Recombinant Human DNAM-1/CD226 Protein, ≥95% (SDS-PAGE), high purity

Features and benefits
  • Expression System: HEK293
  • Accession #: Q15762
  • Protein Tag: C-hFc & His
  • Bioactivity: Immobilized Recombinant Human CD226 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD155 with a linear range of 0.3-1.2 μg/mL.
  • Endotoxin Concentration: <0.1 EU/μg
Item Number
rp143887
Grouped product items
SKUSizeAvailabilityPrice Qty
rp143887-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$99.90
rp143887-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$419.90
rp143887-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$649.90
rp143887-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

Animal Free, ≥95% (SDS-PAGE), Active, 293F, C-hFc&His tag, 19-247 aa

Basic Description

Product NameRecombinant Human DNAM-1/CD226 Protein, ≥95% (SDS-PAGE), high purity
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description

Purity
≥95% (SDS-PAGE)
Endotoxin level
<0.1 EU/μg
Function
Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
Purity≥95% (SDS-PAGE)
BioactivityImmobilized Recombinant Human CD226 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD155 with a linear range of 0.3-1.2 μg/mL.
Endotoxin Concentration<0.1 EU/μg
Expression SystemHEK293
SpeciesHuman
Amino Acids19-247 aa
SequenceEEVLWHTSVPFAENMSLECVYPSMGILTQVEWFKIGTQQDSIAIFSPTHGMVIRKPYAERVYFLNSTMASNNMTLFFRNASEDDVGYYSCSLYTYPQGTWQKVIQVVQSDSFEAAVPSNSHIVSEPGKNVTLTCQPQMTWPVQAVRWEKIQPRQIDLLTYCNLVHGRNFTSKFPRQIVSNCSHGRWSVIVIPDVTVSDSGLYRCYLQASAGENETFVMRLTVAEGKTDNENLYFQGMDPKSCDKTHTCPPCPAPE
Protein TagC-hFc & His
Accession #Q15762
Predicted molecular weight54 kDa
SDS-PAGE80-90 kDa, under reducing conditions

Images

Recombinant Human DNAM-1/CD226 Protein (rp143887) - Protein Bioactivity
Immobilized Recombinant Human DNAM-1/CD226 Protein (rp143887) at 4.0 μg/mL can bind Biotinylated Human CD155 with a linear range of 0.02 - 0.625 μg/mL. The ED ₅₀ for this effect is 0.90 μg/mL.

Recombinant Human DNAM-1/CD226 Protein (rp143887) - SDS-PAGE
3 μg/lane of Recombinant Human DNAM-1/CD226 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 80.0 kDa under reducing conditions and 80.0 & 160.0 kDa under non-reducing conditions.

Product Specifications

FormLyophilized
ReconstitutionReconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0606788Certificate of AnalysisJun 14, 2024 rp143887
ZJ24F0606787Certificate of AnalysisJun 14, 2024 rp143887
ZJ24F0606786Certificate of AnalysisJun 14, 2024 rp143887

Related Documents

References

1. Zhu Y, Paniccia A, Schulick AC, Chen W, Koenig MR, Byers JT, Yao S, Bevers S, Edil BH.  (2016)  Identification of CD112R as a novel checkpoint for human T cells..  J Exp Med,  213  (2): (167-76).  [PMID:26755705]
2. Shibuya, A A and 10 more authors..  (1996)  DNAM-1, a novel adhesion molecule involved in the cytolytic function of T lymphocytes..  Immunity,      [PMID:8673704]
3. Tahara-Hanaoka, Satoko S and 8 more authors..  (2004)  Functional characterization of DNAM-1 (CD226) interaction with its ligands PVR (CD155) and nectin-2 (PRR-2/CD112)..  International immunology,      [PMID:15039383]
4. Pende, Daniela D and 13 more authors..  (2005)  PVR (CD155) and Nectin-2 (CD112) as ligands of the human DNAM-1 (CD226) activating receptor: involvement in tumor cell lysis..  Molecular immunology,      [PMID:15607800]
5. Nusbaum, Chad C and 54 more authors..  (2005)  DNA sequence and analysis of human chromosome 18..  Nature,    (22):   [PMID:16177791]
6. Zahedi, René P RP and 8 more authors..  (2008)  Phosphoproteome of resting human platelets..  Journal of proteome research,      [PMID:18088087]

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