Recombinant Human EGF Protein - Recombinant protein, CAS No.62253-63-8

Features and benefits
  • Expression System: Pichia pastoris
  • Accession #: P01133
  • Protein Tag: No tag
  • Bioactivity: Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.
  • Endotoxin Concentration: <0.01 EU/μg
Item Number
rp185510
Grouped product items
SKUSizeAvailabilityPrice Qty
rp185510-50μg (Trial Size)
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50μg
In stock
$119.90
rp185510-200μg
200μg
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$329.90
rp185510-1mg
1mg
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$999.90

GMP, ≥95% (SDS-PAGE), Active, Pichia pastoris, No tag, 971-1023 aa

Basic Description

Product NameRecombinant Human EGF Protein - Recombinant protein, CAS No.62253-63-8
SynonymsRecombinant Human Epidermal Growth Factor | rhEGF | beta-urogastrone | EGF | epidermal growth factor (beta-urogastrone | epidermal growth factor | hEGF | HOMG4 | pro-epidermal growth factor | URG | Urogastrone
GradeActiBioPure™, Azide Free, Bioactive, Carrier Free, GMP, High Performance
Product Description

Purity

≥95% SDS-PAGE

Additional sequence information

Mature chain

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

Post-translational

O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).

pH value: 6.5-7.5

Human epidermal cell growth factor, Mannitol, Sodium dihydrogen monohydrate phosphate, Disodium hydrogen dodecahydrate phosphate, Water 
Specifications & PurityActiBioPure™, Bioactive, GMP, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
BioactivityMeasured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.
Endotoxin Concentration<0.01 EU/μg
Expression SystemPichia pastoris
SpeciesHuman
Amino Acids971-1023 aa
Protein TagNo tag
Accession #P01133
Predicted molecular weight 6.5kD
SDS-PAGE7.1 kDa, under reducing conditions.

Product Specifications

FormLyophilized
ReconstitutionReconstitute in sterile water to a concentration of 100-200μg/ml, and further dilute it in other solvents. Upon reconstitution, the product could be stored at 4 ℃ for 2-7 days and used up as soon as possible. For future use, should be stored at -20 ℃.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C. Upon delivery aliquot. Avoid freeze/thaw cycle.
CAS62253-63-8

Certificates

Certificate of Analysis(COA)

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2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505243Certificate of AnalysisMay 09, 2024 rp185510
ZJ24F0505244Certificate of AnalysisMay 09, 2024 rp185510

Safety and Hazards(GHS)

Pictogram(s) GHS07
Signal Warning
Hazard Statements

H315:Causes skin irritation

H319:Causes serious eye irritation

H335:May cause respiratory irritation

Precautionary Statements

P261:Avoid breathing dust/fume/gas/mist/vapors/spray.

P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do - continue rinsing.

P280:Wear protective gloves/protective clothing/eye protection/face protection.

P302+P352:IF ON SKIN: wash with plenty of water.

P321:Specific treatment (see ... on this label).

P405:Store locked up.

P501:Dispose of contents/container to ...

P264:Wash hands [and …] thoroughly after handling.

P271:Use only outdoors or in a well-ventilated area.

P304+P340:IF INHALED: Remove person to fresh air and keep comfortable for breathing.

P403+P233:Store in a well-ventilated place. Keep container tightly closed.

P362+P364:Take off contaminated clothing and wash it before reuse.

P264+P265:Wash hands [and …] thoroughly after handling. Do not touch eyes.

P337+P317:If eye irritation persists: Get medical help.

P332+P317:If skin irritation occurs: Get medical help.

P319:Get medical help if you feel unwell.

WGK Germany 3

Related Documents

Citations of This Product

1. Cao Mingbo, Ren Yupeng, Li Yuxuan, Deng Junfeng, Su Xiaorui, Tang Yongchang, Yuan Feng, Deng Haixia, Yang Gaoyuan, He Zhiwei, Liu Bo, Yao Zhicheng, Deng Meihai.  (2023)  Lnc-ZEB2-19 Inhibits the Progression and Lenvatinib Resistance of Hepatocellular Carcinoma by Attenuating the NF-κB Signaling Pathway through the TRA2A/RSPH14 Axis.  International Journal of Biological Sciences,  19  (12): (3678-3693).  [PMID:37564197] [10.7150/ijbs.85270]
2. Maoze Guo, Yuqiu Wang, Bingbing Gao, Bingfang He.  (2021)  Shark Tooth-Inspired Microneedle Dressing for Intelligent Wound Management.  ACS Nano,  15  (9): (15316–15327).  [PMID:34533924] [10.1021/acsnano.1c06279]

References

1. Cao Mingbo, Ren Yupeng, Li Yuxuan, Deng Junfeng, Su Xiaorui, Tang Yongchang, Yuan Feng, Deng Haixia, Yang Gaoyuan, He Zhiwei, Liu Bo, Yao Zhicheng, Deng Meihai.  (2023)  Lnc-ZEB2-19 Inhibits the Progression and Lenvatinib Resistance of Hepatocellular Carcinoma by Attenuating the NF-κB Signaling Pathway through the TRA2A/RSPH14 Axis.  International Journal of Biological Sciences,  19  (12): (3678-3693).  [PMID:37564197] [10.7150/ijbs.85270]
2. Maoze Guo, Yuqiu Wang, Bingbing Gao, Bingfang He.  (2021)  Shark Tooth-Inspired Microneedle Dressing for Intelligent Wound Management.  ACS Nano,  15  (9): (15316–15327).  [PMID:34533924] [10.1021/acsnano.1c06279]

Solution Calculators