| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp145590-10μg | 10μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $69.90 | |
| rp145590-50μg | 50μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $309.90 | |
| rp145590-100μg | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $459.90 | |
| rp145590-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Animal Free , >95%(SDS-PAGE), Active, 293F, His tag, 24-265 aa
| Product Name | Recombinant Human EpCAM Protein, ＞95%(SDS-PAGE), high purity |
|---|---|
| Synonyms | 17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance |
| Product Description | Purity >95% SDS-PAGE Molecular weight informat 28-40kDa (SDS-PAGE under reducing conditions) Additional sequence information Extracellular domain. Function May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. Post-translational Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE) |
| Purity | ＞95%(SDS-PAGE) |
| Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to EpCAM-His coated plates (1.25μg/mL, 100μL/well), approximately >30% will adhere specifically after 30 minutes a |
| Endotoxin Concentration | ＜0.1 EU/μg |
| Expression System | HEK293 |
| Species | Human |
| Amino Acids | 24-265 aa |
| Sequence | QEECVCENYKLAVNCFVNNNRQCQCTSVGAQNTVICSKLAAKCLVMKAEMNGSKLGRRAKPEGALQNNDGLYDPDCDESGLFKAKQCNGTSMCWCVNTAGVRRTDKDTEITCSERVRTYWIIIELKHKAREKPYDSKSLRTALQKEITTRYQLDPKFITSILYENNVITIDLVQNSSQKTQNDVDIADVAYYFEKDVKGESLFHSKKMDLTVNGEQLDLDPGQTLIYYVDEKAPEFSMQGLKHHHHHH |
| Protein Tag | C-His |
| Accession # | P16422 |
| Predicted molecular weight | 26 kDa |
| SDS-PAGE | 31.5-44.0 kDa, under reducing condition. |
Recombinant Human EpCAM Protein (rp145590) - Protein Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of NIH/3T3 mouse embryonic fibroblast cells. When cells are added to EpCAM Protein coated plates (1.25μg/mL, 100μL/well), approximately >30% will adhere specifically after 30 minutes at 37℃.
Recombinant Human EpCAM Protein (rp145590) - SDS-PAGE
2.5 μg/lane of Recombinant Human EpCAM Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 31.5-44.0 kDa under reducing conditions.
| Form | Lyophilized |
|---|---|
| Reconstitution | Reconstitute in water to a concentration of 0.1-0.5 mg/ml. |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20~-80℃ for more than 1 year |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0700630 | Certificate of Analysis | Jul 21, 2023 | rp145590 |
| ZJ23F0700629 | Certificate of Analysis | Jul 20, 2023 | rp145590 |
| ZJ23F0700628 | Certificate of Analysis | Jul 20, 2023 | rp145590 |
| 1. Simon, B B and 5 more authors.. (1990) Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2108441] |
| 2. Szala, S S and 6 more authors.. (1990) Molecular cloning of cDNA for the carcinoma-associated antigen GA733-2.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2333300] |
| 3. Strnad, J J and 9 more authors.. (1989) Molecular cloning and characterization of a human adenocarcinoma/epithelial cell surface antigen complementary DNA.. Cancer research, (15): [PMID:2463074] |
| 4. Perez, M S MS and Walker, L E LE.. (1989) Isolation and characterization of a cDNA encoding the KS1/4 epithelial carcinoma marker.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:2469722] |
| 5. Linnenbach, A J AJ and 7 more authors.. (1989) Sequence investigation of the major gastrointestinal tumor-associated antigen gene family, GA733.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2911574] |
| 6. Björk, P P and 8 more authors.. (1993) Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody.. The Journal of biological chemistry, (15): [PMID:7693697] |
| 7. Linnenbach, A J AJ and 7 more authors.. (1993) Retroposition in a family of carcinoma-associated antigen genes.. Molecular and cellular biology, [PMID:8382772] |
| 8. Chong, J M JM and Speicher, D W DW.. (2001) Determination of disulfide bond assignments and N-glycosylation sites of the human gastrointestinal carcinoma antigen GA733-2 (CO17-1A, EGP, KS1-4, KSA, and Ep-CAM).. The Journal of biological chemistry, (23): [PMID:11080501] |
| 9. Münz, Markus M and 5 more authors.. (2004) The carcinoma-associated antigen EpCAM upregulates c-myc and induces cell proliferation.. Oncogene, (29): [PMID:15195135] |
| 10. Münz, Markus M, Zeidler, Reinhard R and Gires, Olivier O.. (2005) The tumour-associated antigen EpCAM upregulates the fatty acid binding protein E-FABP.. Cancer letters, (8): [PMID:15922867] |
| 11. Ladwein, Markus M and 8 more authors.. (2005) The cell-cell adhesion molecule EpCAM interacts directly with the tight junction protein claudin-7.. Experimental cell research, (1): [PMID:16054130] |
| 12. Munz, Markus M, Fellinger, Karin K, Hofmann, Tanja T, Schmitt, Barbel B and Gires, Olivier O.. (2008) Glycosylation is crucial for stability of tumour and cancer stem cell antigen EpCAM.. Frontiers in bioscience : a journal and virtual library, (1): [PMID:18508581] |
| 13. Sivagnanam, Mamata M and 15 more authors.. (2008) Identification of EpCAM as the gene for congenital tufting enteropathy.. Gastroenterology, [PMID:18572020] |
| 14. Ligtenberg, Marjolijn J L MJ and 17 more authors.. (2009) Heritable somatic methylation and inactivation of MSH2 in families with Lynch syndrome due to deletion of the 3' exons of TACSTD1.. Nature genetics, [PMID:19098912] |
| 15. Ng, Valerie Y VY, Ang, Sheu Ngo SN, Chan, Jia Xin JX and Choo, Andre B H AB.. (2010) Characterization of epithelial cell adhesion molecule as a surface marker on undifferentiated human embryonic stem cells.. Stem cells (Dayton, Ohio), [PMID:19785009] |
| 16. Lu, Tung-Ying TY and 6 more authors.. (2010) Epithelial cell adhesion molecule regulation is associated with the maintenance of the undifferentiated phenotype of human embryonic stem cells.. The Journal of biological chemistry, (19): [PMID:20064925] |
