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50μg
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$119.90
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200μg
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$329.90
rp185510-1mg
1mg
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Recombinant Human EGF Protein - Recombinant protein, CAS No.62253-63-8
Grade
ActiBioPure™, Azide Free, Bioactive, Carrier Free, GMP, High Performance
Product Description
Purity
≥95% SDS-PAGE
Additional sequence information
Mature chain
Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
Post-translational
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
pH value: 6.5-7.5
Human epidermal cell growth factor, Mannitol, Sodium dihydrogen monohydrate phosphate, Disodium hydrogen dodecahydrate phosphate, Water
Specifications & Purity
ActiBioPure™, Bioactive, GMP, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.
Reconstitute in sterile water to a concentration of 100-200μg/ml, and further dilute it in other solvents. Upon reconstitution, the product could be stored at 4 ℃ for 2-7 days and used up as soon as possible. For future use, should be stored at -20 ℃.
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C. Upon delivery aliquot. Avoid freeze/thaw cycle.
CAS
62253-63-8
Certificates
Certificate of Analysis(COA)
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ZJ24F0505243