| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp184031-10μg (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $139.90 | |
| rp184031-50μg | 50μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $399.90 | |
| rp184031-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $639.90 | |
| rp184031-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $3,199.90 |
Animal Free, ≥95% (SDS-PAGE), Active, 293F cell, C-His tag, 1-550 aa
| Product Name | Recombinant Human EphB3 Protein |
|---|---|
| Grade | ActiBioPure™, Animal Free, Bioactive, Carrier Free |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, ≥95%(SDS-PAGE) |
| Biochemical and Physiological Mechanisms | EphB3, also known as Cek10, Tyro6, Sek4, Hek2, and Mdk5, is a 130 kDa member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural o |
| Bioactivity | Immobilized Recombinant Human EphB3 Chimera Protein (rp184031) at 2.0 μg/mL can bind Recombinant Human Ephrin-B1 Protein (rp145638) with the EC50 of 139.2 ng/mL. |
| Endotoxin Concentration | <1 EU/μg |
| Expression System | HEK293 |
| Amino Acids | 1-550 aa |
| Sequence | MARARPPPPPSPPPGLLPLLPPLLLLPLLLLPAGCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDEAMNPIRTYQVCNVRESSQNNWLRTGFIWRRDVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPFWMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYKKCASTTAGFALFPETLTGAEPTSLVIAPGTCIPNAVEVSVPLKLY |
| Protein Tag | C-His |
| Predicted molecular weight | 56.8 kDa |
| SDS-PAGE | 59.8 kDa, under reducing conditions; 54.6 kDa, under non-reducing conditions |
Recombinant Human EphB3 Protein (rp184031) - Binding Activity
Immobilized Recombinant Human EphB3 Protein (rp184031) at 2.0 μg/mL can bind Recombinant Human Ephrin-B1 Protein (rp145638) with the EC50 of 139.2 ng/mL.
Recombinant Human EphB3 Protein (rp184031) - SDS-PAGE
3 μg/lane of Recombinant Human EphB3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 59.8 kDa under reducing conditions and 54.6 kDa under non-reducing conditions.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu |
|---|---|
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C for 1 year. Avoid freeze / thaw cycle. |
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