Animal Free, >95%(SDS-PAGE, HPLC), Active, E.coli, No tag, 25-381 aa

View related series

Click to view protein information: CYR61

Accession#:O00622 CCN1 CYR61 Gene ID:3491

Basic Description

Product Name	Recombinant Human CYR61/CCN1 Protein, ＞95%(SDS-PAGE, HPLC), high purity
Synonyms	CCN family member 1 \| CCN1 \| CCN1IGF-binding protein 10 \| Cyr61 \| Cysteine-rich angiogenic inducer 61 \| cysteine-rich, angiogenic inducer, 61 \| GIG1 \| GIG1cysteine-rich heparin-binding protein 61 \| IBP-10 \| IGFBP-10 \| IGFBP10cysteine-rich, anigogenic indu
Grade	ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description	Purity > 95 % by SDS-PAGE and HPLC analyses. Function Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. Banckground: Cyr61, also known as CCN1, is a 40-45 kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration (1). Cyr61 consists of an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain (2). Mature human Cyr61 shares 93% amino acid sequence identity with mouse and rat Cyr61. It is widely expressed during development and in adult tissues (2, 3). Cyr61 associates with the extracellular matrix (ECM) and with many cell surface molecules including Integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1, Syndecan-4, and heparan sulfate proteoglycans (1, 3). Cyr61 mediates the adhesion and migration of multiple cell types and also promotes vascular endothelial cell tubule formation (4-6). Plasmin cleavage of ECM-bound Cyr61 releases a 28 kDa N-terminal fragment which retains the ability to promote endothelial cell migration (7). Cyr61 exhibits both tumorigenic and tumor suppressor properties. It is up-regulated and promotes tumorigenesis, angiogenesis, and metastasis in breast, renal, gastric, squamous cell, and colorectal carcinomas as well as in glioma (8-12). In contrast, when down-regulated, it suppresses tumor growth in endometrial, hepatic, and non-small cell lung cancers (8, 13, 14). Cyr61 is also up-regulated in injured skin and bone where it induces the expression of growth factors, cytokines, proteases, and integrins involved in wound repair (15, 16).
Specifications & Purity	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
Purity	＞95%(SDS-PAGE, HPLC)
Bioactivity	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/3T3 cells is less than 3.0 μg/ml, corresponding to a specific activity of > 330 IU/mg.
Endotoxin Concentration	<1.0 EU/μg
Expression System	E.coli
Species	Human
Amino Acids	25-381 aa
Protein Tag	No tag
Accession #	O00622
Predicted molecular weight	39.4 kDa
SDS-PAGE	39.4 kDa

Images

Recombinant Human CYR61/CCN1 Protein (rp144926)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/3T3 cells is less than 3.0 μg/mL, corresponding to a specific activity of > 330 IU/mg.

Recombinant Human CYR61/CCN1 Protein (rp144926)-SDS-PAGE
Recombinant Human CYR61/CCN1 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a single band at 39.4 kDa.

Product Specifications

Form	Lyophilized
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconst

Certificates

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4 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0600503	Certificate of Analysis	Mar 26, 2024	rp144926
ZJ23F0600504	Certificate of Analysis	Mar 26, 2024	rp144926
ZJ23F0600505	Certificate of Analysis	Mar 26, 2024	rp144926
ZJ23F0600506	Certificate of Analysis	Mar 26, 2024	rp144926

References

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334] [10.1021/op500134e]
2. Jay, P P and 5 more authors.. (1997) The human growth factor-inducible immediate early gene, CYR61, maps to chromosome 1p.. Oncogene, (10): [PMID:9135077]
3. Kireeva, M L ML, Lam, S C SC and Lau, L F LF.. (1998) Adhesion of human umbilical vein endothelial cells to the immediate-early gene product Cyr61 is mediated through integrin alphavbeta3.. The Journal of biological chemistry, (30): [PMID:9446626]
4. Martinerie, C C, Viegas-Pequignot, E E, Nguyen, V C VC and Perbal, B B.. (1997) Chromosomal mapping and expression of the human cyr61 gene in tumour cells from the nervous system.. Molecular pathology : MP, [PMID:9536281]
5. Jedsadayanmata, A A, Chen, C C CC, Kireeva, M L ML, Lau, L F LF and Lam, S C SC.. (1999) Activation-dependent adhesion of human platelets to Cyr61 and Fisp12/mouse connective tissue growth factor is mediated through integrin alpha(IIb)beta(3).. The Journal of biological chemistry, (20): [PMID:10446209]
6. Chen, N N, Chen, C C CC and Lau, L F LF.. (2000) Adhesion of human skin fibroblasts to Cyr61 is mediated through integrin alpha 6beta 1 and cell surface heparan sulfate proteoglycans.. The Journal of biological chemistry, (11): [PMID:10821835]
7. Albrecht, C C and 5 more authors.. (2000) Muscarinic acetylcholine receptors induce the expression of the immediate early growth regulatory gene CYR61.. The Journal of biological chemistry, (15): [PMID:10852911]
8. Grzeszkiewicz, T M TM, Kirschling, D J DJ, Chen, N N and Lau, L F LF.. (2001) CYR61 stimulates human skin fibroblast migration through Integrin alpha vbeta 5 and enhances mitogenesis through integrin alpha vbeta 3, independent of its carboxyl-terminal domain.. The Journal of biological chemistry, (15): [PMID:11287419]
9. Chen, C C CC, Mo, F E FE and Lau, L F LF.. (2001) The angiogenic factor Cyr61 activates a genetic program for wound healing in human skin fibroblasts.. The Journal of biological chemistry, (14): [PMID:11584015]
10. Leng, Esther E, Malcolm, Tom T, Tai, Georgia G, Estable, Mario M and Sadowski, Ivan I.. () Organization and expression of the Cyr61 gene in normal human fibroblasts.. Journal of biomedical science, [PMID:11810026]
11. Tagliabracci, Vincent S VS and 14 more authors.. (2015) A Single Kinase Generates the Majority of the Secreted Phosphoproteome.. Cell, (18): [PMID:26091039]

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