Recombinant Human Dkk-1 Protein

Features and benefits
  • Expression System: HEK293
  • Protein Tag: C-His
  • Bioactivity: Immobilized Recombinant Human Dkk-1 Protein (rp181236) at 1.0 μg/mL can bind Recombinant DKK1 Antibody (Ab099940) with the EC50 of 5.57 ng/mL.
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp181236
Grouped product items
SKUSizeAvailabilityPrice Qty
rp181236-10μg (Trial Size)
Apply for free trial size(?)
Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free!
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
rp181236-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$139.90
rp181236-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$219.90
rp181236-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

Animal Free, ≥95% (SDS-PAGE), Active, 293F cell, C-His tag, 2-266 aa

Basic Description

Product NameRecombinant Human Dkk-1 Protein
Synonymsdickkopf homolog 1 (Xenopus laevis) | dickkopf related protein-1 | Dickkopf-1 | dickkopf-related protein 1 | Dkk1 | Dkk-1 | hDkk-1 | SKdickkopf-1 like | dickkopf (Xenopus laevis) homolog 1
GradeActiBioPure™, Animal Free, Bioactive, Carrier Free
Product Description

Protein Function:

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues. Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 87%, 90%, and 91% aa sequence identity with mouse, rat, rabbit, and bovine Dkk-1, respectively. It also shares 42% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well-documented antagonists of the canonical Wnt signaling pathway. This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6. Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2. Dkk‑1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling. Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development. Other sites of expression include developing neurons, hair follicles, and the retina of the eye. The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis. Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively. In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, ≥95%(SDS-PAGE)
BioactivityImmobilized Recombinant Human Dkk-1 Protein (rp181236) at 1.0 μg/mL can bind Recombinant DKK1 Antibody (Ab099940) with the EC50 of 5.57 ng/mL.
Endotoxin Concentration<1.0 EU/μg
Expression SystemHEK293
Amino Acids2-266 aa
SequenceMALGAAGATRVFVAMVAAALGGHPLLGVSATLNSVLNSNAIKNLPPPLGGAAGHPGSAVSAAPGILYPGGNKYQTIDNYQPYPCAEDEECGTDEYCASPTRGGDAGVQICLACRKRRKRCMRHAMCCPGNYCKNGICVSSDQNHFRGEIEETITESFGNDHSTLDGYSRRTTLSSKMYHTKGQEGSVCLRSSDCASGLCCARHFWSKICKPVLKEGQVCTKHRRKGSHGLEIFQRCYCGEGLSCRIQKDHHQASN
Protein TagC-His
Predicted molecular weight26.6 kDa
SDS-PAGE37.3-47.6 kDa, under reducing conditions; 42.4-60.6 kDa, under non-reducing conditions

Images

Recombinant Human Dkk-1 Protein (rp181236) - ELISA
Immobilized Recombinant Human Dkk-1 Protein (rp181236) at 1.0 μg/mL can bind Recombinant DKK1 Antibody (Ab099940) with the EC₅₀ of 5.57 ng/mL.

Recombinant Human Dkk-1 Protein (rp181236) - SDS-PAGE
3 μg/lane of Recombinant Human Dkk-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 37.3-47.6 kDa under reducing conditions and 42.4-60.6 kDa under non-reducing conditions.

Product Specifications

ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C for 1 year. Avoid freeze / thaw cycle.

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateItem
ZJ24F0707695Certificate of AnalysisJul 05, 2024 rp181236
ZJ24F0707694Certificate of AnalysisJul 05, 2024 rp181236
ZJ24F0707693Certificate of AnalysisJul 05, 2024 rp181236
ZJ24R0600683Certificate of AnalysisJun 14, 2024 rp181236

Related Documents

Solution Calculators