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Recombinant Human EGF GMP Protein

Features and benefitsView More
  • Expression System: E. coli
  • Protein Tag: No tag/C-His tag
  • Bioactivity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 20‑100 pg/mL. The specific activity of Recombinant Human EGF is >8.0 x 10^5 IU/mg, which is calibrated against the human EGF WHO International Standard (NIBSC code: 91/530).
  • Endotoxin Concentration: <0.1 EU/μg
Item Number
rp168403-GMP
Grouped product items
SKUSizeAvailabilityPrice Qty
rp168403-GMP-200μg
200μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$629.90
rp168403-GMP-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,889.90

GMP, ≥97% (SDS-PAGE&SEC-HPLC), Active, E.coli, No tag, 971-1023 aa

Basic Description

Product NameRecombinant Human EGF GMP Protein
SynonymsEpidermal Growth Factor | beta-urogastrone | EGF | epidermal growth factor (beta-urogastrone) | hEGF | HOMG4 | pro-epidermal growth factor | URG | Urogastrone
GradeActiBioPure™, Animal Free, Bioactive, Carrier Free, GMP, High Performance
Specifications & PurityAnimal Free, Carrier Free, GMP, Bioactive, ActiBioPure™, High performance, ≥97%(SDS-PAGE&SEC-HPLC)
Biochemical and Physiological MechanismsEGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR
BioactivityMeasured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 20‑100 pg/mL. The specific activity of Recombinant Human EGF is >8.0 x 10^5 IU/mg, which is calibrated against the human EGF WHO International Standard (NIBSC code: 91/530).
Endotoxin Concentration<0.1 EU/μg
Expression SystemE. coli
Amino Acids971-1023 aa
SequenceMNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR
Protein TagNo tag/C-His tag
N-terminal SequenceMet-Asn971-Ser-Asp-Ser-Glu-(Cys)-Pro-Leu-Ser
Predicted molecular weight6 kDa
SDS-PAGE6 kDa under reducing conditions.

Product Specifications

ReconstitutionReconstitute at 200 μg/mL in PBS.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageA minimum of 12 months when stored at ≤ -20 ℃ as supplied. 1 month, 2 to 8 ℃ under sterile conditions after reconstitution. 3 months, ≤ -20 ℃ under sterile conditions after reconstitution. Upon delivery aliquot. Avoid freeze/thaw cycle.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

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Related Documents

Solution Calculators